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Effects of dehydration and blockade of angiotensin II AT1 receptor on stress hormones and anti-oxidants in the one-humped camel.

Ali MA, Kazzam E, Amir N, Nyberg F, Adem A - BMC Vet. Res. (2013)

Bottom Line: Plasma levels of norepinephrine and dopamine were significantly increased (P < 0.01) in both treated and dehydrated groups compared to time matched control, whereas Plasma epinephrine level showed significant decrease (P < 0.05) in both treated and dehydrated groups compared to control.Plasma cortisol also showed significant increase (P < 0.01) in both treated and dehydrated groups compared to control.In conclusion, the results of this study demonstrated that dehydration substantially increased the circulating levels of norepinephrine, dopamine and cortisol but decreased plasma epinephrine.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine and Health Sciences, United Arab Emirates University, P,O, Box 17666, Al Ain, United Arab Emirates. abdu.adem@uaeu.ac.ae.

ABSTRACT

Background: The objective of this study was to provide for the first time data on plasma catecholamines, cortisol, glutathione and malondialdehyde after long term dehydration (20 days) in the presence and absence of angiotensin II (Ang II) AT1 receptor blocker (losartan) versus levels in time-matched, non-dehydrated control camels and to record the responses of glutathione and malondialdehyde activity in liver and kidney homogenates in control, dehydrated-losartan treated and dehydrated camels. Eighteen male camels were studied, six hydrated (control group), six dehydrated and treated with losartan (treated group) and six dehydrated not treated (dehydrated).

Results: Plasma levels of norepinephrine and dopamine were significantly increased (P < 0.01) in both treated and dehydrated groups compared to time matched control, whereas Plasma epinephrine level showed significant decrease (P < 0.05) in both treated and dehydrated groups compared to control. Plasma cortisol also showed significant increase (P < 0.01) in both treated and dehydrated groups compared to control. Glutathione levels in plasma, liver and kidney homogenates for both treated and dehydrated groups reveled significant increase (P < 0.05) Likewise, malondialdehyde levels in plasma, liver and kidney homogenates were substantially and significantly increased in both treated and dehydrated groups.

Conclusion: In conclusion, the results of this study demonstrated that dehydration substantially increased the circulating levels of norepinephrine, dopamine and cortisol but decreased plasma epinephrine. Similarly, losartan showed similar effects to that of dehydration. In addition, this investigation showed dehydration alone or in combination with losartan induced significant increments in glutathione and malondialdehyde activities in plasma, liver and kidney homogenates, presumably in order to counteract the potentially damaging effects of free radicals. Blockade of angiotensin II AT1 receptors did not alter significantly the response of dehydration in any of these indices.

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Malondialdehyde activities (μM/ml) in plasma, liver and kidney homogenates of control, treated and dehydrated camels. Data are mean ± SEM. Asterisk denotes significance from control (* P < 0.05, ** P < 0.01).
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Figure 4: Malondialdehyde activities (μM/ml) in plasma, liver and kidney homogenates of control, treated and dehydrated camels. Data are mean ± SEM. Asterisk denotes significance from control (* P < 0.05, ** P < 0.01).

Mentions: Plasma malondialdehyde level in control camels was 4.95 ± 0.56 μm/l. The level in both dehydrated groups increased significantly (P < 0.001) after 20 days of water deprivation in presence of losartan (12.58 ± 1.07 um/l) and in absence of losartan (12.23 + 1.07 um/l) compared to control camels (Figure 4). MDA level in liver homogenate in control was 3.46 ± 0.56 μm/l. In the treated the level was doubled (7.33 ± 1.83 μm/l) (but this result was from one camel only and was not used for comparison and statistical significance). On the other hand, the dehydrated group liver homogenate showed significant (P < 0.01) increase, reaching (10 folds compared to control). Results from kidney homogenate for control was 3.02 ±0.26 μm/l and for the treated was 7.04 ± 0.88 μm/l, while for the dehydrated was 5.35 ± 0.91 μm/l (Figure 4).


Effects of dehydration and blockade of angiotensin II AT1 receptor on stress hormones and anti-oxidants in the one-humped camel.

Ali MA, Kazzam E, Amir N, Nyberg F, Adem A - BMC Vet. Res. (2013)

Malondialdehyde activities (μM/ml) in plasma, liver and kidney homogenates of control, treated and dehydrated camels. Data are mean ± SEM. Asterisk denotes significance from control (* P < 0.05, ** P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225509&req=5

Figure 4: Malondialdehyde activities (μM/ml) in plasma, liver and kidney homogenates of control, treated and dehydrated camels. Data are mean ± SEM. Asterisk denotes significance from control (* P < 0.05, ** P < 0.01).
Mentions: Plasma malondialdehyde level in control camels was 4.95 ± 0.56 μm/l. The level in both dehydrated groups increased significantly (P < 0.001) after 20 days of water deprivation in presence of losartan (12.58 ± 1.07 um/l) and in absence of losartan (12.23 + 1.07 um/l) compared to control camels (Figure 4). MDA level in liver homogenate in control was 3.46 ± 0.56 μm/l. In the treated the level was doubled (7.33 ± 1.83 μm/l) (but this result was from one camel only and was not used for comparison and statistical significance). On the other hand, the dehydrated group liver homogenate showed significant (P < 0.01) increase, reaching (10 folds compared to control). Results from kidney homogenate for control was 3.02 ±0.26 μm/l and for the treated was 7.04 ± 0.88 μm/l, while for the dehydrated was 5.35 ± 0.91 μm/l (Figure 4).

Bottom Line: Plasma levels of norepinephrine and dopamine were significantly increased (P < 0.01) in both treated and dehydrated groups compared to time matched control, whereas Plasma epinephrine level showed significant decrease (P < 0.05) in both treated and dehydrated groups compared to control.Plasma cortisol also showed significant increase (P < 0.01) in both treated and dehydrated groups compared to control.In conclusion, the results of this study demonstrated that dehydration substantially increased the circulating levels of norepinephrine, dopamine and cortisol but decreased plasma epinephrine.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine and Health Sciences, United Arab Emirates University, P,O, Box 17666, Al Ain, United Arab Emirates. abdu.adem@uaeu.ac.ae.

ABSTRACT

Background: The objective of this study was to provide for the first time data on plasma catecholamines, cortisol, glutathione and malondialdehyde after long term dehydration (20 days) in the presence and absence of angiotensin II (Ang II) AT1 receptor blocker (losartan) versus levels in time-matched, non-dehydrated control camels and to record the responses of glutathione and malondialdehyde activity in liver and kidney homogenates in control, dehydrated-losartan treated and dehydrated camels. Eighteen male camels were studied, six hydrated (control group), six dehydrated and treated with losartan (treated group) and six dehydrated not treated (dehydrated).

Results: Plasma levels of norepinephrine and dopamine were significantly increased (P < 0.01) in both treated and dehydrated groups compared to time matched control, whereas Plasma epinephrine level showed significant decrease (P < 0.05) in both treated and dehydrated groups compared to control. Plasma cortisol also showed significant increase (P < 0.01) in both treated and dehydrated groups compared to control. Glutathione levels in plasma, liver and kidney homogenates for both treated and dehydrated groups reveled significant increase (P < 0.05) Likewise, malondialdehyde levels in plasma, liver and kidney homogenates were substantially and significantly increased in both treated and dehydrated groups.

Conclusion: In conclusion, the results of this study demonstrated that dehydration substantially increased the circulating levels of norepinephrine, dopamine and cortisol but decreased plasma epinephrine. Similarly, losartan showed similar effects to that of dehydration. In addition, this investigation showed dehydration alone or in combination with losartan induced significant increments in glutathione and malondialdehyde activities in plasma, liver and kidney homogenates, presumably in order to counteract the potentially damaging effects of free radicals. Blockade of angiotensin II AT1 receptors did not alter significantly the response of dehydration in any of these indices.

Show MeSH
Related in: MedlinePlus