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In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells.

Kang T, Guan R, Chen X, Song Y, Jiang H, Zhao J - Nanoscale Res Lett (2013)

Bottom Line: There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists.The IC50 value was found at a low concentration.The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, People's Republic of China. rongfaguan@163.com.

ABSTRACT
There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists. To assess size-dependent cytotoxicity on human cancer cells, we studied the cytotoxic effect of three kinds of zinc oxide nanoparticles (ZnO NPs) on human epithelial colorectal adenocarcinoma (Caco-2) cells. Nanoparticles were first characterized by size, distribution, and intensity. Multiple assays have been adopted to measure the cell activity and oxidative stress. The cytotoxicity of ZnO NPs was time dependent and dose dependent. The 24-h exposure was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system. The IC50 value was found at a low concentration. The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase. The toxicity resulted in a deletion of cells in the G1 phase and an accumulation of cells in the S and G2/M phases. One type of metallic oxide (ZnO) exerted different cytotoxic effects according to different particle sizes. Data from the previous experiments showed that 26-nm ZnO NPs appeared to have the highest toxicity to Caco-2 cells. The study demonstrated the toxicity of ZnO NPs to Caco-2 cells and the impact of particle size, which could be useful in the medical applications.

No MeSH data available.


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Percentage of Caco-2 cells evaluated by AO/EB. The data are presented as the mean of three independent experiments.
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Figure 4: Percentage of Caco-2 cells evaluated by AO/EB. The data are presented as the mean of three independent experiments.

Mentions: The acridine orange (AO)/ethidium bromide (EB) double staining principle combines the differential uptake of fluorescent DNA binding dyes acridine orange and ethidium bromide, and the morphological aspect of chromatin condensation in the stained nucleus [21]. The toxicity of ZnO NPs resulted in a dose-dependent decrease in the number of viable cells (VN) and a rise in early apoptotic cells (VA), late apoptotic cells (NVA), and necrotic cells (NVN) (Figure 4). The AO/EB assay is applicable for ZnO nanoparticles according to their cell membrane destabilization potential. Cultures exposed to 12.5 μg/ml ZnO NPs showed a decrease (70.5%, 84%, and 83% for 26-, 62-, and 90-nm ZnO NPs) in the number of viable cells when compared with the control (98.5%), with a concomitant increase in the number of early apoptotic cells (15%, 10%, and 10% for 26-, 62-, and 90-nm ZnO NPs). Cells exposed to a concentration of 50 μg/ml showed that late apoptotic cells and necrotic cells became the increasingly predominant cell type (Figure 5).


In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells.

Kang T, Guan R, Chen X, Song Y, Jiang H, Zhao J - Nanoscale Res Lett (2013)

Percentage of Caco-2 cells evaluated by AO/EB. The data are presented as the mean of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221644&req=5

Figure 4: Percentage of Caco-2 cells evaluated by AO/EB. The data are presented as the mean of three independent experiments.
Mentions: The acridine orange (AO)/ethidium bromide (EB) double staining principle combines the differential uptake of fluorescent DNA binding dyes acridine orange and ethidium bromide, and the morphological aspect of chromatin condensation in the stained nucleus [21]. The toxicity of ZnO NPs resulted in a dose-dependent decrease in the number of viable cells (VN) and a rise in early apoptotic cells (VA), late apoptotic cells (NVA), and necrotic cells (NVN) (Figure 4). The AO/EB assay is applicable for ZnO nanoparticles according to their cell membrane destabilization potential. Cultures exposed to 12.5 μg/ml ZnO NPs showed a decrease (70.5%, 84%, and 83% for 26-, 62-, and 90-nm ZnO NPs) in the number of viable cells when compared with the control (98.5%), with a concomitant increase in the number of early apoptotic cells (15%, 10%, and 10% for 26-, 62-, and 90-nm ZnO NPs). Cells exposed to a concentration of 50 μg/ml showed that late apoptotic cells and necrotic cells became the increasingly predominant cell type (Figure 5).

Bottom Line: There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists.The IC50 value was found at a low concentration.The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, People's Republic of China. rongfaguan@163.com.

ABSTRACT
There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists. To assess size-dependent cytotoxicity on human cancer cells, we studied the cytotoxic effect of three kinds of zinc oxide nanoparticles (ZnO NPs) on human epithelial colorectal adenocarcinoma (Caco-2) cells. Nanoparticles were first characterized by size, distribution, and intensity. Multiple assays have been adopted to measure the cell activity and oxidative stress. The cytotoxicity of ZnO NPs was time dependent and dose dependent. The 24-h exposure was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system. The IC50 value was found at a low concentration. The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase. The toxicity resulted in a deletion of cells in the G1 phase and an accumulation of cells in the S and G2/M phases. One type of metallic oxide (ZnO) exerted different cytotoxic effects according to different particle sizes. Data from the previous experiments showed that 26-nm ZnO NPs appeared to have the highest toxicity to Caco-2 cells. The study demonstrated the toxicity of ZnO NPs to Caco-2 cells and the impact of particle size, which could be useful in the medical applications.

No MeSH data available.


Related in: MedlinePlus