Limits...
Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH

Related in: MedlinePlus

Effect of different surfactants on amylase production and stability. The control flask does not contain any surfactant. Test flasks contained different surfactant in the medium at a level of 1.0% (w/v). The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed and for stability enzyme was preincubated with different surfactants (1.0%) at 55°C for 1 h and assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4226188&req=5

fig12: Effect of different surfactants on amylase production and stability. The control flask does not contain any surfactant. Test flasks contained different surfactant in the medium at a level of 1.0% (w/v). The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed and for stability enzyme was preincubated with different surfactants (1.0%) at 55°C for 1 h and assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.

Mentions: None of the surfactants tested had a pronounced inhibitory effect on enzyme activities. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity of Bacillus tequilensis RG-01 (Figure 12). On the other hand, the addition of SDS, Tween-40, Tween-60, and Tween-80 (1%) was found to stimulate activity by 20, 10, 15, and 9%, respectively. The stimulatory effect of surfactants on amylase activity is controversial. Oberoi et al. [41] also reported that the activity of amylase of Bacillus sp. was stimulated in the presence of Tween-80 (1%). Arnesen et al. [42] reported that amylase activity of Thermomyces lanuginosus was slightly inhibited by Triton X-100. A stimulatory effect of SDS (6%) on amylase activity and stability in Bacillus sp. strain TS-23 was documented by Lo et al. [43]. From the previous reports and the present study, it was evident that the same surfactant could have different effects on the activity of the same enzyme in the same microorganism or on the activity of different enzymes in the same microorganism.


Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Effect of different surfactants on amylase production and stability. The control flask does not contain any surfactant. Test flasks contained different surfactant in the medium at a level of 1.0% (w/v). The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed and for stability enzyme was preincubated with different surfactants (1.0%) at 55°C for 1 h and assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226188&req=5

fig12: Effect of different surfactants on amylase production and stability. The control flask does not contain any surfactant. Test flasks contained different surfactant in the medium at a level of 1.0% (w/v). The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed and for stability enzyme was preincubated with different surfactants (1.0%) at 55°C for 1 h and assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
Mentions: None of the surfactants tested had a pronounced inhibitory effect on enzyme activities. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity of Bacillus tequilensis RG-01 (Figure 12). On the other hand, the addition of SDS, Tween-40, Tween-60, and Tween-80 (1%) was found to stimulate activity by 20, 10, 15, and 9%, respectively. The stimulatory effect of surfactants on amylase activity is controversial. Oberoi et al. [41] also reported that the activity of amylase of Bacillus sp. was stimulated in the presence of Tween-80 (1%). Arnesen et al. [42] reported that amylase activity of Thermomyces lanuginosus was slightly inhibited by Triton X-100. A stimulatory effect of SDS (6%) on amylase activity and stability in Bacillus sp. strain TS-23 was documented by Lo et al. [43]. From the previous reports and the present study, it was evident that the same surfactant could have different effects on the activity of the same enzyme in the same microorganism or on the activity of different enzymes in the same microorganism.

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH
Related in: MedlinePlus