Limits...
RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients.

Huang X, Hao C, Shen X, Zhang Y, Liu X - Reprod. Biol. Endocrinol. (2013)

Bottom Line: Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied.The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization.GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence.

View Article: PubMed Central - HTML - PubMed

Affiliation: Reproductive Medicine Centre, Affiliated Hospital of Qingdao Medical University, Yuhuangding Hospital of Yantai, 20 Yuhuangding Road East, Yantai, Shandong, China. cuifanghao@aliyun.com.

ABSTRACT

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The developmental competence of oocytes and embryos in PCOS patients is reduced to a certain extent (comparing to non-PCOS patients, the high quality embryo rate was decreased by 16% from the data of our centre) during the in vitro fertilization (IVF) process. Cross-talk between the oocyte and cumulus cells is critical for oocyte maturation and embryo competence. In this study, we have evaluated the transcription of specific genes in cumulus cells harvested from pre-ovulatory follicles of PCOS patients before IVF, according to individual oocyte nuclear maturity and developmental competence. Seven genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, and ITGB5) were targeted from our previous cDNA microarray data which isolated genes related to oocyte nuclear maturation in PCOS patients. Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied.

Methods: The mRNA expression levels of cumulus cells were detected by qRT- PCR.

Results: Consistent with our previous cDNA microarray data, with the exception of GPX3 and PTX3, the selected 7 genes were related to oocyte nuclear maturation in PCOS patients. Noticeably, the expression level of RUNX2 was lower in cumulus cells derived from oocytes that could develop into blastocysts than the level of expression from oocytes that could not. The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization. GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence.

Conclusions: Several cumulus cell genes were associated with oocyte maturation, fertilization and embryo quality in PCOS patients. RUNX2 and GPX3 are candidate genetic markers in the monitoring of embryo quality for PCOS patients, whereas PTX3 mainly played a role in fertilization process. Together with morphological evaluation, cumulus cells genes may serve as biomarkers of oocyte and embryo selection during the IVF process for PCOS patients and may advance our understanding of PCOS.

Show MeSH

Related in: MedlinePlus

Transcripts levels of the target genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) according to oocyte nuclear maturity in PCOS patients. mRNA expression of candidate genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) in cumulus cells of PCOS patients, organized according to oocyte nuclear maturity stage (MI/GV stage versus MII stage). The signal intensity for each gene is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogeneous reference. * indicates a significant difference in gene expression between CC categories (** p < 0.01, * p < 0.05). The results are presented as the means ± SEM. CCMI/GV: cumulus cells from oocyte at the MI stage or GV stage; CCMII: cumulus cells from oocyte at the MII stage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4222840&req=5

Figure 2: Transcripts levels of the target genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) according to oocyte nuclear maturity in PCOS patients. mRNA expression of candidate genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) in cumulus cells of PCOS patients, organized according to oocyte nuclear maturity stage (MI/GV stage versus MII stage). The signal intensity for each gene is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogeneous reference. * indicates a significant difference in gene expression between CC categories (** p < 0.01, * p < 0.05). The results are presented as the means ± SEM. CCMI/GV: cumulus cells from oocyte at the MI stage or GV stage; CCMII: cumulus cells from oocyte at the MII stage.

Mentions: In the CCMII (n = 173) compared to CCMI/GV (n = 33) groups (Figure 2), mean transcript levels of three genes were significantly higher, with 2.18-, 3.37- and 2.26- fold increases observed for RUNX2 (2.14 ± 0.63 versus 0.98 ± 0.33), PSAT1 (4.38 ± 0.92 versus 1.3 ± 0.3), and ADAMTS9 (1.65 ± 0.3 versus 0.73 ± 0.23), respectively, whereas the expression levels of four genes were significantly decreased in the CCMII samples, with 4.05-, 4.21-, 6- and 3.27- fold decreases observed for CXCL1 (0.19 ± 0.09 versus 0.77 ± 0.26), CXCL2 (0.19 ± 0.02 versus 0.8 ± 0.18), CXCL3 (0.16 ± 0.02 versus 0.96 ± 0.09) and ITGB5 (0.26 ± 0.03 versus 0.85 ± 0.13), respectively. The transcripts levels of the other two genes (GPX3 and PTX3) exhibited no significant differences between the CCMII and CCMI/GV groups.


RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients.

Huang X, Hao C, Shen X, Zhang Y, Liu X - Reprod. Biol. Endocrinol. (2013)

Transcripts levels of the target genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) according to oocyte nuclear maturity in PCOS patients. mRNA expression of candidate genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) in cumulus cells of PCOS patients, organized according to oocyte nuclear maturity stage (MI/GV stage versus MII stage). The signal intensity for each gene is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogeneous reference. * indicates a significant difference in gene expression between CC categories (** p < 0.01, * p < 0.05). The results are presented as the means ± SEM. CCMI/GV: cumulus cells from oocyte at the MI stage or GV stage; CCMII: cumulus cells from oocyte at the MII stage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222840&req=5

Figure 2: Transcripts levels of the target genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) according to oocyte nuclear maturity in PCOS patients. mRNA expression of candidate genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) in cumulus cells of PCOS patients, organized according to oocyte nuclear maturity stage (MI/GV stage versus MII stage). The signal intensity for each gene is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogeneous reference. * indicates a significant difference in gene expression between CC categories (** p < 0.01, * p < 0.05). The results are presented as the means ± SEM. CCMI/GV: cumulus cells from oocyte at the MI stage or GV stage; CCMII: cumulus cells from oocyte at the MII stage.
Mentions: In the CCMII (n = 173) compared to CCMI/GV (n = 33) groups (Figure 2), mean transcript levels of three genes were significantly higher, with 2.18-, 3.37- and 2.26- fold increases observed for RUNX2 (2.14 ± 0.63 versus 0.98 ± 0.33), PSAT1 (4.38 ± 0.92 versus 1.3 ± 0.3), and ADAMTS9 (1.65 ± 0.3 versus 0.73 ± 0.23), respectively, whereas the expression levels of four genes were significantly decreased in the CCMII samples, with 4.05-, 4.21-, 6- and 3.27- fold decreases observed for CXCL1 (0.19 ± 0.09 versus 0.77 ± 0.26), CXCL2 (0.19 ± 0.02 versus 0.8 ± 0.18), CXCL3 (0.16 ± 0.02 versus 0.96 ± 0.09) and ITGB5 (0.26 ± 0.03 versus 0.85 ± 0.13), respectively. The transcripts levels of the other two genes (GPX3 and PTX3) exhibited no significant differences between the CCMII and CCMI/GV groups.

Bottom Line: Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied.The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization.GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence.

View Article: PubMed Central - HTML - PubMed

Affiliation: Reproductive Medicine Centre, Affiliated Hospital of Qingdao Medical University, Yuhuangding Hospital of Yantai, 20 Yuhuangding Road East, Yantai, Shandong, China. cuifanghao@aliyun.com.

ABSTRACT

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The developmental competence of oocytes and embryos in PCOS patients is reduced to a certain extent (comparing to non-PCOS patients, the high quality embryo rate was decreased by 16% from the data of our centre) during the in vitro fertilization (IVF) process. Cross-talk between the oocyte and cumulus cells is critical for oocyte maturation and embryo competence. In this study, we have evaluated the transcription of specific genes in cumulus cells harvested from pre-ovulatory follicles of PCOS patients before IVF, according to individual oocyte nuclear maturity and developmental competence. Seven genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, and ITGB5) were targeted from our previous cDNA microarray data which isolated genes related to oocyte nuclear maturation in PCOS patients. Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied.

Methods: The mRNA expression levels of cumulus cells were detected by qRT- PCR.

Results: Consistent with our previous cDNA microarray data, with the exception of GPX3 and PTX3, the selected 7 genes were related to oocyte nuclear maturation in PCOS patients. Noticeably, the expression level of RUNX2 was lower in cumulus cells derived from oocytes that could develop into blastocysts than the level of expression from oocytes that could not. The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization. GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence.

Conclusions: Several cumulus cell genes were associated with oocyte maturation, fertilization and embryo quality in PCOS patients. RUNX2 and GPX3 are candidate genetic markers in the monitoring of embryo quality for PCOS patients, whereas PTX3 mainly played a role in fertilization process. Together with morphological evaluation, cumulus cells genes may serve as biomarkers of oocyte and embryo selection during the IVF process for PCOS patients and may advance our understanding of PCOS.

Show MeSH
Related in: MedlinePlus