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In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells.

Kang T, Guan R, Chen X, Song Y, Jiang H, Zhao J - Nanoscale Res Lett (2013)

Bottom Line: There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists.The IC50 value was found at a low concentration.The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, People's Republic of China. rongfaguan@163.com.

ABSTRACT
There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists. To assess size-dependent cytotoxicity on human cancer cells, we studied the cytotoxic effect of three kinds of zinc oxide nanoparticles (ZnO NPs) on human epithelial colorectal adenocarcinoma (Caco-2) cells. Nanoparticles were first characterized by size, distribution, and intensity. Multiple assays have been adopted to measure the cell activity and oxidative stress. The cytotoxicity of ZnO NPs was time dependent and dose dependent. The 24-h exposure was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system. The IC50 value was found at a low concentration. The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase. The toxicity resulted in a deletion of cells in the G1 phase and an accumulation of cells in the S and G2/M phases. One type of metallic oxide (ZnO) exerted different cytotoxic effects according to different particle sizes. Data from the previous experiments showed that 26-nm ZnO NPs appeared to have the highest toxicity to Caco-2 cells. The study demonstrated the toxicity of ZnO NPs to Caco-2 cells and the impact of particle size, which could be useful in the medical applications.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity of ZnO NPs on Caco-2 cells. MTT assay. Cell viability of Caco-2 cells treated with different concentrations of different-sized ZnO NPs at different times. Exposure to ZnO NPs for (A) 12 h, (B) 24 h, and (C) 36 h. Results are expressed as the percentage of cell activity compared to the control. The data are presented as the mean ± SD of three independent experiments (n = 5).
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Figure 2: Cytotoxicity of ZnO NPs on Caco-2 cells. MTT assay. Cell viability of Caco-2 cells treated with different concentrations of different-sized ZnO NPs at different times. Exposure to ZnO NPs for (A) 12 h, (B) 24 h, and (C) 36 h. Results are expressed as the percentage of cell activity compared to the control. The data are presented as the mean ± SD of three independent experiments (n = 5).

Mentions: To assess the cell activity, the intracellular dose of formazan was quantified. Three different sizes of NPs were tested over a 12-, 24-, and 36-h exposure. As shown in Figure 2, the MTT results demonstrated that higher concentrations and longer incubation times generated more serious cytotoxicity. It was observed that the cell activity is statistically significantly different between the concentrations of 12.5 and 50 μg/ml for 24 h. For the data regarding the exposure to 26-nm ZnO NPs for 12 h, the percentage (%) MTT reduction (relative to control) of Caco-2 cells observed at concentrations of 25 and 50 μg/ml was 41.02% and 91.3%, respectively. The percentage of reduction was 25.3% and 58.1% after exposure to 62-nm ZnO NPs, and reduction was 42.11% and 90.7% after exposure to 90-nm ZnO NPs (Figure 2A). The 24-h value was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system [18-20]. The relevant IC50 values on Caco-2 cells were 15.55 ± 1.19 μg/ml, 22.84 ± 1.36 μg/ml, and 18.57 ± 1.27 μg/ml.


In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells.

Kang T, Guan R, Chen X, Song Y, Jiang H, Zhao J - Nanoscale Res Lett (2013)

Cytotoxicity of ZnO NPs on Caco-2 cells. MTT assay. Cell viability of Caco-2 cells treated with different concentrations of different-sized ZnO NPs at different times. Exposure to ZnO NPs for (A) 12 h, (B) 24 h, and (C) 36 h. Results are expressed as the percentage of cell activity compared to the control. The data are presented as the mean ± SD of three independent experiments (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221644&req=5

Figure 2: Cytotoxicity of ZnO NPs on Caco-2 cells. MTT assay. Cell viability of Caco-2 cells treated with different concentrations of different-sized ZnO NPs at different times. Exposure to ZnO NPs for (A) 12 h, (B) 24 h, and (C) 36 h. Results are expressed as the percentage of cell activity compared to the control. The data are presented as the mean ± SD of three independent experiments (n = 5).
Mentions: To assess the cell activity, the intracellular dose of formazan was quantified. Three different sizes of NPs were tested over a 12-, 24-, and 36-h exposure. As shown in Figure 2, the MTT results demonstrated that higher concentrations and longer incubation times generated more serious cytotoxicity. It was observed that the cell activity is statistically significantly different between the concentrations of 12.5 and 50 μg/ml for 24 h. For the data regarding the exposure to 26-nm ZnO NPs for 12 h, the percentage (%) MTT reduction (relative to control) of Caco-2 cells observed at concentrations of 25 and 50 μg/ml was 41.02% and 91.3%, respectively. The percentage of reduction was 25.3% and 58.1% after exposure to 62-nm ZnO NPs, and reduction was 42.11% and 90.7% after exposure to 90-nm ZnO NPs (Figure 2A). The 24-h value was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system [18-20]. The relevant IC50 values on Caco-2 cells were 15.55 ± 1.19 μg/ml, 22.84 ± 1.36 μg/ml, and 18.57 ± 1.27 μg/ml.

Bottom Line: There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists.The IC50 value was found at a low concentration.The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, People's Republic of China. rongfaguan@163.com.

ABSTRACT
There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists. To assess size-dependent cytotoxicity on human cancer cells, we studied the cytotoxic effect of three kinds of zinc oxide nanoparticles (ZnO NPs) on human epithelial colorectal adenocarcinoma (Caco-2) cells. Nanoparticles were first characterized by size, distribution, and intensity. Multiple assays have been adopted to measure the cell activity and oxidative stress. The cytotoxicity of ZnO NPs was time dependent and dose dependent. The 24-h exposure was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system. The IC50 value was found at a low concentration. The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase. The toxicity resulted in a deletion of cells in the G1 phase and an accumulation of cells in the S and G2/M phases. One type of metallic oxide (ZnO) exerted different cytotoxic effects according to different particle sizes. Data from the previous experiments showed that 26-nm ZnO NPs appeared to have the highest toxicity to Caco-2 cells. The study demonstrated the toxicity of ZnO NPs to Caco-2 cells and the impact of particle size, which could be useful in the medical applications.

No MeSH data available.


Related in: MedlinePlus