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Identification of chemokines and growth factors in proliferative diabetic retinopathy vitreous.

Dai Y, Wu Z, Wang F, Zhang Z, Yu M - Biomed Res Int (2014)

Bottom Line: The significantly higher levels of CCL4 and CCL11 in PDR contradict the results of previous reports.Based on Spearman's nonparametric test, moderate-to-strong correlations were found between VEGF and other mediators.Our results indicate that these chemokines and growth factors could be candidates for research into targeted therapies applied either singly or in combination with anti-VEGF drugs for the treatment of PDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Wuxi No. 2 Hospital, Nanjing Medical University, Zhongshan 68, Wuxi 214002, China.

ABSTRACT
Associations were investigated between levels of chemokines and growth factors in the vitreous and proliferative diabetic retinopathy (PDR). Enrolled were 58 patients (58 eyes) requiring pars plana vitrectomy (PPV), with PDR (n=32, none with traction retinal detachment) or not (non-PDR). In the latter, 16 had macular hole (MH) and 10 had epiretinal membrane (ERM). With a multiplex bead immunoassay, levels of 11 chemokines and growth factors were measured from the undiluted vitreous sample from each patient. In the non-PDR eyes, the levels of the 11 chemokines and growth factors tested were similar between patients with MH and those with ERM. However, the levels of all 11 were significantly higher in the PDR eyes relative to the non-PDR; CCL17, CCL19, and TGFβ3 were markedly upregulated and have not been investigated in PDR previously. The significantly higher levels of CCL4 and CCL11 in PDR contradict the results of previous reports. Based on Spearman's nonparametric test, moderate-to-strong correlations were found between VEGF and other mediators. Our results indicate that these chemokines and growth factors could be candidates for research into targeted therapies applied either singly or in combination with anti-VEGF drugs for the treatment of PDR.

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Related in: MedlinePlus

Scatter plots of chemokines and growth factors levels in PDR vitreous. By multiplex beads immunoassay, significant elevation of all 11 mediators was found in PDR vitreous as compared with controls. Sixteen MH and 10 ERM patients constituted the non-PDR group. Among 11 mediators, CCL17, CCL19, and TGFβ3 were identified to be associated with PDR firstly. The vertical bars represent median with range.
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fig1: Scatter plots of chemokines and growth factors levels in PDR vitreous. By multiplex beads immunoassay, significant elevation of all 11 mediators was found in PDR vitreous as compared with controls. Sixteen MH and 10 ERM patients constituted the non-PDR group. Among 11 mediators, CCL17, CCL19, and TGFβ3 were identified to be associated with PDR firstly. The vertical bars represent median with range.

Mentions: Expect for CCL17, all the mediators tested could be detected in the vitreous of MH, ERM, and PDR patients. In the PDR patients, the CCL2 levels exceeded the maximum detection limit in approximately 31% (10/32) of samples. According to the results from the nonparametric Mann-Whitney test, all of the chemokines tested were found at significantly higher levels in the PDR group than in the non-PDR. CCL17 and CCL19 have never been investigated in PDR vitreous before, and we found that while CCL17 was present at low levels in PDR patients; this chemokine was undetectable in the majority of the non-PDR patients. Regarding CCL19, an obvious difference was found in the vitreous with median levels of 219.67 pg/mL in PDR patients but 41 pg/mL in controls. Associations between CCL4 and PDR and CCL11 and PDR have previously been equivocal [2, 15, 17]. In the present study, the median levels of CCL4 and CCL11 in the PDR group were approximately 1.3-fold and 1.2-fold, respectively, that of the controls (Figure 1).


Identification of chemokines and growth factors in proliferative diabetic retinopathy vitreous.

Dai Y, Wu Z, Wang F, Zhang Z, Yu M - Biomed Res Int (2014)

Scatter plots of chemokines and growth factors levels in PDR vitreous. By multiplex beads immunoassay, significant elevation of all 11 mediators was found in PDR vitreous as compared with controls. Sixteen MH and 10 ERM patients constituted the non-PDR group. Among 11 mediators, CCL17, CCL19, and TGFβ3 were identified to be associated with PDR firstly. The vertical bars represent median with range.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4226181&req=5

fig1: Scatter plots of chemokines and growth factors levels in PDR vitreous. By multiplex beads immunoassay, significant elevation of all 11 mediators was found in PDR vitreous as compared with controls. Sixteen MH and 10 ERM patients constituted the non-PDR group. Among 11 mediators, CCL17, CCL19, and TGFβ3 were identified to be associated with PDR firstly. The vertical bars represent median with range.
Mentions: Expect for CCL17, all the mediators tested could be detected in the vitreous of MH, ERM, and PDR patients. In the PDR patients, the CCL2 levels exceeded the maximum detection limit in approximately 31% (10/32) of samples. According to the results from the nonparametric Mann-Whitney test, all of the chemokines tested were found at significantly higher levels in the PDR group than in the non-PDR. CCL17 and CCL19 have never been investigated in PDR vitreous before, and we found that while CCL17 was present at low levels in PDR patients; this chemokine was undetectable in the majority of the non-PDR patients. Regarding CCL19, an obvious difference was found in the vitreous with median levels of 219.67 pg/mL in PDR patients but 41 pg/mL in controls. Associations between CCL4 and PDR and CCL11 and PDR have previously been equivocal [2, 15, 17]. In the present study, the median levels of CCL4 and CCL11 in the PDR group were approximately 1.3-fold and 1.2-fold, respectively, that of the controls (Figure 1).

Bottom Line: The significantly higher levels of CCL4 and CCL11 in PDR contradict the results of previous reports.Based on Spearman's nonparametric test, moderate-to-strong correlations were found between VEGF and other mediators.Our results indicate that these chemokines and growth factors could be candidates for research into targeted therapies applied either singly or in combination with anti-VEGF drugs for the treatment of PDR.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Wuxi No. 2 Hospital, Nanjing Medical University, Zhongshan 68, Wuxi 214002, China.

ABSTRACT
Associations were investigated between levels of chemokines and growth factors in the vitreous and proliferative diabetic retinopathy (PDR). Enrolled were 58 patients (58 eyes) requiring pars plana vitrectomy (PPV), with PDR (n=32, none with traction retinal detachment) or not (non-PDR). In the latter, 16 had macular hole (MH) and 10 had epiretinal membrane (ERM). With a multiplex bead immunoassay, levels of 11 chemokines and growth factors were measured from the undiluted vitreous sample from each patient. In the non-PDR eyes, the levels of the 11 chemokines and growth factors tested were similar between patients with MH and those with ERM. However, the levels of all 11 were significantly higher in the PDR eyes relative to the non-PDR; CCL17, CCL19, and TGFβ3 were markedly upregulated and have not been investigated in PDR previously. The significantly higher levels of CCL4 and CCL11 in PDR contradict the results of previous reports. Based on Spearman's nonparametric test, moderate-to-strong correlations were found between VEGF and other mediators. Our results indicate that these chemokines and growth factors could be candidates for research into targeted therapies applied either singly or in combination with anti-VEGF drugs for the treatment of PDR.

Show MeSH
Related in: MedlinePlus