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Determination of the ideal rat model for spinal cord injury by diffusion tensor imaging.

Wang F, Huang SL, He XJ, Li XH - Neuroreport (2014)

Bottom Line: We found that DTI tractography tracked nerve fibers and showed conspicuous changes in the injured spinal cord in all the model groups, which confirmed that our modeling was successful.A decrease in FA values and an increase in ADC were observed in all the model groups after surgery.Basso, Beattie, and Bresnahan scores further proved the results because of a significant, positive correlation of the scores with FA (R=0.899, P<0.01) and a significant, negative correlation of the scores with ADC (R=-0.829, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Departments of aOrthopaedics bRadiology, School of Medicine, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.

ABSTRACT
Four different spinal cord injury (SCI) models (hemisection, contusion, transection, and segment resection) were produced in male Sprague-Dawley rats to determine the most suitable animal model of SCI by analyzing the changes in diffusion tensor imaging (DTI) parameters both qualitatively and quantitatively in vivo. Radiological examinations were performed before surgery and weekly within 4 weeks after surgery to obtain DTI tractography, MRI routine images, and DTI data of fractional anisotropy (FA) and apparent diffusion coefficient (ADC). The Basso, Beattie, and Bresnahan scale was used to evaluate the locomotor outcomes. We found that DTI tractography tracked nerve fibers and showed conspicuous changes in the injured spinal cord in all the model groups, which confirmed that our modeling was successful. A decrease in FA values and an increase in ADC were observed in all the model groups after surgery. There were significant differences in FA and ADC between weeks 1 and 4 in both hemisection and contusion groups (P<0.05), whereas the differences in the transection and segment resection groups were not as remarkable (P>0.05). Basso, Beattie, and Bresnahan scores further proved the results because of a significant, positive correlation of the scores with FA (R=0.899, P<0.01) and a significant, negative correlation of the scores with ADC (R=-0.829, P<0.01). Therefore, the transection model, which is more quantified and stable within 4 weeks after injury according to the DTI and behavioral evaluation, should be used as the standard model for SCI animal testing.

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T1-weighted and T2-weighted images of the spinal cord of rats in different groups at week 1 postoperatively. (a, b) Control group; (c, d) hemisection group; (e, f) contusion group; (g, h) transection group; (i, j) segment resection group. The arrow indicates the injured site.
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Figure 1: T1-weighted and T2-weighted images of the spinal cord of rats in different groups at week 1 postoperatively. (a, b) Control group; (c, d) hemisection group; (e, f) contusion group; (g, h) transection group; (i, j) segment resection group. The arrow indicates the injured site.

Mentions: The conventional magnetic resonance T1-weighted and T2-weighted images of the control group presented an intact spinal cord and no obvious abnormalities were found in the signal intensity (Fig. 1a and b). For the four SCI model groups, the T1-weighted images vaguely showed the injured area whereas the T2-weighted images clearly visualized the cutting trace of the spinal cord and the presence of edema after contusion (Fig. 1c–j).


Determination of the ideal rat model for spinal cord injury by diffusion tensor imaging.

Wang F, Huang SL, He XJ, Li XH - Neuroreport (2014)

T1-weighted and T2-weighted images of the spinal cord of rats in different groups at week 1 postoperatively. (a, b) Control group; (c, d) hemisection group; (e, f) contusion group; (g, h) transection group; (i, j) segment resection group. The arrow indicates the injured site.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222712&req=5

Figure 1: T1-weighted and T2-weighted images of the spinal cord of rats in different groups at week 1 postoperatively. (a, b) Control group; (c, d) hemisection group; (e, f) contusion group; (g, h) transection group; (i, j) segment resection group. The arrow indicates the injured site.
Mentions: The conventional magnetic resonance T1-weighted and T2-weighted images of the control group presented an intact spinal cord and no obvious abnormalities were found in the signal intensity (Fig. 1a and b). For the four SCI model groups, the T1-weighted images vaguely showed the injured area whereas the T2-weighted images clearly visualized the cutting trace of the spinal cord and the presence of edema after contusion (Fig. 1c–j).

Bottom Line: We found that DTI tractography tracked nerve fibers and showed conspicuous changes in the injured spinal cord in all the model groups, which confirmed that our modeling was successful.A decrease in FA values and an increase in ADC were observed in all the model groups after surgery.Basso, Beattie, and Bresnahan scores further proved the results because of a significant, positive correlation of the scores with FA (R=0.899, P<0.01) and a significant, negative correlation of the scores with ADC (R=-0.829, P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Departments of aOrthopaedics bRadiology, School of Medicine, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.

ABSTRACT
Four different spinal cord injury (SCI) models (hemisection, contusion, transection, and segment resection) were produced in male Sprague-Dawley rats to determine the most suitable animal model of SCI by analyzing the changes in diffusion tensor imaging (DTI) parameters both qualitatively and quantitatively in vivo. Radiological examinations were performed before surgery and weekly within 4 weeks after surgery to obtain DTI tractography, MRI routine images, and DTI data of fractional anisotropy (FA) and apparent diffusion coefficient (ADC). The Basso, Beattie, and Bresnahan scale was used to evaluate the locomotor outcomes. We found that DTI tractography tracked nerve fibers and showed conspicuous changes in the injured spinal cord in all the model groups, which confirmed that our modeling was successful. A decrease in FA values and an increase in ADC were observed in all the model groups after surgery. There were significant differences in FA and ADC between weeks 1 and 4 in both hemisection and contusion groups (P<0.05), whereas the differences in the transection and segment resection groups were not as remarkable (P>0.05). Basso, Beattie, and Bresnahan scores further proved the results because of a significant, positive correlation of the scores with FA (R=0.899, P<0.01) and a significant, negative correlation of the scores with ADC (R=-0.829, P<0.01). Therefore, the transection model, which is more quantified and stable within 4 weeks after injury according to the DTI and behavioral evaluation, should be used as the standard model for SCI animal testing.

Show MeSH
Related in: MedlinePlus