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Use of whole genome expression analysis in the toxicity screening of nanoparticles.

Fröhlich E, Meindl C, Wagner K, Leitinger G, Roblegg E - Toxicol. Appl. Pharmacol. (2014)

Bottom Line: Application of lower particle doses and presence of protein decreased the total number of regulated genes but did not markedly influence the top regulated genes.It can be concluded that regulated functions correlated well with results in cell-based assays.Presence of protein mitigated cytotoxicity but did not cause a different pattern of regulated processes.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: eleonore.froehlich@medunigraz.at.

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Related in: MedlinePlus

List of the 20 top up-regulated genes (fold-change in red) and the 10 top down-regulated genes (fold-change in green). Out of all whole genome expression analyses datasets to study the role of size (a), cytotoxicity; particle concentration (b), and cytotoxicity/role of protein coating (c) are listed. Genes are categorized according to GeneCards® to their localization or biological function. Headings of the columns indicate exposure conditions: particle concentration and % of FBS in DMEM.
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f0020: List of the 20 top up-regulated genes (fold-change in red) and the 10 top down-regulated genes (fold-change in green). Out of all whole genome expression analyses datasets to study the role of size (a), cytotoxicity; particle concentration (b), and cytotoxicity/role of protein coating (c) are listed. Genes are categorized according to GeneCards® to their localization or biological function. Headings of the columns indicate exposure conditions: particle concentration and % of FBS in DMEM.

Mentions: Endothelial cell-specific genes (P- and E-selectin, VWF, VCAM-1, GAADD45B, MAPK, STAT, cyclins A and B), in general, were not regulated by the NPs used in this study. Listed among the top 20 up-regulated and the 10 top down-regulated genes, however, endothelin 1 (EDN1) was down-regulated after exposure to PPS20 (10% FBS), CPS20 (200 μg/ml) and SCNTc (50 μg/ml, Fig. 3a).


Use of whole genome expression analysis in the toxicity screening of nanoparticles.

Fröhlich E, Meindl C, Wagner K, Leitinger G, Roblegg E - Toxicol. Appl. Pharmacol. (2014)

List of the 20 top up-regulated genes (fold-change in red) and the 10 top down-regulated genes (fold-change in green). Out of all whole genome expression analyses datasets to study the role of size (a), cytotoxicity; particle concentration (b), and cytotoxicity/role of protein coating (c) are listed. Genes are categorized according to GeneCards® to their localization or biological function. Headings of the columns indicate exposure conditions: particle concentration and % of FBS in DMEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4222661&req=5

f0020: List of the 20 top up-regulated genes (fold-change in red) and the 10 top down-regulated genes (fold-change in green). Out of all whole genome expression analyses datasets to study the role of size (a), cytotoxicity; particle concentration (b), and cytotoxicity/role of protein coating (c) are listed. Genes are categorized according to GeneCards® to their localization or biological function. Headings of the columns indicate exposure conditions: particle concentration and % of FBS in DMEM.
Mentions: Endothelial cell-specific genes (P- and E-selectin, VWF, VCAM-1, GAADD45B, MAPK, STAT, cyclins A and B), in general, were not regulated by the NPs used in this study. Listed among the top 20 up-regulated and the 10 top down-regulated genes, however, endothelin 1 (EDN1) was down-regulated after exposure to PPS20 (10% FBS), CPS20 (200 μg/ml) and SCNTc (50 μg/ml, Fig. 3a).

Bottom Line: Application of lower particle doses and presence of protein decreased the total number of regulated genes but did not markedly influence the top regulated genes.It can be concluded that regulated functions correlated well with results in cell-based assays.Presence of protein mitigated cytotoxicity but did not cause a different pattern of regulated processes.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: eleonore.froehlich@medunigraz.at.

Show MeSH
Related in: MedlinePlus