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Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

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Related in: MedlinePlus

Effect of different nitrogen sources on amylase production. The control flask does not contain any nitrogen sources. Test flasks contained different nitrogen sources in the medium at a level of 0.5% (w/v). The flasks were inoculated with culture and incubated at 55°C for 24 h at pH 7.0 with 4.0% starch. Error bars presented are mean values of ±standard deviation of triplicates of three independent experiments.
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fig8: Effect of different nitrogen sources on amylase production. The control flask does not contain any nitrogen sources. Test flasks contained different nitrogen sources in the medium at a level of 0.5% (w/v). The flasks were inoculated with culture and incubated at 55°C for 24 h at pH 7.0 with 4.0% starch. Error bars presented are mean values of ±standard deviation of triplicates of three independent experiments.

Mentions: Inorganic and organic nitrogen, viz, peptone, beef extract, yeast extract, urea, ammonium sulphate, ammonium chloride, and ammonium hydrogen phosphate, at the rate of 0.5% (w/v) were used in the basal medium for amylase production by Bacillus tequilensis RG-01 (Figure 8). The enzyme production by the isolate was almost similar in beef extract and peptone amended medium (6000 U/mL) followed by yeast extract and ammonium sulphate. Similarly, Thippeswamy et al. [32] reported that the addition of peptone increases amylase production by Bacillus sp. Bacillus tequilensis RG-01 also showed better amylase production in the presence of ammonium chloride and ammonium hydrogen phosphate. Narang and Satyanarayana [28] also reported that ammonium chloride and ammonium hydrogen phosphate favoured growth and enzyme secretion by bacterial strains. Other nitrogen source, like urea, showed inhibitory effect on amylase production of Bacillus tequilensis RG-01. Ramachandran et al. [26] have already reported that supplementation of urea at 1% concentration resulted in a decrease in amylase production.


Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Effect of different nitrogen sources on amylase production. The control flask does not contain any nitrogen sources. Test flasks contained different nitrogen sources in the medium at a level of 0.5% (w/v). The flasks were inoculated with culture and incubated at 55°C for 24 h at pH 7.0 with 4.0% starch. Error bars presented are mean values of ±standard deviation of triplicates of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226188&req=5

fig8: Effect of different nitrogen sources on amylase production. The control flask does not contain any nitrogen sources. Test flasks contained different nitrogen sources in the medium at a level of 0.5% (w/v). The flasks were inoculated with culture and incubated at 55°C for 24 h at pH 7.0 with 4.0% starch. Error bars presented are mean values of ±standard deviation of triplicates of three independent experiments.
Mentions: Inorganic and organic nitrogen, viz, peptone, beef extract, yeast extract, urea, ammonium sulphate, ammonium chloride, and ammonium hydrogen phosphate, at the rate of 0.5% (w/v) were used in the basal medium for amylase production by Bacillus tequilensis RG-01 (Figure 8). The enzyme production by the isolate was almost similar in beef extract and peptone amended medium (6000 U/mL) followed by yeast extract and ammonium sulphate. Similarly, Thippeswamy et al. [32] reported that the addition of peptone increases amylase production by Bacillus sp. Bacillus tequilensis RG-01 also showed better amylase production in the presence of ammonium chloride and ammonium hydrogen phosphate. Narang and Satyanarayana [28] also reported that ammonium chloride and ammonium hydrogen phosphate favoured growth and enzyme secretion by bacterial strains. Other nitrogen source, like urea, showed inhibitory effect on amylase production of Bacillus tequilensis RG-01. Ramachandran et al. [26] have already reported that supplementation of urea at 1% concentration resulted in a decrease in amylase production.

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH
Related in: MedlinePlus