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Biobleaching of industrial important dyes with peroxidase partially purified from garlic.

Osuji AC, Eze SO, Osayi EE, Chilaka FC - ScientificWorldJournal (2014)

Bottom Line: The Km and V max for H2O2 and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively.Peroxidase from garlic was effective in decolourizing Vat Yellow 2, Vat Orange 11, and Vat Black 27 better than Vat Green 9 dye.For all the parameters monitored, the decolourization was more effective at a pH range, temperature, H2O2 concentration, and enzyme concentration of 4.5-5.0, 50°C, 0.6 mM, and 0.20 U/mL, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Nigeria, Nsukka, Nigeria.

ABSTRACT
An acidic peroxidase was extracted from garlic (Allium sativum) and was partially purified threefold by ammonium sulphate precipitation, dialysis, and gel filtration chromatography using sephadex G-200. The specific activity of the enzyme increased from 4.89 U/mg after ammonium sulphate precipitation to 25.26 U/mg after gel filtration chromatography. The optimum temperature and pH of the enzyme were 50°C and 5.0, respectively. The Km and V max for H2O2 and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively. Peroxidase from garlic was effective in decolourizing Vat Yellow 2, Vat Orange 11, and Vat Black 27 better than Vat Green 9 dye. For all the parameters monitored, the decolourization was more effective at a pH range, temperature, H2O2 concentration, and enzyme concentration of 4.5-5.0, 50°C, 0.6 mM, and 0.20 U/mL, respectively. The observed properties of the enzyme together with its low cost of extraction (from local sources) show the potential of this enzyme for practical application in industrial wastewater treatment especially with hydrogen peroxide. These Vat dyes also exhibited potentials of acting as peroxidase inhibitors at alkaline pH range.

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Percentage dye decolourization after 4 h of incubation.
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fig8: Percentage dye decolourization after 4 h of incubation.

Mentions: The decolourization potential of garlic peroxidase was tested using four Vat dyes—Vat Yellow 2, Vat Orange 11, Vat Green 9, and Vat Black 27. After 4 h of incubation in an MRC stainless steel water bath model WBO-200 at 50°C (Figure 8), the percentage decolourization for each dye was found to be 83.5, 85.6, 48, and 81% for Vat Yellow 2, Vat Orange 11, Vat Green 9, and Vat Black 27, respectively. Subramaniam et al. [27] reported 23% decolourization of tannery effluent dyes using Momordica charantia peroxidase, after 4 h of incubation at 50°C. Onder et al. [23] recorded 90% decolourization of naphthol blue after 5 min of contact time at the temperature of 40°C using horseradish peroxidase. Husain et al. [28] recorded 85% decolourization of textile effluent dyes after 5 h incubation at 40°C using fenugreek peroxidase. The differences in time course of removal of these dyes as reported by various scholars might be due to the structural barrier and electron localization among the dyes. Also, the level of purification of the peroxidase used for biobleaching is an important factor to be considered.


Biobleaching of industrial important dyes with peroxidase partially purified from garlic.

Osuji AC, Eze SO, Osayi EE, Chilaka FC - ScientificWorldJournal (2014)

Percentage dye decolourization after 4 h of incubation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226186&req=5

fig8: Percentage dye decolourization after 4 h of incubation.
Mentions: The decolourization potential of garlic peroxidase was tested using four Vat dyes—Vat Yellow 2, Vat Orange 11, Vat Green 9, and Vat Black 27. After 4 h of incubation in an MRC stainless steel water bath model WBO-200 at 50°C (Figure 8), the percentage decolourization for each dye was found to be 83.5, 85.6, 48, and 81% for Vat Yellow 2, Vat Orange 11, Vat Green 9, and Vat Black 27, respectively. Subramaniam et al. [27] reported 23% decolourization of tannery effluent dyes using Momordica charantia peroxidase, after 4 h of incubation at 50°C. Onder et al. [23] recorded 90% decolourization of naphthol blue after 5 min of contact time at the temperature of 40°C using horseradish peroxidase. Husain et al. [28] recorded 85% decolourization of textile effluent dyes after 5 h incubation at 40°C using fenugreek peroxidase. The differences in time course of removal of these dyes as reported by various scholars might be due to the structural barrier and electron localization among the dyes. Also, the level of purification of the peroxidase used for biobleaching is an important factor to be considered.

Bottom Line: The Km and V max for H2O2 and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively.Peroxidase from garlic was effective in decolourizing Vat Yellow 2, Vat Orange 11, and Vat Black 27 better than Vat Green 9 dye.For all the parameters monitored, the decolourization was more effective at a pH range, temperature, H2O2 concentration, and enzyme concentration of 4.5-5.0, 50°C, 0.6 mM, and 0.20 U/mL, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Nigeria, Nsukka, Nigeria.

ABSTRACT
An acidic peroxidase was extracted from garlic (Allium sativum) and was partially purified threefold by ammonium sulphate precipitation, dialysis, and gel filtration chromatography using sephadex G-200. The specific activity of the enzyme increased from 4.89 U/mg after ammonium sulphate precipitation to 25.26 U/mg after gel filtration chromatography. The optimum temperature and pH of the enzyme were 50°C and 5.0, respectively. The Km and V max for H2O2 and o-dianisidine were 0.026 mM and 0.8 U/min, and 25 mM and 0.75 U/min, respectively. Peroxidase from garlic was effective in decolourizing Vat Yellow 2, Vat Orange 11, and Vat Black 27 better than Vat Green 9 dye. For all the parameters monitored, the decolourization was more effective at a pH range, temperature, H2O2 concentration, and enzyme concentration of 4.5-5.0, 50°C, 0.6 mM, and 0.20 U/mL, respectively. The observed properties of the enzyme together with its low cost of extraction (from local sources) show the potential of this enzyme for practical application in industrial wastewater treatment especially with hydrogen peroxide. These Vat dyes also exhibited potentials of acting as peroxidase inhibitors at alkaline pH range.

Show MeSH