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Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

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Effect of different concentration of metal ions (NaCl, CaCl2, and MgSO4) on amylase production. Test flasks contained different concentrations (5 mM to 45 mM) of metal ions (NaCl, CaCl2, and MgSO4) in the medium. The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
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fig11: Effect of different concentration of metal ions (NaCl, CaCl2, and MgSO4) on amylase production. Test flasks contained different concentrations (5 mM to 45 mM) of metal ions (NaCl, CaCl2, and MgSO4) in the medium. The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.

Mentions: Different concentrations of NaCl, CaCl2, and MgSO4 (5, 10, 15, 25, 35, and 45 mM) in the medium were also tested for amylase production at the same growth condition at which metal ion sources were evaluated. Bacteria showed same pattern of amylase production with NaCl, CaCl2, and MgSO4 at 25 Mm concentration but maximum amylase production was reported from the CaCl2. By further increase and decrease in the concentration of all three metal ions, enzyme production was reduced (Figure 11).


Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Effect of different concentration of metal ions (NaCl, CaCl2, and MgSO4) on amylase production. Test flasks contained different concentrations (5 mM to 45 mM) of metal ions (NaCl, CaCl2, and MgSO4) in the medium. The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226188&req=5

fig11: Effect of different concentration of metal ions (NaCl, CaCl2, and MgSO4) on amylase production. Test flasks contained different concentrations (5 mM to 45 mM) of metal ions (NaCl, CaCl2, and MgSO4) in the medium. The flasks were inoculated with culture and were incubated at initial pH 7.0, 55°C for 24 h. For enzyme activity, the reaction was assayed by standard assay method. Error bars presented mean values of ±standard deviation of triplicates of three independent experiments.
Mentions: Different concentrations of NaCl, CaCl2, and MgSO4 (5, 10, 15, 25, 35, and 45 mM) in the medium were also tested for amylase production at the same growth condition at which metal ion sources were evaluated. Bacteria showed same pattern of amylase production with NaCl, CaCl2, and MgSO4 at 25 Mm concentration but maximum amylase production was reported from the CaCl2. By further increase and decrease in the concentration of all three metal ions, enzyme production was reduced (Figure 11).

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH
Related in: MedlinePlus