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Mediators of mast cells in bullous pemphigoid and dermatitis herpetiformis.

Zebrowska A, Wagrowska-Danilewicz M, Danilewicz M, Stasikowska-Kanicka O, Kulczycka-Siennicka L, Wozniacka A, Waszczykowska E - Mediators Inflamm. (2014)

Bottom Line: Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals.Increased serum PAF levels were observed in BP patients.Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.

ABSTRACT
Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

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Related in: MedlinePlus

Immunoexpression of TNFα and MMP9 in epidermis and influx, 400x, immunohistochemistry. (a) Immunoexpression of TNFα in epidermis and influx, skin lesions (BP). (b) Immunoexpression of TNFα in epidermis, skin lesion (DH). (c) Immunoexpression of TNFα in epidermis, perilesional skin (BP). (d) Immunoexpression of TNFα in epidermis, perilesional skin (DH). (e) Negative immunoexpression of TNFα, normal skin. (f) Immunoexpression of MMP9 in epidermis and influx, skin lesions (BP). (g) Almost negative immunoexpression of MMP9, normal skin.
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fig2: Immunoexpression of TNFα and MMP9 in epidermis and influx, 400x, immunohistochemistry. (a) Immunoexpression of TNFα in epidermis and influx, skin lesions (BP). (b) Immunoexpression of TNFα in epidermis, skin lesion (DH). (c) Immunoexpression of TNFα in epidermis, perilesional skin (BP). (d) Immunoexpression of TNFα in epidermis, perilesional skin (DH). (e) Negative immunoexpression of TNFα, normal skin. (f) Immunoexpression of MMP9 in epidermis and influx, skin lesions (BP). (g) Almost negative immunoexpression of MMP9, normal skin.

Mentions: Moderate expression of TNFα was found in inflammation cells, the basal keratinocytes, and blister fluid (Figure 2). TNFα expression was also observed in BP (17/27) and DH (9/14) perilesional skin (Figure 2). Immunohistochemistry revealed TNFα expression to be absent in the skin biopsies from control patients (Figure 2).


Mediators of mast cells in bullous pemphigoid and dermatitis herpetiformis.

Zebrowska A, Wagrowska-Danilewicz M, Danilewicz M, Stasikowska-Kanicka O, Kulczycka-Siennicka L, Wozniacka A, Waszczykowska E - Mediators Inflamm. (2014)

Immunoexpression of TNFα and MMP9 in epidermis and influx, 400x, immunohistochemistry. (a) Immunoexpression of TNFα in epidermis and influx, skin lesions (BP). (b) Immunoexpression of TNFα in epidermis, skin lesion (DH). (c) Immunoexpression of TNFα in epidermis, perilesional skin (BP). (d) Immunoexpression of TNFα in epidermis, perilesional skin (DH). (e) Negative immunoexpression of TNFα, normal skin. (f) Immunoexpression of MMP9 in epidermis and influx, skin lesions (BP). (g) Almost negative immunoexpression of MMP9, normal skin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4221872&req=5

fig2: Immunoexpression of TNFα and MMP9 in epidermis and influx, 400x, immunohistochemistry. (a) Immunoexpression of TNFα in epidermis and influx, skin lesions (BP). (b) Immunoexpression of TNFα in epidermis, skin lesion (DH). (c) Immunoexpression of TNFα in epidermis, perilesional skin (BP). (d) Immunoexpression of TNFα in epidermis, perilesional skin (DH). (e) Negative immunoexpression of TNFα, normal skin. (f) Immunoexpression of MMP9 in epidermis and influx, skin lesions (BP). (g) Almost negative immunoexpression of MMP9, normal skin.
Mentions: Moderate expression of TNFα was found in inflammation cells, the basal keratinocytes, and blister fluid (Figure 2). TNFα expression was also observed in BP (17/27) and DH (9/14) perilesional skin (Figure 2). Immunohistochemistry revealed TNFα expression to be absent in the skin biopsies from control patients (Figure 2).

Bottom Line: Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals.Increased serum PAF levels were observed in BP patients.Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, Medical University of Lodz, Plac Hallera 1, 90-497 Lodz, Poland.

ABSTRACT
Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

Show MeSH
Related in: MedlinePlus