Characterization of ASKP1240, a fully human antibody targeting human CD40 with potent immunosuppressive effects.
Bottom Line: In addition, ASKP1240 did not destabilize platelet thrombi under physiological high shear conditions while mouse anti-human CD154 mAb (mu5C8) did.And ASKP1240 itself did not activate platelet and endothelial cells.The immunosuppressive effect was well correlated with the CD40 receptor saturation.
Affiliation: Development Research Laboratories, Kyowa Hakko Kirin Co., Ltd., Shizuoka, Japan.Show MeSH
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Mentions: Since CD40 ligation can lead to internalization of cell surface CD40 32, a determination was made as to whether ASKP1240 can elicit internalization of CD40. In this experiment, internalized antigen showed FITC fluorescence only, but residual cell surface antigen showed both PE and FITC fluorescence. Although binding of the anti-CD40 agonistic mAb, G28.5, leads to an immediate decrease in PE fluorescence, ASKP1240 affected neither FITC nor PE fluorescence levels (Figure 2).
Affiliation: Development Research Laboratories, Kyowa Hakko Kirin Co., Ltd., Shizuoka, Japan.