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Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease.

Wu M, Sugimura Y, Iwata K, Takaya N, Takamatsu D, Kobayashi M, Taylor D, Kimura K, Yoshiyama M - J. Insect Sci. (2014)

Bottom Line: Microbial flora from the gut of the Japanese honey bee, Apis cerana japonica F. (Hymenoptera: Apidae), were characterized and evaluated for their potential to inhibit the growth of Melissococcus plutonius corrig. (ex White) Bailey and Collins (Lactobacillales: Enterococcaceae), the causative agent of European foulbrood.Analysis of 16S rRNA gene sequences from 17 bacterial strains isolated by using a culture-dependent method revealed that most isolates belonged to Bacillus, Staphylococcus, and Pantoea.In addition, in vivo feeding assays revealed that isolate Acja3 decreased the mortality of honey bee larvae infected with M plutonius, suggesting that this bacterial strain could potentially be used as a probiotic agent against European foulbrood.

View Article: PubMed Central - PubMed

Affiliation: Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan tanghis.wu@gmail.com.

No MeSH data available.


Related in: MedlinePlus

Inhibitory activity of Acja3 isolate obtained from the gut of the Japanese honey bee, A. c. japonica, against the M. plutonius DAT561 strain. Diameters (mean ± SD) of inhibitory zones were calculated based on three independent experiments. BHI liquid medium and tetracycline (Tet) were used as negative and positive controls, respectively.
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f02_01: Inhibitory activity of Acja3 isolate obtained from the gut of the Japanese honey bee, A. c. japonica, against the M. plutonius DAT561 strain. Diameters (mean ± SD) of inhibitory zones were calculated based on three independent experiments. BHI liquid medium and tetracycline (Tet) were used as negative and positive controls, respectively.

Mentions: To investigate the growth inhibitory activity against the M. plutonius DAT561 strain, we subjected all of the gut bacterial isolates to an in vitro inhibitory zone assay. One of the isolates, Acja3, identified as a Bacillus strain, showed strong inhibitory activity against the DAT561 strain, and had an average zone diameter of 19.7 mm (Fig. 2). Such inhibitory activity was not observed in any of the other isolates. The positive control (tetracycline) had an average zone diameter of 37.3 mm.


Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease.

Wu M, Sugimura Y, Iwata K, Takaya N, Takamatsu D, Kobayashi M, Taylor D, Kimura K, Yoshiyama M - J. Insect Sci. (2014)

Inhibitory activity of Acja3 isolate obtained from the gut of the Japanese honey bee, A. c. japonica, against the M. plutonius DAT561 strain. Diameters (mean ± SD) of inhibitory zones were calculated based on three independent experiments. BHI liquid medium and tetracycline (Tet) were used as negative and positive controls, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222316&req=5

f02_01: Inhibitory activity of Acja3 isolate obtained from the gut of the Japanese honey bee, A. c. japonica, against the M. plutonius DAT561 strain. Diameters (mean ± SD) of inhibitory zones were calculated based on three independent experiments. BHI liquid medium and tetracycline (Tet) were used as negative and positive controls, respectively.
Mentions: To investigate the growth inhibitory activity against the M. plutonius DAT561 strain, we subjected all of the gut bacterial isolates to an in vitro inhibitory zone assay. One of the isolates, Acja3, identified as a Bacillus strain, showed strong inhibitory activity against the DAT561 strain, and had an average zone diameter of 19.7 mm (Fig. 2). Such inhibitory activity was not observed in any of the other isolates. The positive control (tetracycline) had an average zone diameter of 37.3 mm.

Bottom Line: Microbial flora from the gut of the Japanese honey bee, Apis cerana japonica F. (Hymenoptera: Apidae), were characterized and evaluated for their potential to inhibit the growth of Melissococcus plutonius corrig. (ex White) Bailey and Collins (Lactobacillales: Enterococcaceae), the causative agent of European foulbrood.Analysis of 16S rRNA gene sequences from 17 bacterial strains isolated by using a culture-dependent method revealed that most isolates belonged to Bacillus, Staphylococcus, and Pantoea.In addition, in vivo feeding assays revealed that isolate Acja3 decreased the mortality of honey bee larvae infected with M plutonius, suggesting that this bacterial strain could potentially be used as a probiotic agent against European foulbrood.

View Article: PubMed Central - PubMed

Affiliation: Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan tanghis.wu@gmail.com.

No MeSH data available.


Related in: MedlinePlus