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Magnetite nanoparticles inhibit tumor growth and upregulate the expression of p53/p16 in Ehrlich solid carcinoma bearing mice.

Bassiony H, Sabet S, Salah El-Din TA, Mohamed MM, El-Ghor AA - PLoS ONE (2014)

Bottom Line: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor.A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor.It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

ABSTRACT

Background: Magnetite nanoparticles (MNPs) have been widely used as contrast agents and have promising approaches in cancer treatment. In the present study we used Ehrlich solid carcinoma (ESC) bearing mice as a model to investigate MNPs antitumor activity, their effect on expression of p53 and p16 genes as an indicator for apoptotic induction in tumor tissues.

Method: MNPs coated with ascorbic acid (size: 25.0±5.0 nm) were synthesized by co-precipitation method and characterized. Ehrlich mice model were treated with MNPs using 60 mg/Kg day by day for 14 injections; intratumorally (IT) or intraperitoneally (IP). Tumor size, pathological changes and iron content in tumor and normal muscle tissues were assessed. We also assessed changes in expression levels of p53 and p16 genes in addition to p53 protein level by immunohistochemistry.

Results: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor. A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor. This increase was accompanied with increase in p53 protein expression. It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

Conclusion: MNPs might be more effective in breast cancer treatment if injected intratumorally to be directed to the tumor tissues.

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Related in: MedlinePlus

HR-TEM image of the prepared MNPs capped with ascorbic acid shows that particles have spherical shape with average size of 20.0±2.0 nm.
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pone-0111960-g001: HR-TEM image of the prepared MNPs capped with ascorbic acid shows that particles have spherical shape with average size of 20.0±2.0 nm.

Mentions: Magnetite nanoparticles capped with L-ascorbic acid (Vitamin C) were synthesized. The HR-TEM image of the synthesized magnetite nanoparticles shows that these particles has average size of 20.0±2.0 nm with spherical shape as shown in Figure 1. VSM generated a hysteresis loop from which the saturation magnetization (Ms) was calculated under magnetic field lower than 10,000 Oersted at room temperature as shown in Figure 2. The saturation magnetization of the product is 5.2 emu/g. The small saturation magnetization in our case is most likely attributed to the much smaller size of Fe3O4 nanoparticles and the existence of surfactants (C6H6O6) on the surface. XRD phase analysis confirms the phase formation of MNPs as shown in Figure 3. Particle size analyzer shows that size of synthesized MNPs is 25.8 nm (Fig. 4).


Magnetite nanoparticles inhibit tumor growth and upregulate the expression of p53/p16 in Ehrlich solid carcinoma bearing mice.

Bassiony H, Sabet S, Salah El-Din TA, Mohamed MM, El-Ghor AA - PLoS ONE (2014)

HR-TEM image of the prepared MNPs capped with ascorbic acid shows that particles have spherical shape with average size of 20.0±2.0 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222961&req=5

pone-0111960-g001: HR-TEM image of the prepared MNPs capped with ascorbic acid shows that particles have spherical shape with average size of 20.0±2.0 nm.
Mentions: Magnetite nanoparticles capped with L-ascorbic acid (Vitamin C) were synthesized. The HR-TEM image of the synthesized magnetite nanoparticles shows that these particles has average size of 20.0±2.0 nm with spherical shape as shown in Figure 1. VSM generated a hysteresis loop from which the saturation magnetization (Ms) was calculated under magnetic field lower than 10,000 Oersted at room temperature as shown in Figure 2. The saturation magnetization of the product is 5.2 emu/g. The small saturation magnetization in our case is most likely attributed to the much smaller size of Fe3O4 nanoparticles and the existence of surfactants (C6H6O6) on the surface. XRD phase analysis confirms the phase formation of MNPs as shown in Figure 3. Particle size analyzer shows that size of synthesized MNPs is 25.8 nm (Fig. 4).

Bottom Line: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor.A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor.It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

ABSTRACT

Background: Magnetite nanoparticles (MNPs) have been widely used as contrast agents and have promising approaches in cancer treatment. In the present study we used Ehrlich solid carcinoma (ESC) bearing mice as a model to investigate MNPs antitumor activity, their effect on expression of p53 and p16 genes as an indicator for apoptotic induction in tumor tissues.

Method: MNPs coated with ascorbic acid (size: 25.0±5.0 nm) were synthesized by co-precipitation method and characterized. Ehrlich mice model were treated with MNPs using 60 mg/Kg day by day for 14 injections; intratumorally (IT) or intraperitoneally (IP). Tumor size, pathological changes and iron content in tumor and normal muscle tissues were assessed. We also assessed changes in expression levels of p53 and p16 genes in addition to p53 protein level by immunohistochemistry.

Results: Our results revealed that tumor growth was significantly reduced by IT and IP MNPs injection compared to untreated tumor. A significant increase in p53 and p16 mRNA expression was detected in Ehrlich solid tumors of IT and IP treated groups compared to untreated Ehrlich solid tumor. This increase was accompanied with increase in p53 protein expression. It is worth mentioning that no significant difference in expression of p53 and p16 could be detected between IT ESC and control group.

Conclusion: MNPs might be more effective in breast cancer treatment if injected intratumorally to be directed to the tumor tissues.

Show MeSH
Related in: MedlinePlus