Limits...
In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.).

Sulusoglu M, Cavusoglu A - ScientificWorldJournal (2014)

Bottom Line: In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination.The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.).Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

View Article: PubMed Central - PubMed

Affiliation: Arslanbey Agricultural Vocational School, Kocaeli University, 41285 Kocaeli, Turkey ; Department of Horticulture, Graduate School of Natural and Applied Sciences, Kocaeli University, 41380 Kocaeli, Turkey.

ABSTRACT
Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

Show MeSH
Pollen tube growth on germinated pollen (a); ungerminated pollen without a pollen tube (b) (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4227381&req=5

fig3: Pollen tube growth on germinated pollen (a); ungerminated pollen without a pollen tube (b) (×400).

Mentions: Pollen grains did not develop a pollen tube in 0% and 5% sucrose including media, or there was only a very small tube and tube elongation increased with increasing amount of sucrose in the culture media (Figures 2(a), 2(b), 2(c), and 2(d)). Pollen tube formation was inhibited again in 20 g/L sucrose including media, and pollen tube length was decreased too (Figure 2(e)). In Figure 3, pollen tube formation of cherry laurel was given (Figure 3(a)) and ungerminated pollen without a pollen tube (Figure 3(b)) in the culture media was shown.


In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.).

Sulusoglu M, Cavusoglu A - ScientificWorldJournal (2014)

Pollen tube growth on germinated pollen (a); ungerminated pollen without a pollen tube (b) (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4227381&req=5

fig3: Pollen tube growth on germinated pollen (a); ungerminated pollen without a pollen tube (b) (×400).
Mentions: Pollen grains did not develop a pollen tube in 0% and 5% sucrose including media, or there was only a very small tube and tube elongation increased with increasing amount of sucrose in the culture media (Figures 2(a), 2(b), 2(c), and 2(d)). Pollen tube formation was inhibited again in 20 g/L sucrose including media, and pollen tube length was decreased too (Figure 2(e)). In Figure 3, pollen tube formation of cherry laurel was given (Figure 3(a)) and ungerminated pollen without a pollen tube (Figure 3(b)) in the culture media was shown.

Bottom Line: In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination.The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.).Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

View Article: PubMed Central - PubMed

Affiliation: Arslanbey Agricultural Vocational School, Kocaeli University, 41285 Kocaeli, Turkey ; Department of Horticulture, Graduate School of Natural and Applied Sciences, Kocaeli University, 41380 Kocaeli, Turkey.

ABSTRACT
Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

Show MeSH