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Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis.

Jeßberger N, Lu Y, Amon J, Titgemeyer F, Sonnewald S, Reid S, Burkovski A - BMC Res Notes (2013)

Bottom Line: This includes changes in the transcription of several hundred genes encoding e.g. transport proteins, proteins involved in nitrogen metabolism and regulation, energy generation and protein turnover.The specific nitrogen-related changes at the transcriptional level depend mainly on the presence of GlnR, while the AmtR protein controls only a small number of genes.M. smegmatis is able to metabolize a number of different nitrogen sources and nitrogen control in M. smegmatis is similar to control mechanisms characterized in streptomycetes, while the master regulator of nitrogen control in corynebacteria, AmtR, is plays a minor role in this regulatory network.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. aburkov@biologie.uni-erlangen.de.

ABSTRACT

Background: As other bacteria, Mycobacterium smegmatis needs adaption mechanisms to cope with changing nitrogen sources and to survive situations of nitrogen starvation. In the study presented here, transcriptome analyses were used to characterize the response of the bacterium to nitrogen starvation and to elucidate the role of specific transcriptional regulators.

Results: In response to nitrogen deprivation, a general starvation response is induced in M. smegmatis. This includes changes in the transcription of several hundred genes encoding e.g. transport proteins, proteins involved in nitrogen metabolism and regulation, energy generation and protein turnover. The specific nitrogen-related changes at the transcriptional level depend mainly on the presence of GlnR, while the AmtR protein controls only a small number of genes.

Conclusions: M. smegmatis is able to metabolize a number of different nitrogen sources and nitrogen control in M. smegmatis is similar to control mechanisms characterized in streptomycetes, while the master regulator of nitrogen control in corynebacteria, AmtR, is plays a minor role in this regulatory network.

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Functional catagories of the M. smegmatis nitrogen starvation stimulon. DNA microarray analyses were carried out with RNA isolated from nitrogen-supplied and nitrogen-starved wild-type cells. Genes with decreased (A) and increased (B) transcript levels in response to nitrogen starvation were classified according to functional categories [13].
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Figure 1: Functional catagories of the M. smegmatis nitrogen starvation stimulon. DNA microarray analyses were carried out with RNA isolated from nitrogen-supplied and nitrogen-starved wild-type cells. Genes with decreased (A) and increased (B) transcript levels in response to nitrogen starvation were classified according to functional categories [13].

Mentions: COG classification of genes with decreased mRNA level (Figure 1A) revealed that 50% of these were allocated to the major group metabolism, another 27% to the major category poorly characterized. 16% of the genes with decreased transcript amounts in the wild-type under nitrogen starvation belonged to the group information storage and processing and 7% correlated with the group cellular processes and signaling. Only 4% of these 231 genes are described as putatively involved in nitrogen metabolism.


Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis.

Jeßberger N, Lu Y, Amon J, Titgemeyer F, Sonnewald S, Reid S, Burkovski A - BMC Res Notes (2013)

Functional catagories of the M. smegmatis nitrogen starvation stimulon. DNA microarray analyses were carried out with RNA isolated from nitrogen-supplied and nitrogen-starved wild-type cells. Genes with decreased (A) and increased (B) transcript levels in response to nitrogen starvation were classified according to functional categories [13].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4222082&req=5

Figure 1: Functional catagories of the M. smegmatis nitrogen starvation stimulon. DNA microarray analyses were carried out with RNA isolated from nitrogen-supplied and nitrogen-starved wild-type cells. Genes with decreased (A) and increased (B) transcript levels in response to nitrogen starvation were classified according to functional categories [13].
Mentions: COG classification of genes with decreased mRNA level (Figure 1A) revealed that 50% of these were allocated to the major group metabolism, another 27% to the major category poorly characterized. 16% of the genes with decreased transcript amounts in the wild-type under nitrogen starvation belonged to the group information storage and processing and 7% correlated with the group cellular processes and signaling. Only 4% of these 231 genes are described as putatively involved in nitrogen metabolism.

Bottom Line: This includes changes in the transcription of several hundred genes encoding e.g. transport proteins, proteins involved in nitrogen metabolism and regulation, energy generation and protein turnover.The specific nitrogen-related changes at the transcriptional level depend mainly on the presence of GlnR, while the AmtR protein controls only a small number of genes.M. smegmatis is able to metabolize a number of different nitrogen sources and nitrogen control in M. smegmatis is similar to control mechanisms characterized in streptomycetes, while the master regulator of nitrogen control in corynebacteria, AmtR, is plays a minor role in this regulatory network.

View Article: PubMed Central - HTML - PubMed

Affiliation: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. aburkov@biologie.uni-erlangen.de.

ABSTRACT

Background: As other bacteria, Mycobacterium smegmatis needs adaption mechanisms to cope with changing nitrogen sources and to survive situations of nitrogen starvation. In the study presented here, transcriptome analyses were used to characterize the response of the bacterium to nitrogen starvation and to elucidate the role of specific transcriptional regulators.

Results: In response to nitrogen deprivation, a general starvation response is induced in M. smegmatis. This includes changes in the transcription of several hundred genes encoding e.g. transport proteins, proteins involved in nitrogen metabolism and regulation, energy generation and protein turnover. The specific nitrogen-related changes at the transcriptional level depend mainly on the presence of GlnR, while the AmtR protein controls only a small number of genes.

Conclusions: M. smegmatis is able to metabolize a number of different nitrogen sources and nitrogen control in M. smegmatis is similar to control mechanisms characterized in streptomycetes, while the master regulator of nitrogen control in corynebacteria, AmtR, is plays a minor role in this regulatory network.

Show MeSH
Related in: MedlinePlus