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Norepinephrine and dopamine increase motility, biofilm formation, and virulence of Vibrio harveyi.

Yang Q, Anh ND, Bossier P, Defoirdt T - Front Microbiol (2014)

Bottom Line: Further, NE-induced effects could be neutralized by α-adrenergic antagonists or by the bacterial catecholamine receptor antagonist LED209, but not by β-adrenergic or dopaminergic antagonists.Dopa-induced effects could be neutralized by dopaminergic antagonists or LED209, but not by adrenergic antagonists.Together, our results indicate that catecholamine sensing increases the success of transmission of V. harveyi and that interfering with catecholamine sensing might be an interesting strategy to control vibriosis in aquaculture.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Aquaculture and Artemia Reference Center, Department of Animal Production, Ghent University Ghent, Belgium.

ABSTRACT
Vibrio harveyi is one of the major pathogens of aquatic organisms, affecting both vertebrates and invertebrates, and causes important losses in the aquaculture industry. In order to develop novel methods to control disease caused by this pathogen, we need to obtain a better understanding of pathogenicity mechanisms. Sensing of catecholamines increases both growth and production of virulence-related factors in pathogens of terrestrial animals and humans. However, at this moment, knowledge on the impact of catecholamines on the virulence of pathogens of aquatic organisms is lacking. In the present study, we report that in V. harveyi, norepinephrine (NE) and dopamine (Dopa) increased growth in serum-supplemented medium, siderophore production, swimming motility, and expression of genes involved in flagellar motility, biofilm formation, and exopolysaccharide production. Consistent with this, pretreatment of V. harveyi with catecholamines prior to inoculation into the rearing water resulted in significantly decreased survival of gnotobiotic brine shrimp larvae, when compared to larvae challenged with untreated V. harveyi. Further, NE-induced effects could be neutralized by α-adrenergic antagonists or by the bacterial catecholamine receptor antagonist LED209, but not by β-adrenergic or dopaminergic antagonists. Dopa-induced effects could be neutralized by dopaminergic antagonists or LED209, but not by adrenergic antagonists. Together, our results indicate that catecholamine sensing increases the success of transmission of V. harveyi and that interfering with catecholamine sensing might be an interesting strategy to control vibriosis in aquaculture. We hypothesize that upon tissue and/or hemocyte damage during infection, pathogens come into contact with elevated catecholamine levels, and that this stimulates the expression of virulence factors that are required to colonize a new host.

No MeSH data available.


Related in: MedlinePlus

Impact of catecholamines and catecholamine receptor antagonists on the growth of V. harveyi in LB35 broth containing 30% (v/v) serum. (A) NE and adrenergic antagonists. (B) Dopa and dopaminergic antagonists. The initial density of V. harveyi was 102 CFU/ml. Error bars represent the SD of three independent cultures.
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Figure 3: Impact of catecholamines and catecholamine receptor antagonists on the growth of V. harveyi in LB35 broth containing 30% (v/v) serum. (A) NE and adrenergic antagonists. (B) Dopa and dopaminergic antagonists. The initial density of V. harveyi was 102 CFU/ml. Error bars represent the SD of three independent cultures.

Mentions: The addition of NE or Dopa increased the growth of V. harveyi in Marine Broth containing 30% (v/v) serum (Figure 3) and resulted in a 2.4- and 2.1-fold increase in the growth rate of V. harveyi, respectively (0.59 ± 0.05 h-1 and 0.53 ± 0.04 h-1 in the presence of NE and Dopa, respectively, compared to 0.25 ± 0.03 h-1 for the untreated control). When compared to untreated cultures, the maximum turbidity was 1.6-fold higher for both NE and Dopa supplemented cultures. The catecholamines had no effect on growth of V. harveyi in medium without serum (data not shown).


Norepinephrine and dopamine increase motility, biofilm formation, and virulence of Vibrio harveyi.

Yang Q, Anh ND, Bossier P, Defoirdt T - Front Microbiol (2014)

Impact of catecholamines and catecholamine receptor antagonists on the growth of V. harveyi in LB35 broth containing 30% (v/v) serum. (A) NE and adrenergic antagonists. (B) Dopa and dopaminergic antagonists. The initial density of V. harveyi was 102 CFU/ml. Error bars represent the SD of three independent cultures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222227&req=5

Figure 3: Impact of catecholamines and catecholamine receptor antagonists on the growth of V. harveyi in LB35 broth containing 30% (v/v) serum. (A) NE and adrenergic antagonists. (B) Dopa and dopaminergic antagonists. The initial density of V. harveyi was 102 CFU/ml. Error bars represent the SD of three independent cultures.
Mentions: The addition of NE or Dopa increased the growth of V. harveyi in Marine Broth containing 30% (v/v) serum (Figure 3) and resulted in a 2.4- and 2.1-fold increase in the growth rate of V. harveyi, respectively (0.59 ± 0.05 h-1 and 0.53 ± 0.04 h-1 in the presence of NE and Dopa, respectively, compared to 0.25 ± 0.03 h-1 for the untreated control). When compared to untreated cultures, the maximum turbidity was 1.6-fold higher for both NE and Dopa supplemented cultures. The catecholamines had no effect on growth of V. harveyi in medium without serum (data not shown).

Bottom Line: Further, NE-induced effects could be neutralized by α-adrenergic antagonists or by the bacterial catecholamine receptor antagonist LED209, but not by β-adrenergic or dopaminergic antagonists.Dopa-induced effects could be neutralized by dopaminergic antagonists or LED209, but not by adrenergic antagonists.Together, our results indicate that catecholamine sensing increases the success of transmission of V. harveyi and that interfering with catecholamine sensing might be an interesting strategy to control vibriosis in aquaculture.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Aquaculture and Artemia Reference Center, Department of Animal Production, Ghent University Ghent, Belgium.

ABSTRACT
Vibrio harveyi is one of the major pathogens of aquatic organisms, affecting both vertebrates and invertebrates, and causes important losses in the aquaculture industry. In order to develop novel methods to control disease caused by this pathogen, we need to obtain a better understanding of pathogenicity mechanisms. Sensing of catecholamines increases both growth and production of virulence-related factors in pathogens of terrestrial animals and humans. However, at this moment, knowledge on the impact of catecholamines on the virulence of pathogens of aquatic organisms is lacking. In the present study, we report that in V. harveyi, norepinephrine (NE) and dopamine (Dopa) increased growth in serum-supplemented medium, siderophore production, swimming motility, and expression of genes involved in flagellar motility, biofilm formation, and exopolysaccharide production. Consistent with this, pretreatment of V. harveyi with catecholamines prior to inoculation into the rearing water resulted in significantly decreased survival of gnotobiotic brine shrimp larvae, when compared to larvae challenged with untreated V. harveyi. Further, NE-induced effects could be neutralized by α-adrenergic antagonists or by the bacterial catecholamine receptor antagonist LED209, but not by β-adrenergic or dopaminergic antagonists. Dopa-induced effects could be neutralized by dopaminergic antagonists or LED209, but not by adrenergic antagonists. Together, our results indicate that catecholamine sensing increases the success of transmission of V. harveyi and that interfering with catecholamine sensing might be an interesting strategy to control vibriosis in aquaculture. We hypothesize that upon tissue and/or hemocyte damage during infection, pathogens come into contact with elevated catecholamine levels, and that this stimulates the expression of virulence factors that are required to colonize a new host.

No MeSH data available.


Related in: MedlinePlus