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Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

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Locations and sampling sites in estuary reservoir.Six monitoring positions were marked as 0, 2, 3, 8, 13, 15 respectively. Satellite image was obtained by synthesizing the data from Satellite Environment Center, Ministry of Environmental Protection in China in free of charge in website of [http://www.secmep.cn/secPortal/portal/column/itemDetails.faces?itemid=8ae67f88272ce22801272d0f50410005] using commercial GeoStar3.0 (http://www.geostar.com.cn) and free Gimp 2.8.10 software (http://www.gimp.org/).
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f1: Locations and sampling sites in estuary reservoir.Six monitoring positions were marked as 0, 2, 3, 8, 13, 15 respectively. Satellite image was obtained by synthesizing the data from Satellite Environment Center, Ministry of Environmental Protection in China in free of charge in website of [http://www.secmep.cn/secPortal/portal/column/itemDetails.faces?itemid=8ae67f88272ce22801272d0f50410005] using commercial GeoStar3.0 (http://www.geostar.com.cn) and free Gimp 2.8.10 software (http://www.gimp.org/).

Mentions: Supporting Information (SI) Table S1 lists the environmental parameters, including water temperature, dissolved oxygen (DO), permanganate index (CODMn), total nitrogen (TN), total phosphorus (TP), transparency, and chlorophyll-a, microcystin-RR (MC_RR) and microcystin-LR (MC_LR). Transparency increased whereas TP decreased from the head to the tail of the reservoir (Fig. 1). CODMn and chlorophyll-a concentrations were higher in Site 3 than in Site 2 (Fig. 1). The chlorophyll-a of Site 0 was always lower than those of other sites and dramatically influenced by air temperature as well as CODMn, particularly by air temperatures ranging from 22°C to 30°C, and water quality that meets the current national “Sanitary standard for drinking water” (GB5749-2006). Water temperature varied more significantly in periods. Irregular changes in TN were observed in the six sites. The DO of all samples were relatively stable at approximately 9.11 mg/l. MC_RR and MC_LR were lower than 0.5 μg/l in most samples, with only the MC_LR in sample 0410-3 reaching 0.77 μg/l, which was still lower than the national standard of 1 μg/l.


Community dynamics of prokaryotic and eukaryotic microbes in an estuary reservoir.

Sun Z, Li G, Wang C, Jing Y, Zhu Y, Zhang S, Liu Y - Sci Rep (2014)

Locations and sampling sites in estuary reservoir.Six monitoring positions were marked as 0, 2, 3, 8, 13, 15 respectively. Satellite image was obtained by synthesizing the data from Satellite Environment Center, Ministry of Environmental Protection in China in free of charge in website of [http://www.secmep.cn/secPortal/portal/column/itemDetails.faces?itemid=8ae67f88272ce22801272d0f50410005] using commercial GeoStar3.0 (http://www.geostar.com.cn) and free Gimp 2.8.10 software (http://www.gimp.org/).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225533&req=5

f1: Locations and sampling sites in estuary reservoir.Six monitoring positions were marked as 0, 2, 3, 8, 13, 15 respectively. Satellite image was obtained by synthesizing the data from Satellite Environment Center, Ministry of Environmental Protection in China in free of charge in website of [http://www.secmep.cn/secPortal/portal/column/itemDetails.faces?itemid=8ae67f88272ce22801272d0f50410005] using commercial GeoStar3.0 (http://www.geostar.com.cn) and free Gimp 2.8.10 software (http://www.gimp.org/).
Mentions: Supporting Information (SI) Table S1 lists the environmental parameters, including water temperature, dissolved oxygen (DO), permanganate index (CODMn), total nitrogen (TN), total phosphorus (TP), transparency, and chlorophyll-a, microcystin-RR (MC_RR) and microcystin-LR (MC_LR). Transparency increased whereas TP decreased from the head to the tail of the reservoir (Fig. 1). CODMn and chlorophyll-a concentrations were higher in Site 3 than in Site 2 (Fig. 1). The chlorophyll-a of Site 0 was always lower than those of other sites and dramatically influenced by air temperature as well as CODMn, particularly by air temperatures ranging from 22°C to 30°C, and water quality that meets the current national “Sanitary standard for drinking water” (GB5749-2006). Water temperature varied more significantly in periods. Irregular changes in TN were observed in the six sites. The DO of all samples were relatively stable at approximately 9.11 mg/l. MC_RR and MC_LR were lower than 0.5 μg/l in most samples, with only the MC_LR in sample 0410-3 reaching 0.77 μg/l, which was still lower than the national standard of 1 μg/l.

Bottom Line: Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level.Air temperature had a stronger effect than sampling location on the microbial community structure.Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, Fudan University, Handan Road, 200433, Shanghai, China.

ABSTRACT
This study demonstrates both prokaryotic and eukaryotic community structures and dominant taxonomies in different positions of the greatest estuary reservoir for drinking water source in the world in four seasons of one year using 454 pyrosequencing method with total of 312,949 16S rRNA and 374,752 18S rRNA gene fragments, including 1,652 bacteria OTUs and 1,182 fungus OTUs. During winter and spring, the community composition at the phylum level showed that microorganisms had similar structures but their quantities were different. Similarly, obvious changes at the genus level were observed among the samples taken in winter and spring between summer and fall. Microorganisms located the reservoir inlet were founded to be different from those in rear at both phylum and genus level. Air temperature had a stronger effect than sampling location on the microbial community structure. Total nitrogen and dissolved oxygen were algae-monitoring indicators during the whole year. Moreover, Bacillus was an efficient indicator during summer and autumn for bacteria OTUs.

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