Limits...
Magnetite nanoparticles inhibit tumor growth and upregulate the expression of p53/p16 in Ehrlich solid carcinoma bearing mice.

Bassiony H, Sabet S, Salah El-Din TA, Mohamed MM, El-Ghor AA - PLoS ONE (2014)

Bottom Line: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor.A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor.It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

ABSTRACT

Background: Magnetite nanoparticles (MNPs) have been widely used as contrast agents and have promising approaches in cancer treatment. In the present study we used Ehrlich solid carcinoma (ESC) bearing mice as a model to investigate MNPs antitumor activity, their effect on expression of p53 and p16 genes as an indicator for apoptotic induction in tumor tissues.

Method: MNPs coated with ascorbic acid (size: 25.0±5.0 nm) were synthesized by co-precipitation method and characterized. Ehrlich mice model were treated with MNPs using 60 mg/Kg day by day for 14 injections; intratumorally (IT) or intraperitoneally (IP). Tumor size, pathological changes and iron content in tumor and normal muscle tissues were assessed. We also assessed changes in expression levels of p53 and p16 genes in addition to p53 protein level by immunohistochemistry.

Results: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor. A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor. This increase was accompanied with increase in p53 protein expression. It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

Conclusion: MNPs might be more effective in breast cancer treatment if injected intratumorally to be directed to the tumor tissues.

Show MeSH

Related in: MedlinePlus

Antitumor effect of MNPs administration on Ehrlich solid tumor growth in mice.Ehrlich tumor bearing mice untreated with MNPs (♦), Ehrlich tumor bearing mice treated IP with MNPs (▪) and Ehrlich tumor bearing mice treated IT with MNPs (▴). Each point represents the mean±SE. n = 6. Representative images of tumor were shown for each group. Images were at the same magnification level.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4222961&req=5

pone-0111960-g006: Antitumor effect of MNPs administration on Ehrlich solid tumor growth in mice.Ehrlich tumor bearing mice untreated with MNPs (♦), Ehrlich tumor bearing mice treated IP with MNPs (▪) and Ehrlich tumor bearing mice treated IT with MNPs (▴). Each point represents the mean±SE. n = 6. Representative images of tumor were shown for each group. Images were at the same magnification level.

Mentions: To assess the anti-tumor effect of MNPs; MNPs were injected IP and IT to Ehrlich tumor bearing mice, and tumor growth rate was measured for up to 4 weeks (Fig. 6). From day 14 to day 21, the tumor size of both MNPs treated groups was similar to that of untreated group without significant difference. After that, an observed significant reduction in tumor growth of IT treated mice (group 6) and less reduction in tumor size was observed in IP treated mice (group 5), while untreated mice showed continuous tumor growth (group 4). The growth inhibition of solid Ehrlich tumor was found to be 47.35% and 37.1% for IT and IP treated animals, respectively.


Magnetite nanoparticles inhibit tumor growth and upregulate the expression of p53/p16 in Ehrlich solid carcinoma bearing mice.

Bassiony H, Sabet S, Salah El-Din TA, Mohamed MM, El-Ghor AA - PLoS ONE (2014)

Antitumor effect of MNPs administration on Ehrlich solid tumor growth in mice.Ehrlich tumor bearing mice untreated with MNPs (♦), Ehrlich tumor bearing mice treated IP with MNPs (▪) and Ehrlich tumor bearing mice treated IT with MNPs (▴). Each point represents the mean±SE. n = 6. Representative images of tumor were shown for each group. Images were at the same magnification level.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222961&req=5

pone-0111960-g006: Antitumor effect of MNPs administration on Ehrlich solid tumor growth in mice.Ehrlich tumor bearing mice untreated with MNPs (♦), Ehrlich tumor bearing mice treated IP with MNPs (▪) and Ehrlich tumor bearing mice treated IT with MNPs (▴). Each point represents the mean±SE. n = 6. Representative images of tumor were shown for each group. Images were at the same magnification level.
Mentions: To assess the anti-tumor effect of MNPs; MNPs were injected IP and IT to Ehrlich tumor bearing mice, and tumor growth rate was measured for up to 4 weeks (Fig. 6). From day 14 to day 21, the tumor size of both MNPs treated groups was similar to that of untreated group without significant difference. After that, an observed significant reduction in tumor growth of IT treated mice (group 6) and less reduction in tumor size was observed in IP treated mice (group 5), while untreated mice showed continuous tumor growth (group 4). The growth inhibition of solid Ehrlich tumor was found to be 47.35% and 37.1% for IT and IP treated animals, respectively.

Bottom Line: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor.A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor.It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

ABSTRACT

Background: Magnetite nanoparticles (MNPs) have been widely used as contrast agents and have promising approaches in cancer treatment. In the present study we used Ehrlich solid carcinoma (ESC) bearing mice as a model to investigate MNPs antitumor activity, their effect on expression of p53 and p16 genes as an indicator for apoptotic induction in tumor tissues.

Method: MNPs coated with ascorbic acid (size: 25.0±5.0 nm) were synthesized by co-precipitation method and characterized. Ehrlich mice model were treated with MNPs using 60 mg/Kg day by day for 14 injections; intratumorally (IT) or intraperitoneally (IP). Tumor size, pathological changes and iron content in tumor and normal muscle tissues were assessed. We also assessed changes in expression levels of p53 and p16 genes in addition to p53 protein level by immunohistochemistry.

Results: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor. A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor. This increase was accompanied with increase in p53 protein expression. It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

Conclusion: MNPs might be more effective in breast cancer treatment if injected intratumorally to be directed to the tumor tissues.

Show MeSH
Related in: MedlinePlus