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Evaluation of cotton leaf curl virus resistance in BC1, BC2, and BC3 progenies from an interspecific cross between Gossypium arboreum and Gossypium hirsutum.

Nazeer W, Tipu AL, Ahmad S, Mahmood K, Mahmood A, Zhou B - PLoS ONE (2014)

Bottom Line: The F1 progenies were completely resistant to this disease, while a decrease in resistance was observed in all backcross generations.Phenotypic resemblance of BC1 ∼BC3 progenies to G. arboreum confirmed the success of cross between the two species.Thus, this study successfully demonstrates the possibility of introgressing CLCuD resistance genes from G. arboreum to G. hirsutum.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germpalsm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu Province, China; Cotton Research Station, Multan, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan.

ABSTRACT
Cotton leaf curl virus disease (CLCuD) is an important constraint to cotton production. The resistance of G. arboreum to this devastating disease is well documented. In the present investigation, we explored the possibility of transferring genes for resistance to CLCuD from G. arboreum (2n = 26) cv 15-Mollisoni into G. hirsutum (2n = 52) cv CRSM-38 through conventional breeding. We investigated the cytology of the BC1 to BC3 progenies of direct and reciprocal crosses of G. arboreum and G. hirsutum and evaluated their resistance to CLCuD. The F1 progenies were completely resistant to this disease, while a decrease in resistance was observed in all backcross generations. As backcrossing progressed, the disease incidence increased in BC1 (1.7-2.0%), BC2 (1.8-4.0%), and BC3 (4.2-7.0%). However, the disease incidence was much lower than that of the check variety CIM-496, with a CLCuD incidence of 96%. Additionally, the disease incidence percentage was lower in the direct cross 2(G. arboreum)×G. hirsutum than in that of G. hirsutum×G. arboreum. Phenotypic resemblance of BC1 ∼BC3 progenies to G. arboreum confirmed the success of cross between the two species. Cytological studies of CLCuD-resistant plants revealed that the frequency of univalents and multivalents was high in BC1, with sterile or partially fertile plants, but low in BC2 (in both combinations), with shy bearing plants. In BC3, most of the plants exhibited normal bearing ability due to the high frequency of chromosome associations (bivalents). The assessment of CLCuD through grafting showed that the BC1 to BC3 progenies were highly resistant to this disease. Thus, this study successfully demonstrates the possibility of introgressing CLCuD resistance genes from G. arboreum to G. hirsutum.

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Advancement of boll setting and germination (%) across different generations (BC1 to BC3).D = Direct cross [2(G. arboreum)×G. hirsutum]; R = Reciprocal cross [G. hirsutum×G. arboreum].
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pone-0111861-g003: Advancement of boll setting and germination (%) across different generations (BC1 to BC3).D = Direct cross [2(G. arboreum)×G. hirsutum]; R = Reciprocal cross [G. hirsutum×G. arboreum].

Mentions: Examination of the cross ability of BC1 to BC3 of the combination 2(G. arboreum)×G. hirsutum and G. hirsutum×G. arboreum revealed that the maximum percentage of boll set (42.9%) and germination (67.0%) were observed in the BC3 (G. hirsutum×G. arboreum) progenies (Table 2). Minimum boll setting (1.3%) was recorded in the cross BC2 [2(G. arboreum)×G. hirsutum]. A minimum percentage of viable seeds (35.1%) were obtained in BC1 [2(G. arboreum)×G. hirsutum]. The boll setting and germination (%) gradually increased from BC1 to BC3 (Figure 3).


Evaluation of cotton leaf curl virus resistance in BC1, BC2, and BC3 progenies from an interspecific cross between Gossypium arboreum and Gossypium hirsutum.

Nazeer W, Tipu AL, Ahmad S, Mahmood K, Mahmood A, Zhou B - PLoS ONE (2014)

Advancement of boll setting and germination (%) across different generations (BC1 to BC3).D = Direct cross [2(G. arboreum)×G. hirsutum]; R = Reciprocal cross [G. hirsutum×G. arboreum].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4221200&req=5

pone-0111861-g003: Advancement of boll setting and germination (%) across different generations (BC1 to BC3).D = Direct cross [2(G. arboreum)×G. hirsutum]; R = Reciprocal cross [G. hirsutum×G. arboreum].
Mentions: Examination of the cross ability of BC1 to BC3 of the combination 2(G. arboreum)×G. hirsutum and G. hirsutum×G. arboreum revealed that the maximum percentage of boll set (42.9%) and germination (67.0%) were observed in the BC3 (G. hirsutum×G. arboreum) progenies (Table 2). Minimum boll setting (1.3%) was recorded in the cross BC2 [2(G. arboreum)×G. hirsutum]. A minimum percentage of viable seeds (35.1%) were obtained in BC1 [2(G. arboreum)×G. hirsutum]. The boll setting and germination (%) gradually increased from BC1 to BC3 (Figure 3).

Bottom Line: The F1 progenies were completely resistant to this disease, while a decrease in resistance was observed in all backcross generations.Phenotypic resemblance of BC1 ∼BC3 progenies to G. arboreum confirmed the success of cross between the two species.Thus, this study successfully demonstrates the possibility of introgressing CLCuD resistance genes from G. arboreum to G. hirsutum.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germpalsm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu Province, China; Cotton Research Station, Multan, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan.

ABSTRACT
Cotton leaf curl virus disease (CLCuD) is an important constraint to cotton production. The resistance of G. arboreum to this devastating disease is well documented. In the present investigation, we explored the possibility of transferring genes for resistance to CLCuD from G. arboreum (2n = 26) cv 15-Mollisoni into G. hirsutum (2n = 52) cv CRSM-38 through conventional breeding. We investigated the cytology of the BC1 to BC3 progenies of direct and reciprocal crosses of G. arboreum and G. hirsutum and evaluated their resistance to CLCuD. The F1 progenies were completely resistant to this disease, while a decrease in resistance was observed in all backcross generations. As backcrossing progressed, the disease incidence increased in BC1 (1.7-2.0%), BC2 (1.8-4.0%), and BC3 (4.2-7.0%). However, the disease incidence was much lower than that of the check variety CIM-496, with a CLCuD incidence of 96%. Additionally, the disease incidence percentage was lower in the direct cross 2(G. arboreum)×G. hirsutum than in that of G. hirsutum×G. arboreum. Phenotypic resemblance of BC1 ∼BC3 progenies to G. arboreum confirmed the success of cross between the two species. Cytological studies of CLCuD-resistant plants revealed that the frequency of univalents and multivalents was high in BC1, with sterile or partially fertile plants, but low in BC2 (in both combinations), with shy bearing plants. In BC3, most of the plants exhibited normal bearing ability due to the high frequency of chromosome associations (bivalents). The assessment of CLCuD through grafting showed that the BC1 to BC3 progenies were highly resistant to this disease. Thus, this study successfully demonstrates the possibility of introgressing CLCuD resistance genes from G. arboreum to G. hirsutum.

Show MeSH
Related in: MedlinePlus