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Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase.

Pastorino L, Dellacasa E, Noor MR, Soulimane T, Bianchini P, D'Autilia F, Antipov A, Diaspro A, Tofail SA, Ruggiero C - PLoS ONE (2014)

Bottom Line: Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time.We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components.This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.

ABSTRACT
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

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Surface coverage grow-up of the multilayer (PAH/PSS)4/PAH/CytcO at pH 8.5 as a function of the number of layers, calculated with the Voigt model by QCM-D.Step number 11 correspond to surface coverage value of the multilayer after the addition of CytcO.
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pone-0112192-g002: Surface coverage grow-up of the multilayer (PAH/PSS)4/PAH/CytcO at pH 8.5 as a function of the number of layers, calculated with the Voigt model by QCM-D.Step number 11 correspond to surface coverage value of the multilayer after the addition of CytcO.

Mentions: For this reason, the data were analyzed using the Voigt-based model. In this model, the adsorbed film is represented by a single Voigt element consisting of a parallel combination of a spring and dashpot to represent the elastic (storage) and inelastic (damping) behavior of a material, respectively. The evolution of the surface coverage recorded during the deposition of the multilayer PAH-(PSS/PAH)4/CytcO is presented in Figure 2. The total thickness of the hydrated multilayer, constituting the NPC shell, was evaluated to be 45±3 nm, including the CytcO layer having a thickness of about 11 nm.


Multilayered polyelectrolyte microcapsules: interaction with the enzyme cytochrome C oxidase.

Pastorino L, Dellacasa E, Noor MR, Soulimane T, Bianchini P, D'Autilia F, Antipov A, Diaspro A, Tofail SA, Ruggiero C - PLoS ONE (2014)

Surface coverage grow-up of the multilayer (PAH/PSS)4/PAH/CytcO at pH 8.5 as a function of the number of layers, calculated with the Voigt model by QCM-D.Step number 11 correspond to surface coverage value of the multilayer after the addition of CytcO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221607&req=5

pone-0112192-g002: Surface coverage grow-up of the multilayer (PAH/PSS)4/PAH/CytcO at pH 8.5 as a function of the number of layers, calculated with the Voigt model by QCM-D.Step number 11 correspond to surface coverage value of the multilayer after the addition of CytcO.
Mentions: For this reason, the data were analyzed using the Voigt-based model. In this model, the adsorbed film is represented by a single Voigt element consisting of a parallel combination of a spring and dashpot to represent the elastic (storage) and inelastic (damping) behavior of a material, respectively. The evolution of the surface coverage recorded during the deposition of the multilayer PAH-(PSS/PAH)4/CytcO is presented in Figure 2. The total thickness of the hydrated multilayer, constituting the NPC shell, was evaluated to be 45±3 nm, including the CytcO layer having a thickness of about 11 nm.

Bottom Line: Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time.We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components.This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.

ABSTRACT
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties.

Show MeSH