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Xenogeneic acellular conjunctiva matrix as a scaffold of tissue-engineered corneal epithelium.

Zhao H, Qu M, Wang Y, Wang Z, Shi W - PLoS ONE (2014)

Bottom Line: In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium.The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM.These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, Shandong, China.

ABSTRACT
Amniotic membrane-based tissue-engineered corneal epithelium has been widely used in the reconstruction of the ocular surface. However, it often degrades too early to ensure the success of the transplanted corneal epithelium when treating patients with severe ocular surface disorders. In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium. Native porcine conjunctiva was decellularized with 0.1% sodium dodecyl sulfate (SDS) for 12 h at 37°C and sterilized via γ-irradiation. Compared with native conjunctiva, more than 92% of the DNA was removed, and more than 90% of the extracellular matrix components (glycosaminoglycan and collagen) remained after the decellularization treatment. Compared with denuded amniotic membrane (dAM), the aCM possessed favorable optical transmittance, tensile strength, stability and biocompatibility as well as stronger resistance to degradation both in vitro and in vivo. The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM. The aCM-based tissue-engineered corneal epithelium was more effective in the reconstruction of the ocular surface in rabbits with limbal stem cell deficiency. These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

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Construction of a rabbit limbal stem cell deficiency model.Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).
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pone-0111846-g007: Construction of a rabbit limbal stem cell deficiency model.Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).

Mentions: Corneal opacity and neovascularization could easily be observed one month postoperatively (Fig. 7A). Eighteen of the 20 rabbits scored ≥2 in corneal opacity and ≥3 in corneal neovascularization; imprint cytology revealed goblet cells with H.E. staining and positive periodic acid-shiff's (PAS) staining in corneas from two randomly selected rabbits (Fig. 7B, C).


Xenogeneic acellular conjunctiva matrix as a scaffold of tissue-engineered corneal epithelium.

Zhao H, Qu M, Wang Y, Wang Z, Shi W - PLoS ONE (2014)

Construction of a rabbit limbal stem cell deficiency model.Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222936&req=5

pone-0111846-g007: Construction of a rabbit limbal stem cell deficiency model.Representative slit-lamp photograph of rabbit cornea showed significant opacity and neovascularization after 1 month (A). PAS staining in which goblet cells are easily observed in corneal impression cytology (B). Signet ring goblet cells (arrow) were also revealed by H.E. staining of corneal sections (C). Scale bar: 10 µm (B) and 50 µm (C).
Mentions: Corneal opacity and neovascularization could easily be observed one month postoperatively (Fig. 7A). Eighteen of the 20 rabbits scored ≥2 in corneal opacity and ≥3 in corneal neovascularization; imprint cytology revealed goblet cells with H.E. staining and positive periodic acid-shiff's (PAS) staining in corneas from two randomly selected rabbits (Fig. 7B, C).

Bottom Line: In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium.The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM.These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, Shandong, China.

ABSTRACT
Amniotic membrane-based tissue-engineered corneal epithelium has been widely used in the reconstruction of the ocular surface. However, it often degrades too early to ensure the success of the transplanted corneal epithelium when treating patients with severe ocular surface disorders. In the present study, we investigated the preparation of xenogeneic acellular conjunctiva matrix (aCM) and evaluated its efficacy and safety as a scaffold of tissue-engineered corneal epithelium. Native porcine conjunctiva was decellularized with 0.1% sodium dodecyl sulfate (SDS) for 12 h at 37°C and sterilized via γ-irradiation. Compared with native conjunctiva, more than 92% of the DNA was removed, and more than 90% of the extracellular matrix components (glycosaminoglycan and collagen) remained after the decellularization treatment. Compared with denuded amniotic membrane (dAM), the aCM possessed favorable optical transmittance, tensile strength, stability and biocompatibility as well as stronger resistance to degradation both in vitro and in vivo. The corneal epithelial cells seeded on aCM formed a multilayered epithelial structure and endured longer than did those on dAM. The aCM-based tissue-engineered corneal epithelium was more effective in the reconstruction of the ocular surface in rabbits with limbal stem cell deficiency. These findings support the application of xenogeneic acellular conjunctiva matrix as a scaffold for reconstructing the ocular surface.

Show MeSH
Related in: MedlinePlus