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Characteristics of a rat model of an open craniocerebral injury at simulated high altitude.

Yu AY, Xu QH, Hu SL, Li F, Chen YJ, Yin Y, Zhu G, Lin JK, Feng H - Neuroreport (2014)

Bottom Line: In contrast, rCBF and PbtO2 had decreased markedly by 72 h (P<0.01); brainstem auditory-evoked potential values were significantly prolonged (P<0.05).Moreover, an inverse correlation between rCBF and BWC and a positive correlation between rCBF and PbtO2 were found.The rat model of an open craniocerebral injury at simulated high altitude can be established successfully using a nail gun shot and a hypobaric chamber.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing bThe 41 Hospital of PLA, Tibet, People's Republic of China.

ABSTRACT
To establish a rat model of an open craniocerebral injury at simulated high altitude and to examine the characteristics of this model. Rats were divided randomly into a normobaric group and a high-altitude group and their corresponding control groups. A rat model of an open craniocerebral injury was established with a nail gun shot. Simulated high-altitude conditions were established with a hypobaric chamber at 0.6 ATA to mimic pressure at an altitude of 4000 m. Mortality, brain water content (BWC), Evans blue content, pathology, regional cerebral blood flow (rCBF), partial pressure of brain tissue oxygen (PbtO2), and brainstem auditory-evoked potential were observed after injury. The mortality of the high-altitude group was significantly greater than that of the normobaric group within 72 h after injury (P<0.05). BWC and Evans blue content increased by 48 h after injury (P<0.05); pathological changes in damaged brains were more serious. In contrast, rCBF and PbtO2 had decreased markedly by 72 h (P<0.01); brainstem auditory-evoked potential values were significantly prolonged (P<0.05). Moreover, an inverse correlation between rCBF and BWC and a positive correlation between rCBF and PbtO2 were found. The rat model of an open craniocerebral injury at simulated high altitude can be established successfully using a nail gun shot and a hypobaric chamber. The injury characteristics at high altitude were more serious, rapid, and prolonged than those in the normobaric group.

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(a) Comparison of ipsilateral BAEP in the NI and HI groups. (b) Contralateral BAEP comparison in the NI and HI groups. *P<0.05 versus the NI group at the same time point. BAEP, brainstem auditory-evoked potential; HI, high-altitude injury group; NI, normobaric injury group.
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Figure 6: (a) Comparison of ipsilateral BAEP in the NI and HI groups. (b) Contralateral BAEP comparison in the NI and HI groups. *P<0.05 versus the NI group at the same time point. BAEP, brainstem auditory-evoked potential; HI, high-altitude injury group; NI, normobaric injury group.

Mentions: PL and IPL of each wave after injury in the HI and NI group were significantly prolonged, especially on the injured side in the HI group. PL of the I, III, and V waves and IPL extension of I–V wave were most obvious (P<0.05). PL of each wave on the contralateral side had a different extension. Although the changes in the HI group were consistent with the NI group, PL and IPL of each wave in the HI group were prolonged more significantly than those in the NI group (P<0.05; Fig. 6a and b).


Characteristics of a rat model of an open craniocerebral injury at simulated high altitude.

Yu AY, Xu QH, Hu SL, Li F, Chen YJ, Yin Y, Zhu G, Lin JK, Feng H - Neuroreport (2014)

(a) Comparison of ipsilateral BAEP in the NI and HI groups. (b) Contralateral BAEP comparison in the NI and HI groups. *P<0.05 versus the NI group at the same time point. BAEP, brainstem auditory-evoked potential; HI, high-altitude injury group; NI, normobaric injury group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222709&req=5

Figure 6: (a) Comparison of ipsilateral BAEP in the NI and HI groups. (b) Contralateral BAEP comparison in the NI and HI groups. *P<0.05 versus the NI group at the same time point. BAEP, brainstem auditory-evoked potential; HI, high-altitude injury group; NI, normobaric injury group.
Mentions: PL and IPL of each wave after injury in the HI and NI group were significantly prolonged, especially on the injured side in the HI group. PL of the I, III, and V waves and IPL extension of I–V wave were most obvious (P<0.05). PL of each wave on the contralateral side had a different extension. Although the changes in the HI group were consistent with the NI group, PL and IPL of each wave in the HI group were prolonged more significantly than those in the NI group (P<0.05; Fig. 6a and b).

Bottom Line: In contrast, rCBF and PbtO2 had decreased markedly by 72 h (P<0.01); brainstem auditory-evoked potential values were significantly prolonged (P<0.05).Moreover, an inverse correlation between rCBF and BWC and a positive correlation between rCBF and PbtO2 were found.The rat model of an open craniocerebral injury at simulated high altitude can be established successfully using a nail gun shot and a hypobaric chamber.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing bThe 41 Hospital of PLA, Tibet, People's Republic of China.

ABSTRACT
To establish a rat model of an open craniocerebral injury at simulated high altitude and to examine the characteristics of this model. Rats were divided randomly into a normobaric group and a high-altitude group and their corresponding control groups. A rat model of an open craniocerebral injury was established with a nail gun shot. Simulated high-altitude conditions were established with a hypobaric chamber at 0.6 ATA to mimic pressure at an altitude of 4000 m. Mortality, brain water content (BWC), Evans blue content, pathology, regional cerebral blood flow (rCBF), partial pressure of brain tissue oxygen (PbtO2), and brainstem auditory-evoked potential were observed after injury. The mortality of the high-altitude group was significantly greater than that of the normobaric group within 72 h after injury (P<0.05). BWC and Evans blue content increased by 48 h after injury (P<0.05); pathological changes in damaged brains were more serious. In contrast, rCBF and PbtO2 had decreased markedly by 72 h (P<0.01); brainstem auditory-evoked potential values were significantly prolonged (P<0.05). Moreover, an inverse correlation between rCBF and BWC and a positive correlation between rCBF and PbtO2 were found. The rat model of an open craniocerebral injury at simulated high altitude can be established successfully using a nail gun shot and a hypobaric chamber. The injury characteristics at high altitude were more serious, rapid, and prolonged than those in the normobaric group.

Show MeSH
Related in: MedlinePlus