Limits...
Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH

Related in: MedlinePlus

Growth behavior of Bacillus tequilensis RG-01 and production of extracellular amylase, respectively, in starch nutrient media and modified starch nutrient media at initial pH 7.0 and 55°C and 120 rpm during 48 h growth.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4226188&req=5

fig2: Growth behavior of Bacillus tequilensis RG-01 and production of extracellular amylase, respectively, in starch nutrient media and modified starch nutrient media at initial pH 7.0 and 55°C and 120 rpm during 48 h growth.

Mentions: The RG-01 strain exhibited typical sigmoidal growth behavior in both culture media. In nutrient starch broth, stationary phase commenced at 24 h, while, in modified nutrient starch broth, the onset of stationary phase was at 20 h onward after a steep log phase. In both media, the stationary phase of bacterial growth witnessed maximum amylase production. However, the amylase production was the maximum (3500 U/mL) in modified nutrient starch medium at 20 h, while it was 2700 U/mL at 24 h in nutrient starch medium (Figure 2). Hence, modified nutrient starch medium was selected for further studies on amylase production.


Enhanced production and characterization of a solvent stable amylase from solvent tolerant Bacillus tequilensis RG-01: thermostable and surfactant resistant.

Tiwari S, Shukla N, Mishra P, Gaur R - ScientificWorldJournal (2014)

Growth behavior of Bacillus tequilensis RG-01 and production of extracellular amylase, respectively, in starch nutrient media and modified starch nutrient media at initial pH 7.0 and 55°C and 120 rpm during 48 h growth.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226188&req=5

fig2: Growth behavior of Bacillus tequilensis RG-01 and production of extracellular amylase, respectively, in starch nutrient media and modified starch nutrient media at initial pH 7.0 and 55°C and 120 rpm during 48 h growth.
Mentions: The RG-01 strain exhibited typical sigmoidal growth behavior in both culture media. In nutrient starch broth, stationary phase commenced at 24 h, while, in modified nutrient starch broth, the onset of stationary phase was at 20 h onward after a steep log phase. In both media, the stationary phase of bacterial growth witnessed maximum amylase production. However, the amylase production was the maximum (3500 U/mL) in modified nutrient starch medium at 20 h, while it was 2700 U/mL at 24 h in nutrient starch medium (Figure 2). Hence, modified nutrient starch medium was selected for further studies on amylase production.

Bottom Line: The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively.The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively.Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Centre of Excellence, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh 224001, India.

ABSTRACT
Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified as Bacillus tequilensis from MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca(2+) ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensis RG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

Show MeSH
Related in: MedlinePlus