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Identification and evaluation of strain B37 of Bacillus subtilis antagonistic to sapstain fungi on poplar wood.

Zhang X, Zhao G, Li D, Li S, Hong Q - ScientificWorldJournal (2014)

Bottom Line: YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties.After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium.The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China ; Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.

ABSTRACT
Devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in China. We had isolated four poplar sapstain fungi, Ceratocystis adiposa Hz91, Lasiodiplodia theobromae YM0737, L. theobromae Fx46, and Fusarium sp. YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium. The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

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A neighbor-joining phylogenetic dendrogram based on 16S rRNA gene sequences showing the position of strain B37 among members of the genus Bacillus species. Numbers on branch nodes are percentage bootstrap values (100 resamplings). The “T” in the figure means “type strain.”
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fig4: A neighbor-joining phylogenetic dendrogram based on 16S rRNA gene sequences showing the position of strain B37 among members of the genus Bacillus species. Numbers on branch nodes are percentage bootstrap values (100 resamplings). The “T” in the figure means “type strain.”

Mentions: The results of the 16S rRNA of strain B37 sequencing and aligning with published sequences from the GenBank database demonstrated that strain B37 had a 99.3% similarity to Bacillus subtilis NCIB 3610T (GenBank accession number ABQL01000001) (CP000560) (Figure 4). According to morphology, physiological and biochemical characteristics, and 16S rRNA sequence, strain B37 was identified as Bacillus subtilis and 16S rRNA of strain B37 was deposited at GenBank under accession number JN656409. Strain B37 was storing at China General Microbiological Culture Collection Center (CGMCC number 4277).


Identification and evaluation of strain B37 of Bacillus subtilis antagonistic to sapstain fungi on poplar wood.

Zhang X, Zhao G, Li D, Li S, Hong Q - ScientificWorldJournal (2014)

A neighbor-joining phylogenetic dendrogram based on 16S rRNA gene sequences showing the position of strain B37 among members of the genus Bacillus species. Numbers on branch nodes are percentage bootstrap values (100 resamplings). The “T” in the figure means “type strain.”
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4221877&req=5

fig4: A neighbor-joining phylogenetic dendrogram based on 16S rRNA gene sequences showing the position of strain B37 among members of the genus Bacillus species. Numbers on branch nodes are percentage bootstrap values (100 resamplings). The “T” in the figure means “type strain.”
Mentions: The results of the 16S rRNA of strain B37 sequencing and aligning with published sequences from the GenBank database demonstrated that strain B37 had a 99.3% similarity to Bacillus subtilis NCIB 3610T (GenBank accession number ABQL01000001) (CP000560) (Figure 4). According to morphology, physiological and biochemical characteristics, and 16S rRNA sequence, strain B37 was identified as Bacillus subtilis and 16S rRNA of strain B37 was deposited at GenBank under accession number JN656409. Strain B37 was storing at China General Microbiological Culture Collection Center (CGMCC number 4277).

Bottom Line: YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties.After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium.The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China ; Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.

ABSTRACT
Devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in China. We had isolated four poplar sapstain fungi, Ceratocystis adiposa Hz91, Lasiodiplodia theobromae YM0737, L. theobromae Fx46, and Fusarium sp. YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium. The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

Show MeSH
Related in: MedlinePlus