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Assessing the survival of exogenous plant microRNA in mice.

Liang G, Zhu Y, Sun B, Shao Y, Jing A, Wang J, Xiao Z - Food Sci Nutr (2014)

Bottom Line: The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate.Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally.The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing, 210096, China ; School of Medical Technology and Engineering, Henan University of Science and Technology Luo Yang 471003, Henan, China.

ABSTRACT
MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10-50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.

No MeSH data available.


Plant RNA degradation in vitro at different time points. Equivalent amount (5 μg) of RNA was incubated with serum (1–5) or fecal suspension (6–9) at various times. 1, 2 h; 2, 4 h; 3, 10 h; 4, 24 h; 5, 36 h; 6, 2 h; 7, 4 h; 8, 10 h; 9,18 h. M, RNA marker RL1000; 10: synthesized 21nt RNA oligo.
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fig02: Plant RNA degradation in vitro at different time points. Equivalent amount (5 μg) of RNA was incubated with serum (1–5) or fecal suspension (6–9) at various times. 1, 2 h; 2, 4 h; 3, 10 h; 4, 24 h; 5, 36 h; 6, 2 h; 7, 4 h; 8, 10 h; 9,18 h. M, RNA marker RL1000; 10: synthesized 21nt RNA oligo.

Mentions: Despite the lack of a low-molecular weight RNA marker, we can infer that the bright bands on the agarose gel are small RNA fragments in the range 20∼100 bp that survived degradation after different times of incubation (Fig. 2). In serum, there were large amounts of RNAs that survived after 24 h of incubation. After 36 h incubation, however, only about 5% of the RNAs were detected. At later times (72 h), the existence of RNA could still be detected (data not shown). However, in fecal suspensions, all RNA molecules were undetectable after 18 h of incubation. These results suggested that small RNA in the serum is more resistant to degradation than in fecal suspensions.


Assessing the survival of exogenous plant microRNA in mice.

Liang G, Zhu Y, Sun B, Shao Y, Jing A, Wang J, Xiao Z - Food Sci Nutr (2014)

Plant RNA degradation in vitro at different time points. Equivalent amount (5 μg) of RNA was incubated with serum (1–5) or fecal suspension (6–9) at various times. 1, 2 h; 2, 4 h; 3, 10 h; 4, 24 h; 5, 36 h; 6, 2 h; 7, 4 h; 8, 10 h; 9,18 h. M, RNA marker RL1000; 10: synthesized 21nt RNA oligo.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221836&req=5

fig02: Plant RNA degradation in vitro at different time points. Equivalent amount (5 μg) of RNA was incubated with serum (1–5) or fecal suspension (6–9) at various times. 1, 2 h; 2, 4 h; 3, 10 h; 4, 24 h; 5, 36 h; 6, 2 h; 7, 4 h; 8, 10 h; 9,18 h. M, RNA marker RL1000; 10: synthesized 21nt RNA oligo.
Mentions: Despite the lack of a low-molecular weight RNA marker, we can infer that the bright bands on the agarose gel are small RNA fragments in the range 20∼100 bp that survived degradation after different times of incubation (Fig. 2). In serum, there were large amounts of RNAs that survived after 24 h of incubation. After 36 h incubation, however, only about 5% of the RNAs were detected. At later times (72 h), the existence of RNA could still be detected (data not shown). However, in fecal suspensions, all RNA molecules were undetectable after 18 h of incubation. These results suggested that small RNA in the serum is more resistant to degradation than in fecal suspensions.

Bottom Line: The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate.Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally.The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University Nanjing, 210096, China ; School of Medical Technology and Engineering, Henan University of Science and Technology Luo Yang 471003, Henan, China.

ABSTRACT
MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10-50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05-4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.

No MeSH data available.