Use of whole genome expression analysis in the toxicity screening of nanoparticles.
Bottom Line: Application of lower particle doses and presence of protein decreased the total number of regulated genes but did not markedly influence the top regulated genes.It can be concluded that regulated functions correlated well with results in cell-based assays.Presence of protein mitigated cytotoxicity but did not cause a different pattern of regulated processes.
Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: firstname.lastname@example.org.Show MeSH
Related in: MedlinePlus
Mentions: Endothelial cell-specific genes (P- and E-selectin, VWF, VCAM-1, GAADD45B, MAPK, STAT, cyclins A and B), in general, were not regulated by the NPs used in this study. Listed among the top 20 up-regulated and the 10 top down-regulated genes, however, endothelin 1 (EDN1) was down-regulated after exposure to PPS20 (10% FBS), CPS20 (200 μg/ml) and SCNTc (50 μg/ml, Fig. 3a).
Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: email@example.com.