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Phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger (Abelmoschus esculentus L.) in mice.

Doreddula SK, Bonam SR, Gaddam DP, Desu BS, Ramarao N, Pandy V - ScientificWorldJournal (2014)

Bottom Line: In addition, these extracts significantly reduced the blood glucose, corticosterone, cholesterol, and triglyceride levels elevated by acute restraint stress and also significantly increased the time spent in open arm in EPM and decreased the immobility time in FST.It has also been revealed that these extracts showed a significant antioxidant activity and no signs of toxicity or death up to a dose of 2000 mg/kg, p.o.These results suggest that the seed extracts of Abelmoschus esculentus L. possess antioxidant, antistress, and nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh 522 034, India.

ABSTRACT
Abelmoschus esculentus L. (ladies finger, okra) is a well-known tropical vegetable, widely planted from Africa to Asia and from South Europe to America. In the present study, we investigated the in vitro antioxidant capacity and in vivo protective effect of the aqueous and methanolic seed extracts of Abelmoschus esculentus against scopolamine-induced cognitive impairment using passive avoidance task and acute restraining stress-induced behavioural and biochemical changes using elevated plus maze (EPM) and forced swimming test (FST) in mice. Our results demonstrated that the pretreatment of mice with aqueous and methanolic seed extracts of Abelmoschus esculentus (200 mg/kg, p.o.) for seven days significantly (P < 0.01) attenuated scopolamine-induced cognitive impairment in the passive avoidance test. In addition, these extracts significantly reduced the blood glucose, corticosterone, cholesterol, and triglyceride levels elevated by acute restraint stress and also significantly increased the time spent in open arm in EPM and decreased the immobility time in FST. It has also been revealed that these extracts showed a significant antioxidant activity and no signs of toxicity or death up to a dose of 2000 mg/kg, p.o. These results suggest that the seed extracts of Abelmoschus esculentus L. possess antioxidant, antistress, and nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable.

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Related in: MedlinePlus

(a) Ferric reducing power, (b) DPPH radical scavenging activity of BHT, AE, and ME seed extracts of Abelmoschus esculentus. Data are representative of three independent experiments. Values are mean ± S.E.M. The significance of differences was analysed by two-way ANOVA followed by Bonferroni multiple comparisons test (AE versus ME, *P < 0.05; **P < 0.01; ME versus BHT, #P < 0.05; ###P < 0.001; AE versus BHT, $P < 0.05; $$P < 0.01; $$$P < 0.001). (a) Treatment: F (2, 30) = 20.62, P < 0.0001; concentration: F (4, 30) = 122.39, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 4.79, P < 0.001). (b). Treatment: F (2, 30) = 35.82, P < 0.0001; concentration: F (4, 30) = 158.48, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 1.12, n.s).
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fig3: (a) Ferric reducing power, (b) DPPH radical scavenging activity of BHT, AE, and ME seed extracts of Abelmoschus esculentus. Data are representative of three independent experiments. Values are mean ± S.E.M. The significance of differences was analysed by two-way ANOVA followed by Bonferroni multiple comparisons test (AE versus ME, *P < 0.05; **P < 0.01; ME versus BHT, #P < 0.05; ###P < 0.001; AE versus BHT, $P < 0.05; $$P < 0.01; $$$P < 0.001). (a) Treatment: F (2, 30) = 20.62, P < 0.0001; concentration: F (4, 30) = 122.39, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 4.79, P < 0.001). (b). Treatment: F (2, 30) = 35.82, P < 0.0001; concentration: F (4, 30) = 158.48, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 1.12, n.s).

Mentions: The reducing power of aqueous (AE) and methanolic (ME) seed extracts of Abelmoschus esculentus compared with reference BHT in FRAP assay has been shown in Figure 3(a). The reducing power of these extracts was observed in a dose-dependent manner. The maximum reducing power of these extracts was determined at 1 mg/mL.


Phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger (Abelmoschus esculentus L.) in mice.

Doreddula SK, Bonam SR, Gaddam DP, Desu BS, Ramarao N, Pandy V - ScientificWorldJournal (2014)

(a) Ferric reducing power, (b) DPPH radical scavenging activity of BHT, AE, and ME seed extracts of Abelmoschus esculentus. Data are representative of three independent experiments. Values are mean ± S.E.M. The significance of differences was analysed by two-way ANOVA followed by Bonferroni multiple comparisons test (AE versus ME, *P < 0.05; **P < 0.01; ME versus BHT, #P < 0.05; ###P < 0.001; AE versus BHT, $P < 0.05; $$P < 0.01; $$$P < 0.001). (a) Treatment: F (2, 30) = 20.62, P < 0.0001; concentration: F (4, 30) = 122.39, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 4.79, P < 0.001). (b). Treatment: F (2, 30) = 35.82, P < 0.0001; concentration: F (4, 30) = 158.48, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 1.12, n.s).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4221879&req=5

fig3: (a) Ferric reducing power, (b) DPPH radical scavenging activity of BHT, AE, and ME seed extracts of Abelmoschus esculentus. Data are representative of three independent experiments. Values are mean ± S.E.M. The significance of differences was analysed by two-way ANOVA followed by Bonferroni multiple comparisons test (AE versus ME, *P < 0.05; **P < 0.01; ME versus BHT, #P < 0.05; ###P < 0.001; AE versus BHT, $P < 0.05; $$P < 0.01; $$$P < 0.001). (a) Treatment: F (2, 30) = 20.62, P < 0.0001; concentration: F (4, 30) = 122.39, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 4.79, P < 0.001). (b). Treatment: F (2, 30) = 35.82, P < 0.0001; concentration: F (4, 30) = 158.48, P < 0.0001; interaction (treatment × concentration): F (8, 30 = 1.12, n.s).
Mentions: The reducing power of aqueous (AE) and methanolic (ME) seed extracts of Abelmoschus esculentus compared with reference BHT in FRAP assay has been shown in Figure 3(a). The reducing power of these extracts was observed in a dose-dependent manner. The maximum reducing power of these extracts was determined at 1 mg/mL.

Bottom Line: In addition, these extracts significantly reduced the blood glucose, corticosterone, cholesterol, and triglyceride levels elevated by acute restraint stress and also significantly increased the time spent in open arm in EPM and decreased the immobility time in FST.It has also been revealed that these extracts showed a significant antioxidant activity and no signs of toxicity or death up to a dose of 2000 mg/kg, p.o.These results suggest that the seed extracts of Abelmoschus esculentus L. possess antioxidant, antistress, and nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh 522 034, India.

ABSTRACT
Abelmoschus esculentus L. (ladies finger, okra) is a well-known tropical vegetable, widely planted from Africa to Asia and from South Europe to America. In the present study, we investigated the in vitro antioxidant capacity and in vivo protective effect of the aqueous and methanolic seed extracts of Abelmoschus esculentus against scopolamine-induced cognitive impairment using passive avoidance task and acute restraining stress-induced behavioural and biochemical changes using elevated plus maze (EPM) and forced swimming test (FST) in mice. Our results demonstrated that the pretreatment of mice with aqueous and methanolic seed extracts of Abelmoschus esculentus (200 mg/kg, p.o.) for seven days significantly (P < 0.01) attenuated scopolamine-induced cognitive impairment in the passive avoidance test. In addition, these extracts significantly reduced the blood glucose, corticosterone, cholesterol, and triglyceride levels elevated by acute restraint stress and also significantly increased the time spent in open arm in EPM and decreased the immobility time in FST. It has also been revealed that these extracts showed a significant antioxidant activity and no signs of toxicity or death up to a dose of 2000 mg/kg, p.o. These results suggest that the seed extracts of Abelmoschus esculentus L. possess antioxidant, antistress, and nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable.

Show MeSH
Related in: MedlinePlus