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The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro.

Matoušková E, Mestak O - J Tissue Eng (2014)

Bottom Line: The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata significantly (p < 0.05) higher in comparison to all products studied, except Xenoderm.The results of this in vitro study show that the brands based on porcine dermal matrix possess the strongest effect on keratinocyte proliferation and stratification.Furthermore, the results indicate that in vitro evaluation of effects on epithelial growth may accelerate the development of new bio-engineering-based wound covers.

View Article: PubMed Central - PubMed

Affiliation: Prague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.

ABSTRACT
The purpose of this study was to compare, by means of in vitro cultivation technique, five marketed brands of wound covers used in the treatment of burns and other skin defects (Biobrane(®), Suprathel(®), Veloderm(®), Xe-Derma(®), and Xenoderm(®)) for their ability to stimulate the keratinocyte growth, stratification, and differentiation. In three independent experiments, human keratinocytes were grown on the tested covers in organotypic cultures by the 3T3 feeder layer technique. Vertical paraffin sections of the wound covers with keratinocytes were processed using hematoxylin-eosin staining and immunostaining for involucrin. Keratinocyte populations on the dressings were assessed for (1) number of keratinocyte strata (primary variable), (2) quantitative growth, (3) thickness of the keratinocyte layer, and (4) cell differentiation. The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata significantly (p < 0.05) higher in comparison to all products studied, except Xenoderm. However, in contrast to Xe-Derma, Xenoderm did not significantly differ from the other dressings. The results of this in vitro study show that the brands based on porcine dermal matrix possess the strongest effect on keratinocyte proliferation and stratification. The distinctive position of Xe-Derma may be related to its composition, where natural dermal fibers form a smooth surface, similar to the basement membrane. Furthermore, the results indicate that in vitro evaluation of effects on epithelial growth may accelerate the development of new bio-engineering-based wound covers.

No MeSH data available.


Related in: MedlinePlus

Results of the quantified variables, expressed as the percentage of difference between the value obtained for the respective covers and the mean value over all covers.
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fig3-2041731414554966: Results of the quantified variables, expressed as the percentage of difference between the value obtained for the respective covers and the mean value over all covers.

Mentions: As a convenient measure of each dressing’s relative position, the results of the respective dressings in terms of a percent difference from the average of all dressings in the experiment were calculated; the results are presented in Figure 3.


The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro.

Matoušková E, Mestak O - J Tissue Eng (2014)

Results of the quantified variables, expressed as the percentage of difference between the value obtained for the respective covers and the mean value over all covers.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4221924&req=5

fig3-2041731414554966: Results of the quantified variables, expressed as the percentage of difference between the value obtained for the respective covers and the mean value over all covers.
Mentions: As a convenient measure of each dressing’s relative position, the results of the respective dressings in terms of a percent difference from the average of all dressings in the experiment were calculated; the results are presented in Figure 3.

Bottom Line: The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata significantly (p < 0.05) higher in comparison to all products studied, except Xenoderm.The results of this in vitro study show that the brands based on porcine dermal matrix possess the strongest effect on keratinocyte proliferation and stratification.Furthermore, the results indicate that in vitro evaluation of effects on epithelial growth may accelerate the development of new bio-engineering-based wound covers.

View Article: PubMed Central - PubMed

Affiliation: Prague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.

ABSTRACT
The purpose of this study was to compare, by means of in vitro cultivation technique, five marketed brands of wound covers used in the treatment of burns and other skin defects (Biobrane(®), Suprathel(®), Veloderm(®), Xe-Derma(®), and Xenoderm(®)) for their ability to stimulate the keratinocyte growth, stratification, and differentiation. In three independent experiments, human keratinocytes were grown on the tested covers in organotypic cultures by the 3T3 feeder layer technique. Vertical paraffin sections of the wound covers with keratinocytes were processed using hematoxylin-eosin staining and immunostaining for involucrin. Keratinocyte populations on the dressings were assessed for (1) number of keratinocyte strata (primary variable), (2) quantitative growth, (3) thickness of the keratinocyte layer, and (4) cell differentiation. The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata significantly (p < 0.05) higher in comparison to all products studied, except Xenoderm. However, in contrast to Xe-Derma, Xenoderm did not significantly differ from the other dressings. The results of this in vitro study show that the brands based on porcine dermal matrix possess the strongest effect on keratinocyte proliferation and stratification. The distinctive position of Xe-Derma may be related to its composition, where natural dermal fibers form a smooth surface, similar to the basement membrane. Furthermore, the results indicate that in vitro evaluation of effects on epithelial growth may accelerate the development of new bio-engineering-based wound covers.

No MeSH data available.


Related in: MedlinePlus