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Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.

Diesing AK, Nossol C, Faber-Zuschratter H, Zuschratter W, Renner L, Sokolova O, Naumann M, Rothkötter HJ - Anat Rec (Hoboken) (2013)

Bottom Line: The aim of the present study was to analyze the initial host contact to the bacteria.Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli.Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.

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Transmission electron microscopy of the same monolayer as shown in Fig. 3A–C: (A) Transmission electron micrographs of human gastric epithelial NCI-N87 cells exposed to H. pylori for 1 hr, bacteria are dark or pale contrasted–bar 1 µm. (B) H. pylori bacteria are closely associated to the microvilli of epithelial NCI-N87 cells, however, no specific cell contact structure is established–bar 1 µm. (C) Bacterium is located along the microvilli of one epithelial cell, compare to Fig. 3C–bar 1 µm. (D) Host cell microvilli of two neighboring cells surround bacteria after 1 hr of interaction–bar 1 µm.
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fig04: Transmission electron microscopy of the same monolayer as shown in Fig. 3A–C: (A) Transmission electron micrographs of human gastric epithelial NCI-N87 cells exposed to H. pylori for 1 hr, bacteria are dark or pale contrasted–bar 1 µm. (B) H. pylori bacteria are closely associated to the microvilli of epithelial NCI-N87 cells, however, no specific cell contact structure is established–bar 1 µm. (C) Bacterium is located along the microvilli of one epithelial cell, compare to Fig. 3C–bar 1 µm. (D) Host cell microvilli of two neighboring cells surround bacteria after 1 hr of interaction–bar 1 µm.

Mentions: H. pylori bacteria were added for 1 hr to the apical side of these NCI-N87 monolayers. After this short period a close interaction of the bacteria cell wall with epithelial microvilli was detected using scanning electron microscopy (Fig. 3A–C). The microvilli were in part thicker at the interacting site and some budding was observed. Comparable to non-exposed NCI-N87 monolayers (see Fig. 2C), here also a marked variation of microvillus length and density per area was observed. In addition, the host/bacteria interface was studied by transmission electron microscopy (Fig. 4). However, there were no specific ultrastructural differentiations of the microvilli, bacteria located in the area of two neighboring cells were often entrapped by several microvilli (Fig. 4A–D).


Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.

Diesing AK, Nossol C, Faber-Zuschratter H, Zuschratter W, Renner L, Sokolova O, Naumann M, Rothkötter HJ - Anat Rec (Hoboken) (2013)

Transmission electron microscopy of the same monolayer as shown in Fig. 3A–C: (A) Transmission electron micrographs of human gastric epithelial NCI-N87 cells exposed to H. pylori for 1 hr, bacteria are dark or pale contrasted–bar 1 µm. (B) H. pylori bacteria are closely associated to the microvilli of epithelial NCI-N87 cells, however, no specific cell contact structure is established–bar 1 µm. (C) Bacterium is located along the microvilli of one epithelial cell, compare to Fig. 3C–bar 1 µm. (D) Host cell microvilli of two neighboring cells surround bacteria after 1 hr of interaction–bar 1 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225472&req=5

fig04: Transmission electron microscopy of the same monolayer as shown in Fig. 3A–C: (A) Transmission electron micrographs of human gastric epithelial NCI-N87 cells exposed to H. pylori for 1 hr, bacteria are dark or pale contrasted–bar 1 µm. (B) H. pylori bacteria are closely associated to the microvilli of epithelial NCI-N87 cells, however, no specific cell contact structure is established–bar 1 µm. (C) Bacterium is located along the microvilli of one epithelial cell, compare to Fig. 3C–bar 1 µm. (D) Host cell microvilli of two neighboring cells surround bacteria after 1 hr of interaction–bar 1 µm.
Mentions: H. pylori bacteria were added for 1 hr to the apical side of these NCI-N87 monolayers. After this short period a close interaction of the bacteria cell wall with epithelial microvilli was detected using scanning electron microscopy (Fig. 3A–C). The microvilli were in part thicker at the interacting site and some budding was observed. Comparable to non-exposed NCI-N87 monolayers (see Fig. 2C), here also a marked variation of microvillus length and density per area was observed. In addition, the host/bacteria interface was studied by transmission electron microscopy (Fig. 4). However, there were no specific ultrastructural differentiations of the microvilli, bacteria located in the area of two neighboring cells were often entrapped by several microvilli (Fig. 4A–D).

Bottom Line: The aim of the present study was to analyze the initial host contact to the bacteria.Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli.Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.

Show MeSH
Related in: MedlinePlus