Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.
Bottom Line: The aim of the present study was to analyze the initial host contact to the bacteria.Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli.Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria.
Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.Show MeSH
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Mentions: H. pylori bacteria were added for 1 hr to the apical side of these NCI-N87 monolayers. After this short period a close interaction of the bacteria cell wall with epithelial microvilli was detected using scanning electron microscopy (Fig. 3A–C). The microvilli were in part thicker at the interacting site and some budding was observed. Comparable to non-exposed NCI-N87 monolayers (see Fig. 2C), here also a marked variation of microvillus length and density per area was observed. In addition, the host/bacteria interface was studied by transmission electron microscopy (Fig. 4). However, there were no specific ultrastructural differentiations of the microvilli, bacteria located in the area of two neighboring cells were often entrapped by several microvilli (Fig. 4A–D).
Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.