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Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation.

Alcaraz M, Quesada S, Armero D, Martin-Gíl R, Olivares A, Achel GD - Colomb. Med. (2014)

Bottom Line: Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001).However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

View Article: PubMed Central - PubMed

Affiliation: Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.

ABSTRACT

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001). However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

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Related in: MedlinePlus

Genotoxic effect (Frequency of MN/500CB) of different volumes of sevofluorane and radiation: C control, RO Rosmarinic acid, DMSO Dimethyl sulfoxide and S5, S20, S40 different volumes of sevofluorane (µL) administered alone or before X-irradiation ((1) p <0.001 versus non-irradiated control, (2) p <0.001 versus irradiated control, (3) p< 0.01 versus irradiated control, (4) p <0.001 versus S5 non-irradiated.
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f02: Genotoxic effect (Frequency of MN/500CB) of different volumes of sevofluorane and radiation: C control, RO Rosmarinic acid, DMSO Dimethyl sulfoxide and S5, S20, S40 different volumes of sevofluorane (µL) administered alone or before X-irradiation ((1) p <0.001 versus non-irradiated control, (2) p <0.001 versus irradiated control, (3) p< 0.01 versus irradiated control, (4) p <0.001 versus S5 non-irradiated.

Mentions: In the genotoxic study, the basal frequency of the MN/500 CB was 10±2 MN/500 CB for the non-irradiated control of the human lymphocytes used in the cytome assay. Irradiation with 2 Gy of X-rays produced a significant increase in the appearance of MN, which reached 28±4 MN/500 CB (p <0.001), expressing a genotoxic damage induced by the X-rays (Fig. 2). The administration of RO and DMSO used as positive control of a radioprotective agent, led to a significant drop in the frequency of MN when administered before irradiation (p <0.001 and p <0.01, respectively). This expresses the genoprotective capacity of these substances against X-ray induced chromosome damage (Fig. 2), and demonstrate protection factors of 53.6% and 18.0% respectively.


Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation.

Alcaraz M, Quesada S, Armero D, Martin-Gíl R, Olivares A, Achel GD - Colomb. Med. (2014)

Genotoxic effect (Frequency of MN/500CB) of different volumes of sevofluorane and radiation: C control, RO Rosmarinic acid, DMSO Dimethyl sulfoxide and S5, S20, S40 different volumes of sevofluorane (µL) administered alone or before X-irradiation ((1) p <0.001 versus non-irradiated control, (2) p <0.001 versus irradiated control, (3) p< 0.01 versus irradiated control, (4) p <0.001 versus S5 non-irradiated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225786&req=5

f02: Genotoxic effect (Frequency of MN/500CB) of different volumes of sevofluorane and radiation: C control, RO Rosmarinic acid, DMSO Dimethyl sulfoxide and S5, S20, S40 different volumes of sevofluorane (µL) administered alone or before X-irradiation ((1) p <0.001 versus non-irradiated control, (2) p <0.001 versus irradiated control, (3) p< 0.01 versus irradiated control, (4) p <0.001 versus S5 non-irradiated.
Mentions: In the genotoxic study, the basal frequency of the MN/500 CB was 10±2 MN/500 CB for the non-irradiated control of the human lymphocytes used in the cytome assay. Irradiation with 2 Gy of X-rays produced a significant increase in the appearance of MN, which reached 28±4 MN/500 CB (p <0.001), expressing a genotoxic damage induced by the X-rays (Fig. 2). The administration of RO and DMSO used as positive control of a radioprotective agent, led to a significant drop in the frequency of MN when administered before irradiation (p <0.001 and p <0.01, respectively). This expresses the genoprotective capacity of these substances against X-ray induced chromosome damage (Fig. 2), and demonstrate protection factors of 53.6% and 18.0% respectively.

Bottom Line: Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001).However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

View Article: PubMed Central - PubMed

Affiliation: Radiology and Physical Medicine Department. Faculty of Medicine/Dentistry. University of Murcia. 30100 Espinardo. Murcia. Spain.

ABSTRACT

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001). However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

Show MeSH
Related in: MedlinePlus