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MicroRNA-21 promotes cell growth and migration by targeting programmed cell death 4 gene in Kazakh's esophageal squamous cell carcinoma.

Liu T, Liu Q, Zheng S, Gao X, Lu M, Yang C, Dai F, Sheyhidin I, Lu X - Dis. Markers (2014)

Bottom Line: There are two main types of EC--squamous cell carcinoma (ESCC) and adenocarcinoma (EAC).Although some advances in the exploration of its possible etiological mechanism were made recently including behaviors and environmental risk factors as well as gene alterations, the molecular mechanism underlying ESCC carcinogenesis and progression remains poorly understood.It has been reported that miR-21 was upregulated in most malignant cancers, the proposed mechanism of which was through suppressing expression of programmed cell death 4 (PDCD4).

View Article: PubMed Central - PubMed

Affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China ; State Key Lab Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China.

ABSTRACT
Esophageal cancer (EC) is the eighth most common cancer worldwide and the sixth most common cause of cancer death. There are two main types of EC--squamous cell carcinoma (ESCC) and adenocarcinoma (EAC). Although some advances in the exploration of its possible etiological mechanism were made recently including behaviors and environmental risk factors as well as gene alterations, the molecular mechanism underlying ESCC carcinogenesis and progression remains poorly understood. It has been reported that miR-21 was upregulated in most malignant cancers, the proposed mechanism of which was through suppressing expression of programmed cell death 4 (PDCD4). In present study, it is firstly reported that miR-21 was upregulated in Kazakh's ESCC and that miR-21 played a negative role in regulating PDCD4 using in situ hybridization (ISH) and luciferase reporter approach. Morever, in model of ESCC xenografted nude mice, miR-21 maybe used as an effective target in the treatment. The present results demonstrated that miR-21 may be a potential therapeutic target in management of ESCC.

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Related in: MedlinePlus

miR-21 negatively correlated with PDCD4 expression in ESCC tissues. ((a), (b)) miR-21 was detected as a weak positive staining in normal epithelium, strongly positive staining of the tumor. ((c), (d)) PDCD4 was detected as a strongly positive staining in normal epithelium, weak positive staining of the tumor.
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fig3: miR-21 negatively correlated with PDCD4 expression in ESCC tissues. ((a), (b)) miR-21 was detected as a weak positive staining in normal epithelium, strongly positive staining of the tumor. ((c), (d)) PDCD4 was detected as a strongly positive staining in normal epithelium, weak positive staining of the tumor.

Mentions: To determine and visualize the expression status of miR-21 in Kazakh's ESCC and paired control, ISH was employed for its advantages in localization and qualitative expression in FFPE blocks in comparison with qRT-PCR techniques, which was adopted as classical approach in the detection of miRNAs. There were 50 paired Kazakh's ESCC tissues and matched adjacent normal controls were enrolled. MiR-21 was extremely significantly overexpressed in Kazakh's ESCC tissues in comparison with paired adjacent normal control (P < 0.001) (Table 1). In normal squamous epithelium, there was slightly positive staining for miR-21, whereas ESCC showed strongly positive staining (Figures 3(a) and 3(b)). The scramble sequence probe showed no significant staining in either ESCC or matched adjacent normal tissues.


MicroRNA-21 promotes cell growth and migration by targeting programmed cell death 4 gene in Kazakh's esophageal squamous cell carcinoma.

Liu T, Liu Q, Zheng S, Gao X, Lu M, Yang C, Dai F, Sheyhidin I, Lu X - Dis. Markers (2014)

miR-21 negatively correlated with PDCD4 expression in ESCC tissues. ((a), (b)) miR-21 was detected as a weak positive staining in normal epithelium, strongly positive staining of the tumor. ((c), (d)) PDCD4 was detected as a strongly positive staining in normal epithelium, weak positive staining of the tumor.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4221975&req=5

fig3: miR-21 negatively correlated with PDCD4 expression in ESCC tissues. ((a), (b)) miR-21 was detected as a weak positive staining in normal epithelium, strongly positive staining of the tumor. ((c), (d)) PDCD4 was detected as a strongly positive staining in normal epithelium, weak positive staining of the tumor.
Mentions: To determine and visualize the expression status of miR-21 in Kazakh's ESCC and paired control, ISH was employed for its advantages in localization and qualitative expression in FFPE blocks in comparison with qRT-PCR techniques, which was adopted as classical approach in the detection of miRNAs. There were 50 paired Kazakh's ESCC tissues and matched adjacent normal controls were enrolled. MiR-21 was extremely significantly overexpressed in Kazakh's ESCC tissues in comparison with paired adjacent normal control (P < 0.001) (Table 1). In normal squamous epithelium, there was slightly positive staining for miR-21, whereas ESCC showed strongly positive staining (Figures 3(a) and 3(b)). The scramble sequence probe showed no significant staining in either ESCC or matched adjacent normal tissues.

Bottom Line: There are two main types of EC--squamous cell carcinoma (ESCC) and adenocarcinoma (EAC).Although some advances in the exploration of its possible etiological mechanism were made recently including behaviors and environmental risk factors as well as gene alterations, the molecular mechanism underlying ESCC carcinogenesis and progression remains poorly understood.It has been reported that miR-21 was upregulated in most malignant cancers, the proposed mechanism of which was through suppressing expression of programmed cell death 4 (PDCD4).

View Article: PubMed Central - PubMed

Affiliation: Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China ; State Key Lab Incubation Base of Xinjiang Major Diseases Research, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China.

ABSTRACT
Esophageal cancer (EC) is the eighth most common cancer worldwide and the sixth most common cause of cancer death. There are two main types of EC--squamous cell carcinoma (ESCC) and adenocarcinoma (EAC). Although some advances in the exploration of its possible etiological mechanism were made recently including behaviors and environmental risk factors as well as gene alterations, the molecular mechanism underlying ESCC carcinogenesis and progression remains poorly understood. It has been reported that miR-21 was upregulated in most malignant cancers, the proposed mechanism of which was through suppressing expression of programmed cell death 4 (PDCD4). In present study, it is firstly reported that miR-21 was upregulated in Kazakh's ESCC and that miR-21 played a negative role in regulating PDCD4 using in situ hybridization (ISH) and luciferase reporter approach. Morever, in model of ESCC xenografted nude mice, miR-21 maybe used as an effective target in the treatment. The present results demonstrated that miR-21 may be a potential therapeutic target in management of ESCC.

Show MeSH
Related in: MedlinePlus