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Association study of gene polymorphisms and depression with abnormal humor in traditional Uighur medicine.

Yusup A, Upur H, Abla A, Upur H - BMC Complement Altern Med (2013)

Bottom Line: In addition, the OR associated with the combination of Val66Met-Val/Val genotype plus the presence of -1019C allele was 8.393 for ABB compared with controls (OR 8.393; 95% CI 1.807 ~ 38.991; P = 0.003).Moreover, the OR associated with the presence of -Met plus -1019C alleles was 12.194 for ABB compared with controls (OR 12.194; 95% CI 1.433 ~ 103.776; P = 0.005).The OR associated with the presence of -1438C/C plus Val/Val genotypes was 7.738 for ABB compared with controls (OR 7.738; 95% CI 1.566 ~ 38.241; P = 0.005).

View Article: PubMed Central - HTML - PubMed

Affiliation: Collage of Traditional Uighur Medicine, Xinjiang Medical University, Xinyi Road 393, Urumqi, China. halmurat@263.net.

ABSTRACT

Background: According to the humor theory of Traditional Uighur Medicine (TUM), a same disease is classified into different abnormal humor types and corresponding methods are applied to treat the diseases according to the type of abnormal humor characteristics. To date the biological foundation of classification of diseases by humor theory has been little studied and the mechanism of action is still unclear. In the present study, we aimed to investigate the association between some related gene polymorphisms and depression with abnormal humor in TUM.

Methods: 201 cases of depression patients in a Uighur population were divided into two groups as: 107 cases of depression patients with abnormal black bile (ABB), 94 cases of depression patients with none abnormal black bile (nABB), and 50 healthy people were served as control group. Venous blood was used to isolate DNA samples, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used for genotyping of single nucleotide polymorphisms (SNPs). Polymorphisms in the serotonin 2A (5-HT2A) receptor gene, brain derived neurotrophic factor (BDNF), serotonin 1A (5-HT1A) receptor gene were investigated in each groups, respectively.

Results: The 5-HT2A A-1438G, 5-HT2A T102C, BDNF Val66Met, and 5-HT1A C-1019G gene polymorphisms showed significant association with ABB. However, no difference between nABB and controls was found for those genotype distribution and allele frequency. Moreover, the T102C and A1438G SNPs in the 5-HT2A receptor gene polymorphisms were in linkage disequilibrium. In addition, the OR associated with the combination of Val66Met-Val/Val genotype plus the presence of -1019C allele was 8.393 for ABB compared with controls (OR 8.393; 95% CI 1.807 ~ 38.991; P = 0.003). Moreover, the OR associated with the presence of -Met plus -1019C alleles was 12.194 for ABB compared with controls (OR 12.194; 95% CI 1.433 ~ 103.776; P = 0.005). The OR associated with the presence of -1438C/C plus Val/Val genotypes was 7.738 for ABB compared with controls (OR 7.738; 95% CI 1.566 ~ 38.241; P = 0.005).

Conclusion: It was concluded that there were significant relationship between the gene polymorphisms and classification of depression with abnormal humor in TUM. The 5-HT2A A-1438G, 5-HT2A T102C, BDNF Val66Met, and 5-HT1A C-1019G gene polymorphisms might predict the incidence of depression with ABB.

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Agarose gel eletrophoretogram of 5-HT2A T102C PCR product after digestion with MspI. The distribution of the 5-HT2A T102C polymorphism in nABB group and control group were in agreement with the Hardy-Weinberg equilibrium. There were no significant differences between groups (P > 0.05). Lane M1: 2000 bp DNA marker; Lane A, D, and G: TT; Lane B, E, and H: CC; Lane C, F, and I: TC; Lane M2: 50 bp DNA Ladder.
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Figure 2: Agarose gel eletrophoretogram of 5-HT2A T102C PCR product after digestion with MspI. The distribution of the 5-HT2A T102C polymorphism in nABB group and control group were in agreement with the Hardy-Weinberg equilibrium. There were no significant differences between groups (P > 0.05). Lane M1: 2000 bp DNA marker; Lane A, D, and G: TT; Lane B, E, and H: CC; Lane C, F, and I: TC; Lane M2: 50 bp DNA Ladder.

Mentions: Homozygous genotypes were identified by the presence of a single 342-bp band (TT), or bands of 217- and 125-bp (CC). The heterozygous genotype (TC) had all three bands (Figure 2).


Association study of gene polymorphisms and depression with abnormal humor in traditional Uighur medicine.

Yusup A, Upur H, Abla A, Upur H - BMC Complement Altern Med (2013)

Agarose gel eletrophoretogram of 5-HT2A T102C PCR product after digestion with MspI. The distribution of the 5-HT2A T102C polymorphism in nABB group and control group were in agreement with the Hardy-Weinberg equilibrium. There were no significant differences between groups (P > 0.05). Lane M1: 2000 bp DNA marker; Lane A, D, and G: TT; Lane B, E, and H: CC; Lane C, F, and I: TC; Lane M2: 50 bp DNA Ladder.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222500&req=5

Figure 2: Agarose gel eletrophoretogram of 5-HT2A T102C PCR product after digestion with MspI. The distribution of the 5-HT2A T102C polymorphism in nABB group and control group were in agreement with the Hardy-Weinberg equilibrium. There were no significant differences between groups (P > 0.05). Lane M1: 2000 bp DNA marker; Lane A, D, and G: TT; Lane B, E, and H: CC; Lane C, F, and I: TC; Lane M2: 50 bp DNA Ladder.
Mentions: Homozygous genotypes were identified by the presence of a single 342-bp band (TT), or bands of 217- and 125-bp (CC). The heterozygous genotype (TC) had all three bands (Figure 2).

Bottom Line: In addition, the OR associated with the combination of Val66Met-Val/Val genotype plus the presence of -1019C allele was 8.393 for ABB compared with controls (OR 8.393; 95% CI 1.807 ~ 38.991; P = 0.003).Moreover, the OR associated with the presence of -Met plus -1019C alleles was 12.194 for ABB compared with controls (OR 12.194; 95% CI 1.433 ~ 103.776; P = 0.005).The OR associated with the presence of -1438C/C plus Val/Val genotypes was 7.738 for ABB compared with controls (OR 7.738; 95% CI 1.566 ~ 38.241; P = 0.005).

View Article: PubMed Central - HTML - PubMed

Affiliation: Collage of Traditional Uighur Medicine, Xinjiang Medical University, Xinyi Road 393, Urumqi, China. halmurat@263.net.

ABSTRACT

Background: According to the humor theory of Traditional Uighur Medicine (TUM), a same disease is classified into different abnormal humor types and corresponding methods are applied to treat the diseases according to the type of abnormal humor characteristics. To date the biological foundation of classification of diseases by humor theory has been little studied and the mechanism of action is still unclear. In the present study, we aimed to investigate the association between some related gene polymorphisms and depression with abnormal humor in TUM.

Methods: 201 cases of depression patients in a Uighur population were divided into two groups as: 107 cases of depression patients with abnormal black bile (ABB), 94 cases of depression patients with none abnormal black bile (nABB), and 50 healthy people were served as control group. Venous blood was used to isolate DNA samples, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used for genotyping of single nucleotide polymorphisms (SNPs). Polymorphisms in the serotonin 2A (5-HT2A) receptor gene, brain derived neurotrophic factor (BDNF), serotonin 1A (5-HT1A) receptor gene were investigated in each groups, respectively.

Results: The 5-HT2A A-1438G, 5-HT2A T102C, BDNF Val66Met, and 5-HT1A C-1019G gene polymorphisms showed significant association with ABB. However, no difference between nABB and controls was found for those genotype distribution and allele frequency. Moreover, the T102C and A1438G SNPs in the 5-HT2A receptor gene polymorphisms were in linkage disequilibrium. In addition, the OR associated with the combination of Val66Met-Val/Val genotype plus the presence of -1019C allele was 8.393 for ABB compared with controls (OR 8.393; 95% CI 1.807 ~ 38.991; P = 0.003). Moreover, the OR associated with the presence of -Met plus -1019C alleles was 12.194 for ABB compared with controls (OR 12.194; 95% CI 1.433 ~ 103.776; P = 0.005). The OR associated with the presence of -1438C/C plus Val/Val genotypes was 7.738 for ABB compared with controls (OR 7.738; 95% CI 1.566 ~ 38.241; P = 0.005).

Conclusion: It was concluded that there were significant relationship between the gene polymorphisms and classification of depression with abnormal humor in TUM. The 5-HT2A A-1438G, 5-HT2A T102C, BDNF Val66Met, and 5-HT1A C-1019G gene polymorphisms might predict the incidence of depression with ABB.

Show MeSH
Related in: MedlinePlus