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Xenogeneic acellular conjunctiva matrix as a scaffold of tissue-engineered corneal epithelium.

Zhao H, Qu M, Wang Y, Wang Z, Shi W - PLoS ONE (2014)

Bottom Line: In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium.The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM.These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, Shandong, China.

ABSTRACT
Amniotic membrane-based tissue-engineered corneal epithelium has been widely used in the reconstruction of the ocular surface. However, it often degrades too early to ensure the success of the transplanted corneal epithelium when treating patients with severe ocular surface disorders. In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium. Native porcine conjunctiva was decellularized with 0.1% sodium dodecyl sulfate (SDS) for 12 h at 37°C and sterilized via γ-irradiation. Compared with native conjunctiva, more than 92% of the DNA was removed, and more than 90% of the extracellular matrix components (glycosaminoglycan and collagen) remained after the decellularization treatment. Compared with denuded amniotic membrane (dAM), the aCM possessed favorable optical transmittance, tensile strength, stability and biocompatibility as well as stronger resistance to degradation both in vitro and in vivo. The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM. The aCM-based tissue-engineered corneal epithelium was more effective in the reconstruction of the ocular surface in rabbits with limbal stem cell deficiency. These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

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Related in: MedlinePlus

Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).
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pone-0111846-g009: Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).

Mentions: Restoration of the corneal epithelium began at day 7 among rabbits that had been transplanted with aCM-based reconstructed corneal epithelium. Restoration was complete at day 30, with the appearance of normal corneal epithelium and no visible staining of goblet cells. In rabbit corneas transplanted with dAM-based reconstructed corneal epithelium, repair had not begun at day 7, and goblet cells could be observed until day 30 postoperatively (Fig. 9). Furthermore, more donor cells could be detected on the peripheral cornea transplanted with aCM-based reconstructed corneal epithelium compared with those transplanted with dAM-based epithelium at days 7 and 30. On the central cornea, there were more donor cells in the aCM group at day 30, although there were no differences between the groups at day 7 (Fig. 10).


Xenogeneic acellular conjunctiva matrix as a scaffold of tissue-engineered corneal epithelium.

Zhao H, Qu M, Wang Y, Wang Z, Shi W - PLoS ONE (2014)

Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222936&req=5

pone-0111846-g009: Corneal H and E staining and impression cytology after transplantation of tissue-engineered corneal epithelium.Restoration of corneal epithelium began at day 7 after transplantation with reconstructed corneal epithelium on aCM (A). Normal morphology was observed at day 30 (B) with no goblet cells (C), whereas the group that received reconstructed corneal epithelium on dAM exhibited defective corneal epithelium at day 7 (D) and goblet cells (arrow) were still observed at day 30 (E, F). Scale bar: 100 µm (A, B, D, E) and 10 µm (C, F).
Mentions: Restoration of the corneal epithelium began at day 7 among rabbits that had been transplanted with aCM-based reconstructed corneal epithelium. Restoration was complete at day 30, with the appearance of normal corneal epithelium and no visible staining of goblet cells. In rabbit corneas transplanted with dAM-based reconstructed corneal epithelium, repair had not begun at day 7, and goblet cells could be observed until day 30 postoperatively (Fig. 9). Furthermore, more donor cells could be detected on the peripheral cornea transplanted with aCM-based reconstructed corneal epithelium compared with those transplanted with dAM-based epithelium at days 7 and 30. On the central cornea, there were more donor cells in the aCM group at day 30, although there were no differences between the groups at day 7 (Fig. 10).

Bottom Line: In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium.The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM.These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, Shandong, China.

ABSTRACT
Amniotic membrane-based tissue-engineered corneal epithelium has been widely used in the reconstruction of the ocular surface. However, it often degrades too early to ensure the success of the transplanted corneal epithelium when treating patients with severe ocular surface disorders. In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium. Native porcine conjunctiva was decellularized with 0.1% sodium dodecyl sulfate (SDS) for 12 h at 37°C and sterilized via γ-irradiation. Compared with native conjunctiva, more than 92% of the DNA was removed, and more than 90% of the extracellular matrix components (glycosaminoglycan and collagen) remained after the decellularization treatment. Compared with denuded amniotic membrane (dAM), the aCM possessed favorable optical transmittance, tensile strength, stability and biocompatibility as well as stronger resistance to degradation both in vitro and in vivo. The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM. The aCM-based tissue-engineered corneal epithelium was more effective in the reconstruction of the ocular surface in rabbits with limbal stem cell deficiency. These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

Show MeSH
Related in: MedlinePlus