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Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.

Diesing AK, Nossol C, Faber-Zuschratter H, Zuschratter W, Renner L, Sokolova O, Naumann M, Rothkötter HJ - Anat Rec (Hoboken) (2013)

Bottom Line: The aim of the present study was to analyze the initial host contact to the bacteria.Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli.Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.

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Related in: MedlinePlus

Scanning electron microscopy of an epithelial monolayer of NCI-N87 cells, apical view, 1 hr after the exposition with bacteria (MOI 50:1). H. pylori are located on the epithelium, within this short period close attachments of microvilli/bacteria have been established. (A) overview—bar 1 µm, (B) budding of microvilli toward the bacteria cell wall—bar 200 nm, (C) some bacteria have few if any microvillus attachments—bar 250 nm.
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fig03: Scanning electron microscopy of an epithelial monolayer of NCI-N87 cells, apical view, 1 hr after the exposition with bacteria (MOI 50:1). H. pylori are located on the epithelium, within this short period close attachments of microvilli/bacteria have been established. (A) overview—bar 1 µm, (B) budding of microvilli toward the bacteria cell wall—bar 200 nm, (C) some bacteria have few if any microvillus attachments—bar 250 nm.

Mentions: H. pylori bacteria were added for 1 hr to the apical side of these NCI-N87 monolayers. After this short period a close interaction of the bacteria cell wall with epithelial microvilli was detected using scanning electron microscopy (Fig. 3A–C). The microvilli were in part thicker at the interacting site and some budding was observed. Comparable to non-exposed NCI-N87 monolayers (see Fig. 2C), here also a marked variation of microvillus length and density per area was observed. In addition, the host/bacteria interface was studied by transmission electron microscopy (Fig. 4). However, there were no specific ultrastructural differentiations of the microvilli, bacteria located in the area of two neighboring cells were often entrapped by several microvilli (Fig. 4A–D).


Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.

Diesing AK, Nossol C, Faber-Zuschratter H, Zuschratter W, Renner L, Sokolova O, Naumann M, Rothkötter HJ - Anat Rec (Hoboken) (2013)

Scanning electron microscopy of an epithelial monolayer of NCI-N87 cells, apical view, 1 hr after the exposition with bacteria (MOI 50:1). H. pylori are located on the epithelium, within this short period close attachments of microvilli/bacteria have been established. (A) overview—bar 1 µm, (B) budding of microvilli toward the bacteria cell wall—bar 200 nm, (C) some bacteria have few if any microvillus attachments—bar 250 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225472&req=5

fig03: Scanning electron microscopy of an epithelial monolayer of NCI-N87 cells, apical view, 1 hr after the exposition with bacteria (MOI 50:1). H. pylori are located on the epithelium, within this short period close attachments of microvilli/bacteria have been established. (A) overview—bar 1 µm, (B) budding of microvilli toward the bacteria cell wall—bar 200 nm, (C) some bacteria have few if any microvillus attachments—bar 250 nm.
Mentions: H. pylori bacteria were added for 1 hr to the apical side of these NCI-N87 monolayers. After this short period a close interaction of the bacteria cell wall with epithelial microvilli was detected using scanning electron microscopy (Fig. 3A–C). The microvilli were in part thicker at the interacting site and some budding was observed. Comparable to non-exposed NCI-N87 monolayers (see Fig. 2C), here also a marked variation of microvillus length and density per area was observed. In addition, the host/bacteria interface was studied by transmission electron microscopy (Fig. 4). However, there were no specific ultrastructural differentiations of the microvilli, bacteria located in the area of two neighboring cells were often entrapped by several microvilli (Fig. 4A–D).

Bottom Line: The aim of the present study was to analyze the initial host contact to the bacteria.Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli.Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.

Show MeSH
Related in: MedlinePlus