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Assessing the survival of exogenous plant microRNA in mice.

Liang G, Zhu Y, Sun B, Shao Y, Jing A, Wang J, Xiao Z - Food Sci Nutr (2014)

Bottom Line: The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate.Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally.The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing, 210096, China ; School of Medical Technology and Engineering, Henan University of Science and Technology Luo Yang 471003, Henan, China.

ABSTRACT
MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10-50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.

No MeSH data available.


Correlation between recovery of miR-172 and miR-824 in mice excrement.
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fig06: Correlation between recovery of miR-172 and miR-824 in mice excrement.

Mentions: In this context, many questions remain to be investigated, none of which will be technically easy to control. Our data are consistent with the notion that a steady flow of exogenous genetic material persists in the GI tract, and a very small percent can enter the circulation and distribute in different organs (Zhang et al. 2012). Understanding whether small RNA survival in mammals is specific to miR-172 or similar to other small RNA is of interest. While measuring miR-172, we also tested the presence of miR-824 after feeding 50 μg of RNA to mice, and the result suggests that there is a good correlation between miR-824 and miR-172 (Fig. 6). The present findings, however, must be reproduced with other types of small RNA.


Assessing the survival of exogenous plant microRNA in mice.

Liang G, Zhu Y, Sun B, Shao Y, Jing A, Wang J, Xiao Z - Food Sci Nutr (2014)

Correlation between recovery of miR-172 and miR-824 in mice excrement.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221836&req=5

fig06: Correlation between recovery of miR-172 and miR-824 in mice excrement.
Mentions: In this context, many questions remain to be investigated, none of which will be technically easy to control. Our data are consistent with the notion that a steady flow of exogenous genetic material persists in the GI tract, and a very small percent can enter the circulation and distribute in different organs (Zhang et al. 2012). Understanding whether small RNA survival in mammals is specific to miR-172 or similar to other small RNA is of interest. While measuring miR-172, we also tested the presence of miR-824 after feeding 50 μg of RNA to mice, and the result suggests that there is a good correlation between miR-824 and miR-172 (Fig. 6). The present findings, however, must be reproduced with other types of small RNA.

Bottom Line: The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate.Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally.The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing, 210096, China ; School of Medical Technology and Engineering, Henan University of Science and Technology Luo Yang 471003, Henan, China.

ABSTRACT
MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10-50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.

No MeSH data available.