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One case of swine hepatitis E virus and porcine reproductive and respiratory syndrome virus co-infection in weaned pigs.

Mao J, Zhao Y, She R, Xiao P, Tian J, Chen J - Virol. J. (2013)

Bottom Line: We found that this sample was positive for the presence of HEV and PRRSV.Severe pathologic changes were observed.We speculated that co-infection with PRRSV and HEV might lead to more serious problems.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Veterinary Pathology, Key Laboratory of Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing 100193, China. sheruiping@126.com.

ABSTRACT

Background: Using various methods, we analyzed the cause of death among weaned pigs from a pig farm in Hebei Province, China. All 300 piglets (100% fatality) were identified as moribund, with death occurring within 1 month from the onset of clinical signs.

Results: A single case exhibited obvious hemorrhagic necrotic changes with massive lymphocytic infiltration in multiple organs, in particular the liver, lungs and intestines. Dysplasia and lymphocyte deterioration were common in lymphatic organs. No visible bacterial colonies from liver and spleen were observed in nutrient, MacConkey, and blood agar plates. Using polymerase chain reaction techniques for this case, we attempted to detect a number of epidemic swine viruses in spleen and liver, including PRRSV, CSF, HEV, and PCV2. We found that this sample was positive for the presence of HEV and PRRSV.

Conclusions: We have detected HEV and PRRSV co-infection in one piglet. Severe pathologic changes were observed. The high mortality of weaned pigs which showed the similar clinical syptom was possibly a result of HEV and PRRSV co-infection, which has rarely been reported previously. We speculated that co-infection with PRRSV and HEV might lead to more serious problems.

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Phylogenetic analysis based on ORF2 (348 nt) depicting the genetic relationship between our isolate from this study and isolates from across China or from other countries. A neighbor-joining tree was constructed with bootstrap values calculated from 1,000 replicates. Isolates used for comparative analysis were Burma (GenBank Accession No. M73218), Myanmar (D10330), Pakistan (AF185822), Madras (X99441), HEV 037 (X98292), SAR55(M80581), Ugih (D11092), Eygypt (AF051352), Mexico (M74506), CHN-XJ-SW13 (GU119961), TW6196E(HQ634346), HEV-T1 (AJ272108), swCH25 (AY594199), swJR-P5 (AB481229), US2 (AF060669), US1 (AF060668), and Avian HEV (AY535004).
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Figure 4: Phylogenetic analysis based on ORF2 (348 nt) depicting the genetic relationship between our isolate from this study and isolates from across China or from other countries. A neighbor-joining tree was constructed with bootstrap values calculated from 1,000 replicates. Isolates used for comparative analysis were Burma (GenBank Accession No. M73218), Myanmar (D10330), Pakistan (AF185822), Madras (X99441), HEV 037 (X98292), SAR55(M80581), Ugih (D11092), Eygypt (AF051352), Mexico (M74506), CHN-XJ-SW13 (GU119961), TW6196E(HQ634346), HEV-T1 (AJ272108), swCH25 (AY594199), swJR-P5 (AB481229), US2 (AF060669), US1 (AF060668), and Avian HEV (AY535004).

Mentions: Using nutrient, MacConkey, and blood agar plates, there were no visible bacterial colonies. Antibiotic therapy did not affect morbidity or mortality rates. We used polymerase chain reaction (PCR) techniques to determine the presence of viruses (Table 1). We detected the presence of PRRSV, HEV, CSFV, and PCV2. PRRSV and HEV were detected in liver tissues, while liver and lung tissues were negative for CSFV and PCV2 (Figure 2) [10]. The Hebeico1 PRRSV isolate was a North American genotype, and was closely related to the HUB1, HUB2, JXA1, HUN4, GD (Figure 3). The Hebeico2 HEV isolate belonged to genotype 4 (Figure 4).


One case of swine hepatitis E virus and porcine reproductive and respiratory syndrome virus co-infection in weaned pigs.

Mao J, Zhao Y, She R, Xiao P, Tian J, Chen J - Virol. J. (2013)

Phylogenetic analysis based on ORF2 (348 nt) depicting the genetic relationship between our isolate from this study and isolates from across China or from other countries. A neighbor-joining tree was constructed with bootstrap values calculated from 1,000 replicates. Isolates used for comparative analysis were Burma (GenBank Accession No. M73218), Myanmar (D10330), Pakistan (AF185822), Madras (X99441), HEV 037 (X98292), SAR55(M80581), Ugih (D11092), Eygypt (AF051352), Mexico (M74506), CHN-XJ-SW13 (GU119961), TW6196E(HQ634346), HEV-T1 (AJ272108), swCH25 (AY594199), swJR-P5 (AB481229), US2 (AF060669), US1 (AF060668), and Avian HEV (AY535004).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225530&req=5

Figure 4: Phylogenetic analysis based on ORF2 (348 nt) depicting the genetic relationship between our isolate from this study and isolates from across China or from other countries. A neighbor-joining tree was constructed with bootstrap values calculated from 1,000 replicates. Isolates used for comparative analysis were Burma (GenBank Accession No. M73218), Myanmar (D10330), Pakistan (AF185822), Madras (X99441), HEV 037 (X98292), SAR55(M80581), Ugih (D11092), Eygypt (AF051352), Mexico (M74506), CHN-XJ-SW13 (GU119961), TW6196E(HQ634346), HEV-T1 (AJ272108), swCH25 (AY594199), swJR-P5 (AB481229), US2 (AF060669), US1 (AF060668), and Avian HEV (AY535004).
Mentions: Using nutrient, MacConkey, and blood agar plates, there were no visible bacterial colonies. Antibiotic therapy did not affect morbidity or mortality rates. We used polymerase chain reaction (PCR) techniques to determine the presence of viruses (Table 1). We detected the presence of PRRSV, HEV, CSFV, and PCV2. PRRSV and HEV were detected in liver tissues, while liver and lung tissues were negative for CSFV and PCV2 (Figure 2) [10]. The Hebeico1 PRRSV isolate was a North American genotype, and was closely related to the HUB1, HUB2, JXA1, HUN4, GD (Figure 3). The Hebeico2 HEV isolate belonged to genotype 4 (Figure 4).

Bottom Line: We found that this sample was positive for the presence of HEV and PRRSV.Severe pathologic changes were observed.We speculated that co-infection with PRRSV and HEV might lead to more serious problems.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Veterinary Pathology, Key Laboratory of Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing 100193, China. sheruiping@126.com.

ABSTRACT

Background: Using various methods, we analyzed the cause of death among weaned pigs from a pig farm in Hebei Province, China. All 300 piglets (100% fatality) were identified as moribund, with death occurring within 1 month from the onset of clinical signs.

Results: A single case exhibited obvious hemorrhagic necrotic changes with massive lymphocytic infiltration in multiple organs, in particular the liver, lungs and intestines. Dysplasia and lymphocyte deterioration were common in lymphatic organs. No visible bacterial colonies from liver and spleen were observed in nutrient, MacConkey, and blood agar plates. Using polymerase chain reaction techniques for this case, we attempted to detect a number of epidemic swine viruses in spleen and liver, including PRRSV, CSF, HEV, and PCV2. We found that this sample was positive for the presence of HEV and PRRSV.

Conclusions: We have detected HEV and PRRSV co-infection in one piglet. Severe pathologic changes were observed. The high mortality of weaned pigs which showed the similar clinical syptom was possibly a result of HEV and PRRSV co-infection, which has rarely been reported previously. We speculated that co-infection with PRRSV and HEV might lead to more serious problems.

Show MeSH
Related in: MedlinePlus