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Novel arabinan and galactan oligosaccharides from dicotyledonous plants.

Wefers D, Tyl CE, Bunzel M - Front Chem (2014)

Bottom Line: To get a detailed insight into their fine structure, various oligosaccharides were isolated from quinoa, potato galactan, and sugar beet pulp after enzymatic treatment.Additionally, an oligosaccharide with two adjacent arabinofuranose units O2-substituted with two ferulic acid monomers was characterized.The isolated oligosaccharides gave further insight into the structures of pectic side chains and may have an impact on plant physiology and dietary fiber fermentation.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Chemistry and Phytochemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology Karlsruhe, Germany.

ABSTRACT
Arabinans and galactans are neutral pectic side chains and an important part of the cell walls of dicotyledonous plants. To get a detailed insight into their fine structure, various oligosaccharides were isolated from quinoa, potato galactan, and sugar beet pulp after enzymatic treatment. LC-MS(2) and one- and two-dimensional NMR spectroscopy were used for unambiguous structural characterization. It was demonstrated that arabinans contain β-(1→3)-linked arabinobiose as a side chain in quinoa seeds, while potato galactan was comprised of β-(1→4)-linked galactopyranoses which are interspersed with α-(1→4)-linked arabinopyranoses. Additionally, an oligosaccharide with two adjacent arabinofuranose units O2-substituted with two ferulic acid monomers was characterized. The isolated oligosaccharides gave further insight into the structures of pectic side chains and may have an impact on plant physiology and dietary fiber fermentation.

No MeSH data available.


HMBC spectrum and structure of compound 2. Diagnostic long range correlations are marked and explained in the text.
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Figure 4: HMBC spectrum and structure of compound 2. Diagnostic long range correlations are marked and explained in the text.

Mentions: The corresponding structures including the descriptors are shown in Figures 2, 4, 6. Chemical shifts are given in ppm, coupling constants in Hz. n.d., not determined.


Novel arabinan and galactan oligosaccharides from dicotyledonous plants.

Wefers D, Tyl CE, Bunzel M - Front Chem (2014)

HMBC spectrum and structure of compound 2. Diagnostic long range correlations are marked and explained in the text.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4226231&req=5

Figure 4: HMBC spectrum and structure of compound 2. Diagnostic long range correlations are marked and explained in the text.
Mentions: The corresponding structures including the descriptors are shown in Figures 2, 4, 6. Chemical shifts are given in ppm, coupling constants in Hz. n.d., not determined.

Bottom Line: To get a detailed insight into their fine structure, various oligosaccharides were isolated from quinoa, potato galactan, and sugar beet pulp after enzymatic treatment.Additionally, an oligosaccharide with two adjacent arabinofuranose units O2-substituted with two ferulic acid monomers was characterized.The isolated oligosaccharides gave further insight into the structures of pectic side chains and may have an impact on plant physiology and dietary fiber fermentation.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Chemistry and Phytochemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology Karlsruhe, Germany.

ABSTRACT
Arabinans and galactans are neutral pectic side chains and an important part of the cell walls of dicotyledonous plants. To get a detailed insight into their fine structure, various oligosaccharides were isolated from quinoa, potato galactan, and sugar beet pulp after enzymatic treatment. LC-MS(2) and one- and two-dimensional NMR spectroscopy were used for unambiguous structural characterization. It was demonstrated that arabinans contain β-(1→3)-linked arabinobiose as a side chain in quinoa seeds, while potato galactan was comprised of β-(1→4)-linked galactopyranoses which are interspersed with α-(1→4)-linked arabinopyranoses. Additionally, an oligosaccharide with two adjacent arabinofuranose units O2-substituted with two ferulic acid monomers was characterized. The isolated oligosaccharides gave further insight into the structures of pectic side chains and may have an impact on plant physiology and dietary fiber fermentation.

No MeSH data available.