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Effects of coenzyme Q10 on the ratio of TH1/TH2 in experimental autoimmune encephalomyelitis model of multiple sclerosis in C57BL/6.

Soleimani M, Jameie SB, Barati M, Mehdizadeh M, Kerdari M - Iran. Biomed. J. (2014)

Bottom Line: Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS.Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones.The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Jameie.SB@iums.ac.ir.

ABSTRACT

Background: Multiple sclerosis (MS) is known as a progressive central nervous system inflammatory disease. Certain factors, such as interleukins, inflammatory cells, and oxidative stress are supposed to involve in MS etiology. Because of the important role of oxidative stress, antioxidant therapy for MS has received more attention. Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS. Therefore, the present research was designed.

Methods: C57BL/6 female adult mice (n = 30) were used in this study. The animals were randomly divided into trial and control groups. To induce MS, routine procedure for experimental autoimmune encephalomyelitis (EAE) was used, and scoring was performed based on clinical signs. By detecting score one, CoQ10 administration was started (10 mg/kg/three weeks). By using ELISA and real-time PCR, the brain levels of TNF-, IL-10, IL-4, and IL-12 were studied. Statistical tests were used to analyze the data and the P value less than 0.05 was considered to be significant.

Results: Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones. In addition, the level of the TNF- was significantly decreased following CoQ10 administration versus IL-10. The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01).

Conclusion: Our findings showed that CoQ10 is capable of suppressing the inflammatory pathway of MS.

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Related in: MedlinePlus

The expression of mRNA. Using REST software, the fold changes were increased in inflammatory genes of EAE mice as compared to anti-inflammatory genes in this group (*P<0.0.5). BC, brain control
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Figure 5: The expression of mRNA. Using REST software, the fold changes were increased in inflammatory genes of EAE mice as compared to anti-inflammatory genes in this group (*P<0.0.5). BC, brain control

Mentions: Cytokine gene expression. Pro-inflammatory cytokine expression was analyzed using REST software (Qiagen, Germany) (Fig. 5). IL-12 mRNA and TNF- mRNA expression were increased remarkably in EAE model. There was no significant increase of IL-4 in anti-inflammatory cytokine mRNA expression in EAE as compared to control group. Although high increased expression of IL-10 mRNA did not occur in EAE animals, noticeable decline but not significant of IL-10 mRNA expression was seen in sesame and CoQ10 treatment groups. IL-4 mRNA expression in sesame oil group was obviously higher than control group, but IL-10 mRNA expression was obviously expressed lower in EAE mice. Also, a significant lower expression of TNF- mRNA was observed in animals treated with CoQ10 and sesame oils (Table 3).


Effects of coenzyme Q10 on the ratio of TH1/TH2 in experimental autoimmune encephalomyelitis model of multiple sclerosis in C57BL/6.

Soleimani M, Jameie SB, Barati M, Mehdizadeh M, Kerdari M - Iran. Biomed. J. (2014)

The expression of mRNA. Using REST software, the fold changes were increased in inflammatory genes of EAE mice as compared to anti-inflammatory genes in this group (*P<0.0.5). BC, brain control
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4225059&req=5

Figure 5: The expression of mRNA. Using REST software, the fold changes were increased in inflammatory genes of EAE mice as compared to anti-inflammatory genes in this group (*P<0.0.5). BC, brain control
Mentions: Cytokine gene expression. Pro-inflammatory cytokine expression was analyzed using REST software (Qiagen, Germany) (Fig. 5). IL-12 mRNA and TNF- mRNA expression were increased remarkably in EAE model. There was no significant increase of IL-4 in anti-inflammatory cytokine mRNA expression in EAE as compared to control group. Although high increased expression of IL-10 mRNA did not occur in EAE animals, noticeable decline but not significant of IL-10 mRNA expression was seen in sesame and CoQ10 treatment groups. IL-4 mRNA expression in sesame oil group was obviously higher than control group, but IL-10 mRNA expression was obviously expressed lower in EAE mice. Also, a significant lower expression of TNF- mRNA was observed in animals treated with CoQ10 and sesame oils (Table 3).

Bottom Line: Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS.Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones.The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Jameie.SB@iums.ac.ir.

ABSTRACT

Background: Multiple sclerosis (MS) is known as a progressive central nervous system inflammatory disease. Certain factors, such as interleukins, inflammatory cells, and oxidative stress are supposed to involve in MS etiology. Because of the important role of oxidative stress, antioxidant therapy for MS has received more attention. Although coenzyme Q10 (CoQ10) acts as an antioxidant, there is a lack of enough research on its effects on MS. Therefore, the present research was designed.

Methods: C57BL/6 female adult mice (n = 30) were used in this study. The animals were randomly divided into trial and control groups. To induce MS, routine procedure for experimental autoimmune encephalomyelitis (EAE) was used, and scoring was performed based on clinical signs. By detecting score one, CoQ10 administration was started (10 mg/kg/three weeks). By using ELISA and real-time PCR, the brain levels of TNF-, IL-10, IL-4, and IL-12 were studied. Statistical tests were used to analyze the data and the P value less than 0.05 was considered to be significant.

Results: Clinical symptoms in EAE animals were significantly decreased (P<0.05) as compared to control ones. In addition, the level of the TNF- was significantly decreased following CoQ10 administration versus IL-10. The ratio of TH1/TH2 interleukins in treated animals was significantly less than that in non-treated animals (P<0.01).

Conclusion: Our findings showed that CoQ10 is capable of suppressing the inflammatory pathway of MS.

Show MeSH
Related in: MedlinePlus