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A review of adipocyte lineage cells and dermal papilla cells in hair follicle regeneration.

Zhang P, Kling RE, Ravuri SK, Kokai LE, Rubin JP, Chai JK, Marra KG - J Tissue Eng (2014)

Bottom Line: Alopecia is an exceedingly prevalent problem effecting men and women of all ages.Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage.In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

ABSTRACT
Alopecia is an exceedingly prevalent problem effecting men and women of all ages. The standard of care for alopecia involves either transplanting existing hair follicles to bald areas or attempting to stimulate existing follicles with topical and/or oral medication. Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage. Innovative cell-based therapies have focused on the dermal papilla cell as a way to grow new hair in previously bald areas. However, despite this attention, many obstacles exist, including retention of dermal papilla inducing ability and maintenance of dermal papilla productivity after several passages of culture. The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability. In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

No MeSH data available.


Related in: MedlinePlus

The influence of adipose stem cell conditional medium (ASC-CM) on proliferation of cultured dermal papilla cells (DPCs). Proliferation of passage 6 DPCs cultured for 1–14 days in various ratio of ASC-CM (100%, 50%, or 0%/regular medium). Data are displayed in means ± s.d. of at least three (n = 9) independent experiments of each ratio or time point. One-way ANOVA, followed by Bonferroni’s multiple comparison test, compared with the DPC + regular medium group.ANOVA: analysis of variance.*p < 0.05; #p < 0.01. Bar, ISD.
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fig3-2041731414556850: The influence of adipose stem cell conditional medium (ASC-CM) on proliferation of cultured dermal papilla cells (DPCs). Proliferation of passage 6 DPCs cultured for 1–14 days in various ratio of ASC-CM (100%, 50%, or 0%/regular medium). Data are displayed in means ± s.d. of at least three (n = 9) independent experiments of each ratio or time point. One-way ANOVA, followed by Bonferroni’s multiple comparison test, compared with the DPC + regular medium group.ANOVA: analysis of variance.*p < 0.05; #p < 0.01. Bar, ISD.

Mentions: To study the promotion effect of ASCs on hair regeneration in vitro, two models are commonly used: one is directly contacting ASCs with hair cells,59,60 and the other is using the transwell technique80 or conditional medium from ASCs79,81 to simulate the situation of hair cells influenced by ASCs. Previous studies also suggested that the paracrine function of ASCs should be considered as an important mechanism to stimulate hair growth. The ASC-CM contains secretory factors derived from ASCs and might be a promising tool for hair growth promotion. Recently, we examined the influence of ASC-CM on the hair-inducing ability of rat DPCs. By culturing DPCs in ASC-CM, it was found that ASC-CM could significantly enhanced proliferation of DPCs in a concentration gradient manner (Figure 3). We also found that DPCs treated with 100% ASC-CM could increase the expression of ALP than those with 50% ASC-CM or regular DP medium. This may indicate ASC-CM could increase the hair-inducing ability of DPCs (Figure 4).


A review of adipocyte lineage cells and dermal papilla cells in hair follicle regeneration.

Zhang P, Kling RE, Ravuri SK, Kokai LE, Rubin JP, Chai JK, Marra KG - J Tissue Eng (2014)

The influence of adipose stem cell conditional medium (ASC-CM) on proliferation of cultured dermal papilla cells (DPCs). Proliferation of passage 6 DPCs cultured for 1–14 days in various ratio of ASC-CM (100%, 50%, or 0%/regular medium). Data are displayed in means ± s.d. of at least three (n = 9) independent experiments of each ratio or time point. One-way ANOVA, followed by Bonferroni’s multiple comparison test, compared with the DPC + regular medium group.ANOVA: analysis of variance.*p < 0.05; #p < 0.01. Bar, ISD.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4221925&req=5

fig3-2041731414556850: The influence of adipose stem cell conditional medium (ASC-CM) on proliferation of cultured dermal papilla cells (DPCs). Proliferation of passage 6 DPCs cultured for 1–14 days in various ratio of ASC-CM (100%, 50%, or 0%/regular medium). Data are displayed in means ± s.d. of at least three (n = 9) independent experiments of each ratio or time point. One-way ANOVA, followed by Bonferroni’s multiple comparison test, compared with the DPC + regular medium group.ANOVA: analysis of variance.*p < 0.05; #p < 0.01. Bar, ISD.
Mentions: To study the promotion effect of ASCs on hair regeneration in vitro, two models are commonly used: one is directly contacting ASCs with hair cells,59,60 and the other is using the transwell technique80 or conditional medium from ASCs79,81 to simulate the situation of hair cells influenced by ASCs. Previous studies also suggested that the paracrine function of ASCs should be considered as an important mechanism to stimulate hair growth. The ASC-CM contains secretory factors derived from ASCs and might be a promising tool for hair growth promotion. Recently, we examined the influence of ASC-CM on the hair-inducing ability of rat DPCs. By culturing DPCs in ASC-CM, it was found that ASC-CM could significantly enhanced proliferation of DPCs in a concentration gradient manner (Figure 3). We also found that DPCs treated with 100% ASC-CM could increase the expression of ALP than those with 50% ASC-CM or regular DP medium. This may indicate ASC-CM could increase the hair-inducing ability of DPCs (Figure 4).

Bottom Line: Alopecia is an exceedingly prevalent problem effecting men and women of all ages.Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage.In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

ABSTRACT
Alopecia is an exceedingly prevalent problem effecting men and women of all ages. The standard of care for alopecia involves either transplanting existing hair follicles to bald areas or attempting to stimulate existing follicles with topical and/or oral medication. Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage. Innovative cell-based therapies have focused on the dermal papilla cell as a way to grow new hair in previously bald areas. However, despite this attention, many obstacles exist, including retention of dermal papilla inducing ability and maintenance of dermal papilla productivity after several passages of culture. The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability. In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

No MeSH data available.


Related in: MedlinePlus