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Glial enriched gene expression profiling identifies novel factors regulating the proliferation of specific glial subtypes in the Drosophila brain.

Avet-Rochex A, Maierbrugger KT, Bateman JM - Gene Expr. Patterns (2014)

Bottom Line: Analysis of the differentially regulated genes in these tissues shows that the expression of known glial genes is significantly increased in both cases.Conversely, the expression of neuronal genes is significantly decreased.We then used these data to identify novel transcription factors that are expressed in glia in the brain.

View Article: PubMed Central - PubMed

Affiliation: Wolfson Centre for Age-Related Diseases, King's College London, Guy's Campus, London SE1 1UL, UK.

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Related in: MedlinePlus

 Generation of larval CNS tissue enriched in glia. (A) Late third instar larval CNS expressing nuclear GFP in glia using repo-Gal4 (repo>nGFP). (B,C) Overexpression of HtlACT (B), or the InR (C) in glia using repo-Gal4 causes glial overproliferation. Glia are marked by the expression of nuclear GFP as in A. (D–F') Overexpression of HtlACT (E), but not the InR (F), in glia using repo-Gal4 causes overproliferation of PntP2 expressing cortex glia. PntP2 expression shown in magenta (D–F') and glia (green in D–E') are marked by the expression of nuclear GFP as in (A–C).
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f0010:  Generation of larval CNS tissue enriched in glia. (A) Late third instar larval CNS expressing nuclear GFP in glia using repo-Gal4 (repo>nGFP). (B,C) Overexpression of HtlACT (B), or the InR (C) in glia using repo-Gal4 causes glial overproliferation. Glia are marked by the expression of nuclear GFP as in A. (D–F') Overexpression of HtlACT (E), but not the InR (F), in glia using repo-Gal4 causes overproliferation of PntP2 expressing cortex glia. PntP2 expression shown in magenta (D–F') and glia (green in D–E') are marked by the expression of nuclear GFP as in (A–C).

Mentions: We have recently shown that the proliferation of two glial subtypes in the Drosophila post-embryonic brain is regulated through the concerted action of the FGF and InR/mTOR pathways. Cortex glia require FGF signalling and the InR, but not downstream components of the InR/mTOR pathway, whereas perineurial glia require both FGF and InR signalling pathways for proliferation. Pan-glial activation of either pathway causes glial overproliferation (Fig. 1B,C). However, specific glial sub-types respond differently to the expression of each receptor. The majority of superficial glia in larval brains from animals overexpressing an activated form of the FGF receptor (HtlACT) in glia expressed both the pan-glial protein Repo and pointedP2 (PntP2), a marker of cortex glia (Fig. 1E,E'). By contrast, glial-specific overexpression of the InR resulted in the proliferation of Repo expressing, but not PntP2 expressing glia (Fig. 1F,F'). These data suggest that these two receptors promote glial proliferation, but that the glial subtypes that proliferate are partially distinct.


Glial enriched gene expression profiling identifies novel factors regulating the proliferation of specific glial subtypes in the Drosophila brain.

Avet-Rochex A, Maierbrugger KT, Bateman JM - Gene Expr. Patterns (2014)

 Generation of larval CNS tissue enriched in glia. (A) Late third instar larval CNS expressing nuclear GFP in glia using repo-Gal4 (repo>nGFP). (B,C) Overexpression of HtlACT (B), or the InR (C) in glia using repo-Gal4 causes glial overproliferation. Glia are marked by the expression of nuclear GFP as in A. (D–F') Overexpression of HtlACT (E), but not the InR (F), in glia using repo-Gal4 causes overproliferation of PntP2 expressing cortex glia. PntP2 expression shown in magenta (D–F') and glia (green in D–E') are marked by the expression of nuclear GFP as in (A–C).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4222725&req=5

f0010:  Generation of larval CNS tissue enriched in glia. (A) Late third instar larval CNS expressing nuclear GFP in glia using repo-Gal4 (repo>nGFP). (B,C) Overexpression of HtlACT (B), or the InR (C) in glia using repo-Gal4 causes glial overproliferation. Glia are marked by the expression of nuclear GFP as in A. (D–F') Overexpression of HtlACT (E), but not the InR (F), in glia using repo-Gal4 causes overproliferation of PntP2 expressing cortex glia. PntP2 expression shown in magenta (D–F') and glia (green in D–E') are marked by the expression of nuclear GFP as in (A–C).
Mentions: We have recently shown that the proliferation of two glial subtypes in the Drosophila post-embryonic brain is regulated through the concerted action of the FGF and InR/mTOR pathways. Cortex glia require FGF signalling and the InR, but not downstream components of the InR/mTOR pathway, whereas perineurial glia require both FGF and InR signalling pathways for proliferation. Pan-glial activation of either pathway causes glial overproliferation (Fig. 1B,C). However, specific glial sub-types respond differently to the expression of each receptor. The majority of superficial glia in larval brains from animals overexpressing an activated form of the FGF receptor (HtlACT) in glia expressed both the pan-glial protein Repo and pointedP2 (PntP2), a marker of cortex glia (Fig. 1E,E'). By contrast, glial-specific overexpression of the InR resulted in the proliferation of Repo expressing, but not PntP2 expressing glia (Fig. 1F,F'). These data suggest that these two receptors promote glial proliferation, but that the glial subtypes that proliferate are partially distinct.

Bottom Line: Analysis of the differentially regulated genes in these tissues shows that the expression of known glial genes is significantly increased in both cases.Conversely, the expression of neuronal genes is significantly decreased.We then used these data to identify novel transcription factors that are expressed in glia in the brain.

View Article: PubMed Central - PubMed

Affiliation: Wolfson Centre for Age-Related Diseases, King's College London, Guy's Campus, London SE1 1UL, UK.

Show MeSH
Related in: MedlinePlus