Limits...
Use of whole genome expression analysis in the toxicity screening of nanoparticles.

Fröhlich E, Meindl C, Wagner K, Leitinger G, Roblegg E - Toxicol. Appl. Pharmacol. (2014)

Bottom Line: Application of lower particle doses and presence of protein decreased the total number of regulated genes but did not markedly influence the top regulated genes.It can be concluded that regulated functions correlated well with results in cell-based assays.Presence of protein mitigated cytotoxicity but did not cause a different pattern of regulated processes.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: eleonore.froehlich@medunigraz.at.

Show MeSH

Related in: MedlinePlus

Viability of EAhy926 cells assessed by formazan bioreduction (dehydrogenase activity) in the presence of NPs for 24 h. a: Small polystyrene particles (small dots) show stronger cytotoxicity than larger particles (larger dots). b: Thin CNTs (red and pink curves) show higher cytotoxicity than thick CNTs (green and blue curves). Viability is indicated related to the dehydrogenase activity of cells in the respective medium as 100%.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4222661&req=5

f0015: Viability of EAhy926 cells assessed by formazan bioreduction (dehydrogenase activity) in the presence of NPs for 24 h. a: Small polystyrene particles (small dots) show stronger cytotoxicity than larger particles (larger dots). b: Thin CNTs (red and pink curves) show higher cytotoxicity than thick CNTs (green and blue curves). Viability is indicated related to the dehydrogenase activity of cells in the respective medium as 100%.

Mentions: Exposure to 200 μg/ml PPS20 in DMEM + 10% FBS and to 200 μg/ml CPS20 particles in DMEM caused significant cytotoxicity after 24 h (Fig. 2a). After 4 h, 200 μg/ml PPS20 and 200 μg/ml CPS20 particles did not reduce viability significantly (data not shown). PPS20 particles suspended in DMEM without FBS reacted much more cytotoxic and 10 μg/ml PPS20 caused the same decrease in viability as 200 μg/ml PPS20 particles suspended in DMEM + 10% FBS. When CPS20 particles were assessed in DMEM + 1% FBS for 24 h, cytotoxicity was less pronounced than at the same concentration of CPS20 particles in DMEM without FBS; instead of 5 ± 0.5% viability at 200 μg/ml CPS20 particles after exposure to DMEM without FBS, viability was 68 ± 9% (data not shown). At 10% FBS in the medium no cytotoxicity was observed up to 1 mg/ml CPS20 particles (data not shown). 50 μg/ml SCNTc, being the most cytotoxic of the CNTs tested, caused no significant decrease in viability (Fig. 2b). To verify absence of cytotoxicity in the microarray experiments viability testing was performed in parallel (data not shown).


Use of whole genome expression analysis in the toxicity screening of nanoparticles.

Fröhlich E, Meindl C, Wagner K, Leitinger G, Roblegg E - Toxicol. Appl. Pharmacol. (2014)

Viability of EAhy926 cells assessed by formazan bioreduction (dehydrogenase activity) in the presence of NPs for 24 h. a: Small polystyrene particles (small dots) show stronger cytotoxicity than larger particles (larger dots). b: Thin CNTs (red and pink curves) show higher cytotoxicity than thick CNTs (green and blue curves). Viability is indicated related to the dehydrogenase activity of cells in the respective medium as 100%.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4222661&req=5

f0015: Viability of EAhy926 cells assessed by formazan bioreduction (dehydrogenase activity) in the presence of NPs for 24 h. a: Small polystyrene particles (small dots) show stronger cytotoxicity than larger particles (larger dots). b: Thin CNTs (red and pink curves) show higher cytotoxicity than thick CNTs (green and blue curves). Viability is indicated related to the dehydrogenase activity of cells in the respective medium as 100%.
Mentions: Exposure to 200 μg/ml PPS20 in DMEM + 10% FBS and to 200 μg/ml CPS20 particles in DMEM caused significant cytotoxicity after 24 h (Fig. 2a). After 4 h, 200 μg/ml PPS20 and 200 μg/ml CPS20 particles did not reduce viability significantly (data not shown). PPS20 particles suspended in DMEM without FBS reacted much more cytotoxic and 10 μg/ml PPS20 caused the same decrease in viability as 200 μg/ml PPS20 particles suspended in DMEM + 10% FBS. When CPS20 particles were assessed in DMEM + 1% FBS for 24 h, cytotoxicity was less pronounced than at the same concentration of CPS20 particles in DMEM without FBS; instead of 5 ± 0.5% viability at 200 μg/ml CPS20 particles after exposure to DMEM without FBS, viability was 68 ± 9% (data not shown). At 10% FBS in the medium no cytotoxicity was observed up to 1 mg/ml CPS20 particles (data not shown). 50 μg/ml SCNTc, being the most cytotoxic of the CNTs tested, caused no significant decrease in viability (Fig. 2b). To verify absence of cytotoxicity in the microarray experiments viability testing was performed in parallel (data not shown).

Bottom Line: Application of lower particle doses and presence of protein decreased the total number of regulated genes but did not markedly influence the top regulated genes.It can be concluded that regulated functions correlated well with results in cell-based assays.Presence of protein mitigated cytotoxicity but did not cause a different pattern of regulated processes.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, Stiftingtalstr. 24, 8010 Graz, Austria. Electronic address: eleonore.froehlich@medunigraz.at.

Show MeSH
Related in: MedlinePlus