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Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

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(a–b) Different taxonomic numbers of sequence and derived OTU in prokaryote and eukaryote.
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f2: (a–b) Different taxonomic numbers of sequence and derived OTU in prokaryote and eukaryote.

Mentions: A total of 312,949 16S rRNA sequences and 374,752 18S rRNA sequences were selected for classification. Dominant length distributions were approximately 429 and 420 bp, respectively. The taxonomic distributions of the 16S rRNA sequences obtained in the water samples are shown in Fig. 2a. The majority of bacteria sequences (105,673 sequences) belonged to Cyanophyta. The second largest group was Actinobacteria (49,298), followed by Firmicutes (40,013). Betaproteobacteria and Alphaproteobacteria were the most abundant Proteobacteria, making up approximately 56% and 30%, respectively, of the Proteobacteria sequences (43,091). Based on the 97% species similarity, a total of 1,652 OTUs derived from 280,382 sequences were detected in this study. Among these OTUs, 309 were Bacteroidetes OTUs (18.7%), 233 were Cyanophyta (14.1%), and 198 were Alphaproteobacteria (12.0%). The taxonomic distributions of the 18S rRNA sequences obtained in the water samples are shown in Fig. 2b. The majority of fungal sequences (129,819 sequences) belonged to Metazoa. The second largest group was Cryptomonadales (72,845), followed by Chlorophyta (30,194). Eukaryotic algae included Cryptomonadales, Chlorophyta, Diatomea, Dinoflagellata, Kathablepharidae, Chrysophyceae, Glaucophyta, Stramenopiles, and Prymnesiophyceae (126,631 sequences, accounting for 36.4% of total sequences). Similarly, a total of 1,182 OTUs derived from 357,732 fungal sequences were detected in this study. Among these OTUs, 177 were Cercozoa OTUs (15.0%), 129 were Chlorophyta (11.0%), 93 were Metazoa (7.9%), 53 were Cryptomonadales (4.5%), and 27 were Diatomea (2.3%).


Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

(a–b) Different taxonomic numbers of sequence and derived OTU in prokaryote and eukaryote.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225533&req=5

f2: (a–b) Different taxonomic numbers of sequence and derived OTU in prokaryote and eukaryote.
Mentions: A total of 312,949 16S rRNA sequences and 374,752 18S rRNA sequences were selected for classification. Dominant length distributions were approximately 429 and 420 bp, respectively. The taxonomic distributions of the 16S rRNA sequences obtained in the water samples are shown in Fig. 2a. The majority of bacteria sequences (105,673 sequences) belonged to Cyanophyta. The second largest group was Actinobacteria (49,298), followed by Firmicutes (40,013). Betaproteobacteria and Alphaproteobacteria were the most abundant Proteobacteria, making up approximately 56% and 30%, respectively, of the Proteobacteria sequences (43,091). Based on the 97% species similarity, a total of 1,652 OTUs derived from 280,382 sequences were detected in this study. Among these OTUs, 309 were Bacteroidetes OTUs (18.7%), 233 were Cyanophyta (14.1%), and 198 were Alphaproteobacteria (12.0%). The taxonomic distributions of the 18S rRNA sequences obtained in the water samples are shown in Fig. 2b. The majority of fungal sequences (129,819 sequences) belonged to Metazoa. The second largest group was Cryptomonadales (72,845), followed by Chlorophyta (30,194). Eukaryotic algae included Cryptomonadales, Chlorophyta, Diatomea, Dinoflagellata, Kathablepharidae, Chrysophyceae, Glaucophyta, Stramenopiles, and Prymnesiophyceae (126,631 sequences, accounting for 36.4% of total sequences). Similarly, a total of 1,182 OTUs derived from 357,732 fungal sequences were detected in this study. Among these OTUs, 177 were Cercozoa OTUs (15.0%), 129 were Chlorophyta (11.0%), 93 were Metazoa (7.9%), 53 were Cryptomonadales (4.5%), and 27 were Diatomea (2.3%).

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

Show MeSH