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Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation.

Günal-Sadık G, Paszkowski-Rogacz M, Singaravelu K, Beyer A, Buchholz F, Jessberger R - PLoS ONE (2014)

Bottom Line: Specific binding changes of cohesin at B-cell specific gene loci Pax5 and Blimp-1 indicate new cohesin-dependent regulatory pathways.Together with conserved cohesin/CTCF sites at the Igh 3'RR, a prominent cohesin/CTCF binding site was revealed near the 3' end of Cα where PolII localizes to 3' enhancers.Our study shows that cohesin likely regulates B cell activation and maturation, including Ig class switching.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.

ABSTRACT
The immunoglobulin heavy chain locus (Igh) features higher-order chromosomal interactions to facilitate stage-specific assembly of the Ig molecule. Cohesin, a ring-like protein complex required for sister chromatid cohesion, shapes chromosome architecture and chromatin interactions important for transcriptional regulation and often acts together with CTCF. Cohesin is likely involved in B cell activation and Ig class switch recombination. Hence, binding profiles of cohesin in resting mature murine splenic B lymphocytes and at two stages after cell activation were elucidated by chromatin immunoprecipitation and deep sequencing. Comparative genomic analysis revealed cohesin extensively changes its binding to transcriptional control elements after 48 h of stimulation with LPS/IL-4. Cohesin was clearly underrepresented at switch regions regardless of their activation status, suggesting that switch regions need to be cohesin-poor. Specific binding changes of cohesin at B-cell specific gene loci Pax5 and Blimp-1 indicate new cohesin-dependent regulatory pathways. Together with conserved cohesin/CTCF sites at the Igh 3'RR, a prominent cohesin/CTCF binding site was revealed near the 3' end of Cα where PolII localizes to 3' enhancers. Our study shows that cohesin likely regulates B cell activation and maturation, including Ig class switching.

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Binding profiles at the Pax5 and Prdm1 gene loci.(A) Binding profiles at the Pax5 gene locus. ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Pax5 gene. (B) Binding profiles at the Prdm1 gene locus - ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Blimp-1 gene.
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pone-0111748-g004: Binding profiles at the Pax5 and Prdm1 gene loci.(A) Binding profiles at the Pax5 gene locus. ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Pax5 gene. (B) Binding profiles at the Prdm1 gene locus - ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Blimp-1 gene.

Mentions: The expression of B cell master regulator PAX5 is diminished as B cells differentiate into plasma cells upon completion of class switching [34]. Therefore, we investigated differential binding of cohesin at the Pax5 gene locus (Fig. 4A).


Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation.

Günal-Sadık G, Paszkowski-Rogacz M, Singaravelu K, Beyer A, Buchholz F, Jessberger R - PLoS ONE (2014)

Binding profiles at the Pax5 and Prdm1 gene loci.(A) Binding profiles at the Pax5 gene locus. ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Pax5 gene. (B) Binding profiles at the Prdm1 gene locus - ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Blimp-1 gene.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222939&req=5

pone-0111748-g004: Binding profiles at the Pax5 and Prdm1 gene loci.(A) Binding profiles at the Pax5 gene locus. ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Pax5 gene. (B) Binding profiles at the Prdm1 gene locus - ChIP-seq profiles of RAD21, CTCF, EBF1 and PolII binding at the Blimp-1 gene.
Mentions: The expression of B cell master regulator PAX5 is diminished as B cells differentiate into plasma cells upon completion of class switching [34]. Therefore, we investigated differential binding of cohesin at the Pax5 gene locus (Fig. 4A).

Bottom Line: Specific binding changes of cohesin at B-cell specific gene loci Pax5 and Blimp-1 indicate new cohesin-dependent regulatory pathways.Together with conserved cohesin/CTCF sites at the Igh 3'RR, a prominent cohesin/CTCF binding site was revealed near the 3' end of Cα where PolII localizes to 3' enhancers.Our study shows that cohesin likely regulates B cell activation and maturation, including Ig class switching.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.

ABSTRACT
The immunoglobulin heavy chain locus (Igh) features higher-order chromosomal interactions to facilitate stage-specific assembly of the Ig molecule. Cohesin, a ring-like protein complex required for sister chromatid cohesion, shapes chromosome architecture and chromatin interactions important for transcriptional regulation and often acts together with CTCF. Cohesin is likely involved in B cell activation and Ig class switch recombination. Hence, binding profiles of cohesin in resting mature murine splenic B lymphocytes and at two stages after cell activation were elucidated by chromatin immunoprecipitation and deep sequencing. Comparative genomic analysis revealed cohesin extensively changes its binding to transcriptional control elements after 48 h of stimulation with LPS/IL-4. Cohesin was clearly underrepresented at switch regions regardless of their activation status, suggesting that switch regions need to be cohesin-poor. Specific binding changes of cohesin at B-cell specific gene loci Pax5 and Blimp-1 indicate new cohesin-dependent regulatory pathways. Together with conserved cohesin/CTCF sites at the Igh 3'RR, a prominent cohesin/CTCF binding site was revealed near the 3' end of Cα where PolII localizes to 3' enhancers. Our study shows that cohesin likely regulates B cell activation and maturation, including Ig class switching.

Show MeSH