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Cell proliferation and neurogenesis in adult mouse brain.

Bordiuk OL, Smith K, Morin PJ, Semënov MV - PLoS ONE (2014)

Bottom Line: Despite significant progress in our understanding of adult neurogenesis, we are still missing data about the extent and location of production of neural precursors in the adult mammalian brain.The cable of proliferating cells emanating from the most anterior part of the CMS toward the olfactory bulbs forms the rostral migratory stream.The thin layer of proliferating cells extending posteriorly from the CMS forms the midlayer.

View Article: PubMed Central - PubMed

Affiliation: New England Geriatric Research Education and Clinical Center, Bedford Division, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, Massachusetts, United States of America.

ABSTRACT
Neurogenesis, the formation of new neurons, can be observed in the adult brain of many mammalian species, including humans. Despite significant progress in our understanding of adult neurogenesis, we are still missing data about the extent and location of production of neural precursors in the adult mammalian brain. We used 5-ethynyl-2'-deoxyuridine (EdU) to map the location of proliferating cells throughout the entire adult mouse brain and found that neurogenesis occurs at two locations in the mouse brain. The larger one we define as the main proliferative zone (MPZ), and the smaller one corresponds to the subgranular zone of the hippocampus. The MPZ can be divided into three parts. The caudate migratory stream (CMS) occupies the middle part of the MPZ. The cable of proliferating cells emanating from the most anterior part of the CMS toward the olfactory bulbs forms the rostral migratory stream. The thin layer of proliferating cells extending posteriorly from the CMS forms the midlayer. We have not found any additional aggregations of proliferating cells in the adult mouse brain that could suggest the existence of other major neurogenic zones in the adult mouse brain.

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The Main Proliferative Zone (MPZ) appears as an uninterrupted continuum in which EdU-labeled nuclei from each structure occupy well-defined locations.A. Each EdU-labeled nucleus in the MPZ is shown as a black dot. B. Each EdU-labeled nucleus in the BetwH is shown as a magenta dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. C. Each EdU-labeled nucleus in the Caud is shown as a blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. D. Each EdU-labeled nucleus in the DorW is shown as a pink dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. E. Each EdU-labeled nucleus in the FusW is shown as an orange dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. F. Each EdU-labeled nucleus in the LatW is shown as a yellow dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. G. Each EdU-labeled nucleus in the MedW is shown as a light blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. H. Each EdU-labeled nucleus in the OB is shown as an olive dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. I. Each EdU-labeled nucleus in the PostH is shown as a cyan dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. J. Each EdU-labeled nucleus in the RMS is shown as a lilac dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. K. Each EdU-labeled nucleus in the SupAl is shown as a red dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. L. Each EdU-labeled nucleus in the MPZ is shown as a color dot with colors assigned according to the Table 2. Abbreviated names are given according to Table 2. The brain shape is outlined with a black line. Lateral view. 120 days-old mouse one hour post EdU injection.
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pone-0111453-g005: The Main Proliferative Zone (MPZ) appears as an uninterrupted continuum in which EdU-labeled nuclei from each structure occupy well-defined locations.A. Each EdU-labeled nucleus in the MPZ is shown as a black dot. B. Each EdU-labeled nucleus in the BetwH is shown as a magenta dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. C. Each EdU-labeled nucleus in the Caud is shown as a blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. D. Each EdU-labeled nucleus in the DorW is shown as a pink dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. E. Each EdU-labeled nucleus in the FusW is shown as an orange dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. F. Each EdU-labeled nucleus in the LatW is shown as a yellow dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. G. Each EdU-labeled nucleus in the MedW is shown as a light blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. H. Each EdU-labeled nucleus in the OB is shown as an olive dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. I. Each EdU-labeled nucleus in the PostH is shown as a cyan dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. J. Each EdU-labeled nucleus in the RMS is shown as a lilac dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. K. Each EdU-labeled nucleus in the SupAl is shown as a red dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. L. Each EdU-labeled nucleus in the MPZ is shown as a color dot with colors assigned according to the Table 2. Abbreviated names are given according to Table 2. The brain shape is outlined with a black line. Lateral view. 120 days-old mouse one hour post EdU injection.

Mentions: We examined brain sections and selected eight additional brain structures that appeared to be enriched with an abundance of proliferating cells (Table 2, Figure 3A–D). Next, we obtained coordinates of EdU-labeled nuclei in each of these structures and visualized their distribution inside the mouse brain as a point cloud with different colors assigned to cells from each structure (Table 2, Figure 4A). The point cloud of all selected cells (Figure 4B) closely resembled the aggregation (Figure 2B). Unselected EdU-labeled nuclei showed no additional aggregations (Figure 4C). EdU-labeled nuclei in the aggregation formed two separate point clouds. The cells distributed along the DG formed one cloud (Figure 4D), and the cells in the ten other structures formed another that we named the Main Proliferative Zone (MPZ). The MPZ appears as an uninterrupted continuum (Figure 4E) in which EdU-labeled nuclei from each structure occupy well-defined locations (Figure 5). We also found that cells expressing doublecortin (DCX) are present in all structures comprising the MPZ (Figure 6).


Cell proliferation and neurogenesis in adult mouse brain.

Bordiuk OL, Smith K, Morin PJ, Semënov MV - PLoS ONE (2014)

The Main Proliferative Zone (MPZ) appears as an uninterrupted continuum in which EdU-labeled nuclei from each structure occupy well-defined locations.A. Each EdU-labeled nucleus in the MPZ is shown as a black dot. B. Each EdU-labeled nucleus in the BetwH is shown as a magenta dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. C. Each EdU-labeled nucleus in the Caud is shown as a blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. D. Each EdU-labeled nucleus in the DorW is shown as a pink dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. E. Each EdU-labeled nucleus in the FusW is shown as an orange dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. F. Each EdU-labeled nucleus in the LatW is shown as a yellow dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. G. Each EdU-labeled nucleus in the MedW is shown as a light blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. H. Each EdU-labeled nucleus in the OB is shown as an olive dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. I. Each EdU-labeled nucleus in the PostH is shown as a cyan dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. J. Each EdU-labeled nucleus in the RMS is shown as a lilac dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. K. Each EdU-labeled nucleus in the SupAl is shown as a red dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. L. Each EdU-labeled nucleus in the MPZ is shown as a color dot with colors assigned according to the Table 2. Abbreviated names are given according to Table 2. The brain shape is outlined with a black line. Lateral view. 120 days-old mouse one hour post EdU injection.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4222938&req=5

pone-0111453-g005: The Main Proliferative Zone (MPZ) appears as an uninterrupted continuum in which EdU-labeled nuclei from each structure occupy well-defined locations.A. Each EdU-labeled nucleus in the MPZ is shown as a black dot. B. Each EdU-labeled nucleus in the BetwH is shown as a magenta dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. C. Each EdU-labeled nucleus in the Caud is shown as a blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. D. Each EdU-labeled nucleus in the DorW is shown as a pink dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. E. Each EdU-labeled nucleus in the FusW is shown as an orange dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. F. Each EdU-labeled nucleus in the LatW is shown as a yellow dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. G. Each EdU-labeled nucleus in the MedW is shown as a light blue dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. H. Each EdU-labeled nucleus in the OB is shown as an olive dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. I. Each EdU-labeled nucleus in the PostH is shown as a cyan dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. J. Each EdU-labeled nucleus in the RMS is shown as a lilac dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. K. Each EdU-labeled nucleus in the SupAl is shown as a red dot. EdU-labeled nuclei in other parts of the MPZ are shown as black dots. L. Each EdU-labeled nucleus in the MPZ is shown as a color dot with colors assigned according to the Table 2. Abbreviated names are given according to Table 2. The brain shape is outlined with a black line. Lateral view. 120 days-old mouse one hour post EdU injection.
Mentions: We examined brain sections and selected eight additional brain structures that appeared to be enriched with an abundance of proliferating cells (Table 2, Figure 3A–D). Next, we obtained coordinates of EdU-labeled nuclei in each of these structures and visualized their distribution inside the mouse brain as a point cloud with different colors assigned to cells from each structure (Table 2, Figure 4A). The point cloud of all selected cells (Figure 4B) closely resembled the aggregation (Figure 2B). Unselected EdU-labeled nuclei showed no additional aggregations (Figure 4C). EdU-labeled nuclei in the aggregation formed two separate point clouds. The cells distributed along the DG formed one cloud (Figure 4D), and the cells in the ten other structures formed another that we named the Main Proliferative Zone (MPZ). The MPZ appears as an uninterrupted continuum (Figure 4E) in which EdU-labeled nuclei from each structure occupy well-defined locations (Figure 5). We also found that cells expressing doublecortin (DCX) are present in all structures comprising the MPZ (Figure 6).

Bottom Line: Despite significant progress in our understanding of adult neurogenesis, we are still missing data about the extent and location of production of neural precursors in the adult mammalian brain.The cable of proliferating cells emanating from the most anterior part of the CMS toward the olfactory bulbs forms the rostral migratory stream.The thin layer of proliferating cells extending posteriorly from the CMS forms the midlayer.

View Article: PubMed Central - PubMed

Affiliation: New England Geriatric Research Education and Clinical Center, Bedford Division, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, Massachusetts, United States of America.

ABSTRACT
Neurogenesis, the formation of new neurons, can be observed in the adult brain of many mammalian species, including humans. Despite significant progress in our understanding of adult neurogenesis, we are still missing data about the extent and location of production of neural precursors in the adult mammalian brain. We used 5-ethynyl-2'-deoxyuridine (EdU) to map the location of proliferating cells throughout the entire adult mouse brain and found that neurogenesis occurs at two locations in the mouse brain. The larger one we define as the main proliferative zone (MPZ), and the smaller one corresponds to the subgranular zone of the hippocampus. The MPZ can be divided into three parts. The caudate migratory stream (CMS) occupies the middle part of the MPZ. The cable of proliferating cells emanating from the most anterior part of the CMS toward the olfactory bulbs forms the rostral migratory stream. The thin layer of proliferating cells extending posteriorly from the CMS forms the midlayer. We have not found any additional aggregations of proliferating cells in the adult mouse brain that could suggest the existence of other major neurogenic zones in the adult mouse brain.

Show MeSH
Related in: MedlinePlus