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Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase.

Pastorino L, Dellacasa E, Noor MR, Soulimane T, Bianchini P, D'Autilia F, Antipov A, Diaspro A, Tofail SA, Ruggiero C - PLoS ONE (2014)

Bottom Line: Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time.We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components.This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.

ABSTRACT
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

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Confocal images of NPCs in the presence of rhodamine.Forward (a, d), confocal (b, e) and merged (c, f) images of empty capsules (top) can be compared with the corresponding images of (PSS/PAH)4/CytcO NPCs (bottom). The scale bar is equivalent to 5 µm.
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pone-0112192-g004: Confocal images of NPCs in the presence of rhodamine.Forward (a, d), confocal (b, e) and merged (c, f) images of empty capsules (top) can be compared with the corresponding images of (PSS/PAH)4/CytcO NPCs (bottom). The scale bar is equivalent to 5 µm.

Mentions: Finally, the functionalized capsules were imaged by CLSM in order to characterize their permeability properties. Namely, the (PSS/PAH)4 and (PSS/PAH)4/CytcO NPCs were imaged in 20 mM Tris-HCl pH 8.5 in the presence of rhodamine. As expected, the (PSS/PAH)4 multilayered shell was found to be without defects and thus impermeable to the dye molecules, whereas the (PSS/PAH)4/CytcO multilayered shell was permeable (Figure 4).


Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase.

Pastorino L, Dellacasa E, Noor MR, Soulimane T, Bianchini P, D'Autilia F, Antipov A, Diaspro A, Tofail SA, Ruggiero C - PLoS ONE (2014)

Confocal images of NPCs in the presence of rhodamine.Forward (a, d), confocal (b, e) and merged (c, f) images of empty capsules (top) can be compared with the corresponding images of (PSS/PAH)4/CytcO NPCs (bottom). The scale bar is equivalent to 5 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221607&req=5

pone-0112192-g004: Confocal images of NPCs in the presence of rhodamine.Forward (a, d), confocal (b, e) and merged (c, f) images of empty capsules (top) can be compared with the corresponding images of (PSS/PAH)4/CytcO NPCs (bottom). The scale bar is equivalent to 5 µm.
Mentions: Finally, the functionalized capsules were imaged by CLSM in order to characterize their permeability properties. Namely, the (PSS/PAH)4 and (PSS/PAH)4/CytcO NPCs were imaged in 20 mM Tris-HCl pH 8.5 in the presence of rhodamine. As expected, the (PSS/PAH)4 multilayered shell was found to be without defects and thus impermeable to the dye molecules, whereas the (PSS/PAH)4/CytcO multilayered shell was permeable (Figure 4).

Bottom Line: Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time.We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components.This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.

ABSTRACT
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

Show MeSH