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A review of adipocyte lineage cells and dermal papilla cells in hair follicle regeneration.

Zhang P, Kling RE, Ravuri SK, Kokai LE, Rubin JP, Chai JK, Marra KG - J Tissue Eng (2014)

Bottom Line: Alopecia is an exceedingly prevalent problem effecting men and women of all ages.Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage.The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

ABSTRACT
Alopecia is an exceedingly prevalent problem effecting men and women of all ages. The standard of care for alopecia involves either transplanting existing hair follicles to bald areas or attempting to stimulate existing follicles with topical and/or oral medication. Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage. Innovative cell-based therapies have focused on the dermal papilla cell as a way to grow new hair in previously bald areas. However, despite this attention, many obstacles exist, including retention of dermal papilla inducing ability and maintenance of dermal papilla productivity after several passages of culture. The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability. In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

No MeSH data available.


Related in: MedlinePlus

Establishment of primary dermal papilla cells culture. (a) Hair follicles were identified in scalp skin and dissected free under a stereomicroscope. (b) After digestion in 0.25% collagenase at 37°C for 30 min, a whole hair follicle with full structure can be extracted with a forceps. Using the tip of a needle, pressure was applied to the base of the follicle dermal sheath which was cut open, and the dermal papilla was revealed. (c) The dermal papilla was peeled off with a small portion of dermal sheath tissue at its base and was transferred to a 35-mm dish and cultured undisturbed for 1 week. (d) After 7 days, early migration of dermal papilla cells from the explants was evident.
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fig2-2041731414556850: Establishment of primary dermal papilla cells culture. (a) Hair follicles were identified in scalp skin and dissected free under a stereomicroscope. (b) After digestion in 0.25% collagenase at 37°C for 30 min, a whole hair follicle with full structure can be extracted with a forceps. Using the tip of a needle, pressure was applied to the base of the follicle dermal sheath which was cut open, and the dermal papilla was revealed. (c) The dermal papilla was peeled off with a small portion of dermal sheath tissue at its base and was transferred to a 35-mm dish and cultured undisturbed for 1 week. (d) After 7 days, early migration of dermal papilla cells from the explants was evident.

Mentions: The most widely used method to isolate DP is surgical microdissection, which has been successfully established in rodent whisker follicles as well as in human hair follicles.32,33 For the purpose of hair follicle tissue engineering, an efficient method has been developed, which involves applying dispase and subsequent collagenase to the lower part of hair follicles.34 As contamination of DP cultures with other mesenchymal cells, epithelial cells, matrix, or adipose tissue is undesirable, the use of microdissection techniques over those of enzymatic digestion is preferred. The isolated DPs are seeded onto a culture flask and within 1 week, the outgrowth of DPCs can be observed with flattened and polygonal morphology (Figure 2).


A review of adipocyte lineage cells and dermal papilla cells in hair follicle regeneration.

Zhang P, Kling RE, Ravuri SK, Kokai LE, Rubin JP, Chai JK, Marra KG - J Tissue Eng (2014)

Establishment of primary dermal papilla cells culture. (a) Hair follicles were identified in scalp skin and dissected free under a stereomicroscope. (b) After digestion in 0.25% collagenase at 37°C for 30 min, a whole hair follicle with full structure can be extracted with a forceps. Using the tip of a needle, pressure was applied to the base of the follicle dermal sheath which was cut open, and the dermal papilla was revealed. (c) The dermal papilla was peeled off with a small portion of dermal sheath tissue at its base and was transferred to a 35-mm dish and cultured undisturbed for 1 week. (d) After 7 days, early migration of dermal papilla cells from the explants was evident.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4221925&req=5

fig2-2041731414556850: Establishment of primary dermal papilla cells culture. (a) Hair follicles were identified in scalp skin and dissected free under a stereomicroscope. (b) After digestion in 0.25% collagenase at 37°C for 30 min, a whole hair follicle with full structure can be extracted with a forceps. Using the tip of a needle, pressure was applied to the base of the follicle dermal sheath which was cut open, and the dermal papilla was revealed. (c) The dermal papilla was peeled off with a small portion of dermal sheath tissue at its base and was transferred to a 35-mm dish and cultured undisturbed for 1 week. (d) After 7 days, early migration of dermal papilla cells from the explants was evident.
Mentions: The most widely used method to isolate DP is surgical microdissection, which has been successfully established in rodent whisker follicles as well as in human hair follicles.32,33 For the purpose of hair follicle tissue engineering, an efficient method has been developed, which involves applying dispase and subsequent collagenase to the lower part of hair follicles.34 As contamination of DP cultures with other mesenchymal cells, epithelial cells, matrix, or adipose tissue is undesirable, the use of microdissection techniques over those of enzymatic digestion is preferred. The isolated DPs are seeded onto a culture flask and within 1 week, the outgrowth of DPCs can be observed with flattened and polygonal morphology (Figure 2).

Bottom Line: Alopecia is an exceedingly prevalent problem effecting men and women of all ages.Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage.The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

ABSTRACT
Alopecia is an exceedingly prevalent problem effecting men and women of all ages. The standard of care for alopecia involves either transplanting existing hair follicles to bald areas or attempting to stimulate existing follicles with topical and/or oral medication. Yet, these treatment options are fraught with problems of cost, side effects, and, most importantly, inadequate long-term hair coverage. Innovative cell-based therapies have focused on the dermal papilla cell as a way to grow new hair in previously bald areas. However, despite this attention, many obstacles exist, including retention of dermal papilla inducing ability and maintenance of dermal papilla productivity after several passages of culture. The use of adipocyte lineage cells, including adipose-derived stem cells, has shown promise as a cell-based solution to regulate hair regeneration and may help in maintaining or increasing dermal papilla cells inducing hair ability. In this review, we highlight recent advances in the understanding of the cellular contribution and regulation of dermal papilla cells and summarize adipocyte lineage cells in hair regeneration.

No MeSH data available.


Related in: MedlinePlus