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Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

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(a–b) Phylum-level changes in different season-site assemblages of prokaryote and eukaryote sequences. The phylum level distribution presented is based on the 80% similarity clusters of OTUs.
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f3: (a–b) Phylum-level changes in different season-site assemblages of prokaryote and eukaryote sequences. The phylum level distribution presented is based on the 80% similarity clusters of OTUs.

Mentions: Shifts in the proportions of sequences for different bacteria phyla are shown in Fig. 3a. In general, different samples showed a relative similar trend in terms of richness as well as indicated by Chao1 and Ace indexes during the same season (S2a). The composition and biomass of taxa exhibited a remarkable spatial and temporal heterogeneity with a pronounced longitudinal gradient. Cyanophyta, Proteobacteria, Actinobacteria, and Bacteroidetes were always highest across all water samples, with more than 80% proportion. However, Sites 0 and 2 exhibited greatly decreased relative abundance of Proteobacteria in summer and autumn, as shown in green type. Except for autumn, the proportion of Cyanophyta in Sites 0 and 2 were well below those of other sites. Firmicutes proportions were highest in the summer samples. Changes in the proportion of sequences of different fungal phyla are shown in Fig. 3b, and richness in Apring and Summer significantly higher than others (S2b). Interestingly, except for the winter samples, Metazoa had the highest abundance among the samples. Cryptomonadales always existed in all samples, and were highest in number in spring and autumn. Cercozoa were most plentiful during winter, particularly in Sites 3, 8, and 13, accounting for more than 60% in those sites, such a high percentage was associated with low frequency during winter sampling. Chlorophyta had significantly stronger samples in summer than others, similar to Diatomea.


Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

(a–b) Phylum-level changes in different season-site assemblages of prokaryote and eukaryote sequences. The phylum level distribution presented is based on the 80% similarity clusters of OTUs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225533&req=5

f3: (a–b) Phylum-level changes in different season-site assemblages of prokaryote and eukaryote sequences. The phylum level distribution presented is based on the 80% similarity clusters of OTUs.
Mentions: Shifts in the proportions of sequences for different bacteria phyla are shown in Fig. 3a. In general, different samples showed a relative similar trend in terms of richness as well as indicated by Chao1 and Ace indexes during the same season (S2a). The composition and biomass of taxa exhibited a remarkable spatial and temporal heterogeneity with a pronounced longitudinal gradient. Cyanophyta, Proteobacteria, Actinobacteria, and Bacteroidetes were always highest across all water samples, with more than 80% proportion. However, Sites 0 and 2 exhibited greatly decreased relative abundance of Proteobacteria in summer and autumn, as shown in green type. Except for autumn, the proportion of Cyanophyta in Sites 0 and 2 were well below those of other sites. Firmicutes proportions were highest in the summer samples. Changes in the proportion of sequences of different fungal phyla are shown in Fig. 3b, and richness in Apring and Summer significantly higher than others (S2b). Interestingly, except for the winter samples, Metazoa had the highest abundance among the samples. Cryptomonadales always existed in all samples, and were highest in number in spring and autumn. Cercozoa were most plentiful during winter, particularly in Sites 3, 8, and 13, accounting for more than 60% in those sites, such a high percentage was associated with low frequency during winter sampling. Chlorophyta had significantly stronger samples in summer than others, similar to Diatomea.

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

Show MeSH