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Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery.

Clauser L, Ferroni L, Gardin C, Tieghi R, Galiè M, Elia G, Piattelli A, Pinton P, Bressan E, Zavan B - PLoS ONE (2014)

Bottom Line: The obtained fat volumes were divided into three layers and then analyzed.The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers.The low-density layer exhibits an enrichment of multipotent stem cells.

View Article: PubMed Central - PubMed

Affiliation: Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.

ABSTRACT
Structural fat grafting utilizes the centrifugation of liposuction aspirates to create a graded density of adipose tissue. This study was performed to qualitatively investigate the effects of centrifugation on stem cells present in adipose tissue. Liposuction aspirates were obtained from healthy donors and either not centrifuged or centrifuged at 1,800 rpm for 3 minutes. The obtained fat volumes were divided into three layers and then analyzed. The results demonstrate that centrifugation induces a different distribution of stem cells in the three layers. The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers. The low-density layer exhibits an enrichment of multipotent stem cells. We conclude that appropriate centrifugation concentrates stem cells. This finding may influence the clinical practice of liposuction aspirate centrifugation and enhance graft uptake.

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Gene expression profile of stemness markers.Gene expression profile of stemness markers in the LDL (white bars), MDL (grey bars), and HDL (black bars). The results are reported as ratios (R) with respect to the mRNA expression levels of non-centrifuged fat (not shown).
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pone-0110796-g002: Gene expression profile of stemness markers.Gene expression profile of stemness markers in the LDL (white bars), MDL (grey bars), and HDL (black bars). The results are reported as ratios (R) with respect to the mRNA expression levels of non-centrifuged fat (not shown).

Mentions: To characterize the stemness properties of the cells within the fat, the mRNA from each layer was extracted and analyzed by real-time PCR. The analyzed genes are listed in Table 1. Figure 2 shows the gene expression of stemness markers in the LDL, MDL, and HDL. Non-centrifuged fat was used as a control. Significant mRNA expression was detected for LIF, SOX2, TERT, WNT3A, and ZFP42. The HDL exhibited higher levels of LIF and SOX2 gene expression compared with those expression levels in the MDL and in the LDL, whereas the LDL displayed higher expression of the ZFP42 gene, which is a marker of pluripotent stem cells [28].


Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery.

Clauser L, Ferroni L, Gardin C, Tieghi R, Galiè M, Elia G, Piattelli A, Pinton P, Bressan E, Zavan B - PLoS ONE (2014)

Gene expression profile of stemness markers.Gene expression profile of stemness markers in the LDL (white bars), MDL (grey bars), and HDL (black bars). The results are reported as ratios (R) with respect to the mRNA expression levels of non-centrifuged fat (not shown).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222876&req=5

pone-0110796-g002: Gene expression profile of stemness markers.Gene expression profile of stemness markers in the LDL (white bars), MDL (grey bars), and HDL (black bars). The results are reported as ratios (R) with respect to the mRNA expression levels of non-centrifuged fat (not shown).
Mentions: To characterize the stemness properties of the cells within the fat, the mRNA from each layer was extracted and analyzed by real-time PCR. The analyzed genes are listed in Table 1. Figure 2 shows the gene expression of stemness markers in the LDL, MDL, and HDL. Non-centrifuged fat was used as a control. Significant mRNA expression was detected for LIF, SOX2, TERT, WNT3A, and ZFP42. The HDL exhibited higher levels of LIF and SOX2 gene expression compared with those expression levels in the MDL and in the LDL, whereas the LDL displayed higher expression of the ZFP42 gene, which is a marker of pluripotent stem cells [28].

Bottom Line: The obtained fat volumes were divided into three layers and then analyzed.The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers.The low-density layer exhibits an enrichment of multipotent stem cells.

View Article: PubMed Central - PubMed

Affiliation: Unit of Cranio Maxillo Facial Surgery, Center for Craniofacial Deformities and Orbital Surgery - Reference Center for Rare Disease, Sant'Anna Hospital and University, Cona, Ferrara, Italy.

ABSTRACT
Structural fat grafting utilizes the centrifugation of liposuction aspirates to create a graded density of adipose tissue. This study was performed to qualitatively investigate the effects of centrifugation on stem cells present in adipose tissue. Liposuction aspirates were obtained from healthy donors and either not centrifuged or centrifuged at 1,800 rpm for 3 minutes. The obtained fat volumes were divided into three layers and then analyzed. The results demonstrate that centrifugation induces a different distribution of stem cells in the three layers. The high-density layer displays the highest expression of mesenchymal stem cell and endothelial markers. The low-density layer exhibits an enrichment of multipotent stem cells. We conclude that appropriate centrifugation concentrates stem cells. This finding may influence the clinical practice of liposuction aspirate centrifugation and enhance graft uptake.

Show MeSH
Related in: MedlinePlus